Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | - |
Triticum aestivum |
General Stability | Organism |
---|---|
ethylene glycol stabilizes during purification, almost complete loss of activity without | Triticum aestivum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bicarbonate | - |
Triticum aestivum | |
Cl- | - |
Triticum aestivum | |
additional information | no inhibition by acetate, carbonate | Triticum aestivum | |
NO3- | no inhibition | Triticum aestivum | |
phosphate | in the forward reaction only in presence of fructose 2,6-bisphosphate | Triticum aestivum | |
SO42- | no inhibition | Triticum aestivum | |
sulfite | - |
Triticum aestivum | |
thiosulfate | - |
Triticum aestivum | |
tungstate | - |
Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | 0.033 | diphosphate | - |
Triticum aestivum | |
0.129 | - |
phosphate | - |
Triticum aestivum | |
0.139 | 0.21 | D-fructose 1,6-bisphosphate | - |
Triticum aestivum | |
0.3 | 0.38 | D-fructose 6-phosphate | - |
Triticum aestivum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | no activation of forward reaction | Triticum aestivum | |
Co2+ | no activitaion | Triticum aestivum | |
Mg2+ | requirement | Triticum aestivum | |
additional information | no activation by Cu2+ | Triticum aestivum | |
additional information | no activation by Ba2+, Sn2+, Sr2+ | Triticum aestivum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
1 * 80000 + 1 * 90000, SDS-PAGE | Triticum aestivum |
90000 | - |
1 * 80000 + 1 * 90000, SDS-PAGE | Triticum aestivum |
170000 | - |
gel filtration | Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | Triticum aestivum | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | - |
cv. WH-157 | - |
Purification (Comment) | Organism |
---|---|
partial, 127fold | Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Triticum aestivum | - |
seed | germinating | Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.5 | - |
partially purified enzyme | Triticum aestivum |
Storage Stability | Organism |
---|---|
-20°C, in 50 mM HEPES buffer, pH 7.5, 10 mM 2-mercaptoethanol, 0.5 mM EDTA, 1 mM MgCl2, 5 mM KCl, 30% ethylene glycol, more than 1 month | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | - |
Triticum aestivum | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Triticum aestivum | phosphate + D-fructose 1,6-bisphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 80000 + 1 * 90000, SDS-PAGE | Triticum aestivum |
Synonyms | Comment | Organism |
---|---|---|
PFP | - |
Triticum aestivum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
15 min stable | Triticum aestivum |
50 | - |
t1/2: 15 min | Triticum aestivum |
60 | - |
above, complete inactivation | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
forward reaction, D-fructose 2,6-bisphosphate has no effect on pH-optimum | Triticum aestivum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.78 | - |
phosphate | versus fructose 6-phosphate, pH 7.5, 25°C, in presence of fructose 2,6-bisphosphate at saturating concentration | Triticum aestivum | |
1.2 | - |
phosphate | versus diphosphate, pH 7.5, 25°C, in presence of fructose 2,6-bisphosphate at saturating concentration | Triticum aestivum |