Information on EC 2.7.1.90 - diphosphate-fructose-6-phosphate 1-phosphotransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.7.1.90
-
RECOMMENDED NAME
GeneOntology No.
diphosphate-fructose-6-phosphate 1-phosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycolysis IV (plant cytosol)
-
-
glycolysis
-
-
Glycolysis / Gluconeogenesis
-
-
Pentose phosphate pathway
-
-
Fructose and mannose metabolism
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
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Microbial metabolism in diverse environments
-
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Biosynthesis of antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
diphosphate:D-fructose-6-phosphate 1-phosphotransferase
The enzyme catalyses a similar reaction to EC 2.7.1.11, 6-phosphofructokinase, but utilizes diphosphate instead of ATP as the the phosphate donor. It has been described in higher plants, primitive eukaryotes, bacteria, and archaea.
CAS REGISTRY NUMBER
COMMENTARY hide
55326-40-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain B-PG9
-
-
Manually annotated by BRENDA team
strain B-PG9
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
enzyme is completely replaced by an ATP-dependent phosphofructokinase, when cells grow on only one C1-carbon source, e.g. methanol
Uniprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
var. botrytis
-
-
Manually annotated by BRENDA team
not induced by 0.1 M NaCl
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain Rt46 B.1
-
-
Manually annotated by BRENDA team
strain Rt46 B.1
-
-
Manually annotated by BRENDA team
DKB-strain
-
-
Manually annotated by BRENDA team
strain H200
-
-
Manually annotated by BRENDA team
strain Portland-1, ATCC 30888
-
-
Manually annotated by BRENDA team
cv. Mandarin
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain L1
-
-
Manually annotated by BRENDA team
strain L1
-
-
Manually annotated by BRENDA team
Musa cavendishii
-
-
-
Manually annotated by BRENDA team
KVL-strain
-
-
Manually annotated by BRENDA team
KVL-strain
-
-
Manually annotated by BRENDA team
B G-6-strain
-
-
Manually annotated by BRENDA team
B G-6-strain
-
-
Manually annotated by BRENDA team
no activity in Acanthamoeba castellani
-
-
-
Manually annotated by BRENDA team
no activity in Acanthamoeba castellani NEFF
-
-
-
Manually annotated by BRENDA team
no activity in Mycoplasma gallisepticum S6
-
-
-
Manually annotated by BRENDA team
no activity in Rhodopseudomonas capsulata
-
-
-
Manually annotated by BRENDA team
no activity in Rhodopseudomonas capsulata Kb1
-
-
-
Manually annotated by BRENDA team
no activity in Rhodopseudomonas palustris
-
-
-
Manually annotated by BRENDA team
no activity in Rhodopseudomonas palustris strain 1a1
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-
-
Manually annotated by BRENDA team
no activity in Rhodopseudomonas sphaeroides
-
-
-
Manually annotated by BRENDA team
no activity in Rhodopseudomonas viridis
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-
-
Manually annotated by BRENDA team
no activity in Spiroplasma floricola
-
-
-
Manually annotated by BRENDA team
no activity in Spiroplasma floricola 23-6
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
2 enzyme forms
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-
Manually annotated by BRENDA team
cv. Top Crop
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
strain S1
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-
Manually annotated by BRENDA team
strain S1
-
-
Manually annotated by BRENDA team
var. Hale
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Saccharum spp.
hybrid variety NCo310
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-
Manually annotated by BRENDA team
cv. Super First
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
PFP is important in carbohydrate metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(polyphosphate)n + D-fructose 6-phosphate
(polyphosphate)n-1 + D-fructose 1,6-bisphosphate
show the reaction diagram
arsenate + D-fructose 1,6-bisphosphate
?
show the reaction diagram
diphosphate + 2,5-anhydro-D-mannitol 6-phosphate
phosphate + 2,5-anhydro-D-mannitol 1,6-bisphosphate
show the reaction diagram
diphosphate + D-fructose 6-phosphate
phosphate + D-fructose 1,6-bisphosphate
show the reaction diagram
diphosphate + D-fructose 6-phosphate
phosphate + D-fructose 1,6-diphosphate
show the reaction diagram
diphosphate + D-ribulose 5-phosphate
?
show the reaction diagram
-
1.4% activity compared to D-fructose 6-phosphate
-
-
?
diphosphate + D-ribulose 5-phosphate
phosphate + ?
show the reaction diagram
-
-
-
-
?
diphosphate + D-sedoheptulose 6-phosphate
phosphate + D-sedoheptulose 1,6-bisposphate
show the reaction diagram
diphosphate + D-sedoheptulose 7-phosphate
phosphate + D-sedoheptulose 1,7-bisphosphate
show the reaction diagram
diphosphate + sedoheptulose 7-phosphate
phosphate + ?
show the reaction diagram
-
-
-
-
?
phosphate + D-fructose 1,6-bisphosphate
diphosphate + D-fructose 6-phosphate
show the reaction diagram
phosphate + D-fructose 1,6-diphosphate
diphosphate + D-fructose 6-phosphate
show the reaction diagram
phosphate + D-glucose 1,6-bisphosphate
?
show the reaction diagram
triphosphate + D-fructose 6-phosphate
diphosphate + D-fructose 1,6-bisphosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
diphosphate + D-fructose 6-phosphate
phosphate + D-fructose 1,6-bisphosphate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
the enzyme acts ATP-independent
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activates 120% at 0.1 mM
Divalent cations
Fe2+
-
activates 120% at 0.1 mM
Na+
-
activates 135% at 10 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,n-Alkanediol biphosphates
-
2,3-O-isopropylidene-alpha-L-sorbofuranose-1-phosphate
2,5-anhydro-D-glucitol-6-phosphate
2,5-Anhydro-D-mannitol 1,6-bisphosphate
-
D-fructose 1,6-bisphosphate-analog, kinetics
3-phospho-D-glycerate
3-phosphoglycerate
-
-
3-[N-(2-phenylthioethyl)-N-methylamino]-1-hydroxypropylidene-1,1-bisphosphonate
i.e. CGP 48048, bisphosphonate, synthetic diphosphate analogue; inhibition of growth
alendronate
bisphosphonate, synthetic diphosphate analogue; inhibition of growth
bicarbonate
-
-
citrate
clodronate
bisphosphonate, synthetic diphosphate analogue; inhibition of initial growth after inoculation
D-arabinose-5-phosphate
D-fructose 1,6-bisphosphate
D-fructose 2,6-bisphosphate
D-fructose 6-phosphate
D-glucitol-6-phosphate
D-glucose 6-phosphate
D-tagatose 6-phosphate
-
kinetics, dead-end substrate
D-tagatose-6-phosphate
diethylene glycol-monophosphate
diphosphate
etidronate
bisphosphonate, synthetic diphosphate analogue; inhibition of growth
Fe3+
-
84% inhibition at 0.1 mM
Imidodiphosphate
Increased ionic strength
-
e.g. 30 mM KCl, NH4Cl, 10 mM Na2SO4, (NH4)2SO4 or 5 mM sodium phosphate
-
KCl
-
complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction
L-sorbose-6-phosphate
magnesium diphosphate
-
reverse reaction, product inhibition, kinetics
malate
Methylenediphosphonate
nucleotides
-
-
-
pamidronate
bisphosphonate, synthetic diphosphate analogue; inhibition of growth
phosphate
phosphoenolpyruvate
Phosphoglycolate
-
-
risedronate
bisphosphonate, synthetic diphosphate analogue; inhibition of growth
Se4+
-
75% inhibition at 0.1 mM
SO42-
sulfate
-
weak
Tetrapolyphosphate
-
-
thiosulfate
-
-
tungstate
-
-
zoledronate
bisphosphonate, synthetic diphosphate analogue; inhibition of initial growth after inoculation
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine
-
slight activation
Aminoimidazolecarboxamide riboside
-
about 25% as efficient as AMP
citrate
CMP
-
slight activation
D-fructose 1,6-diphosphate
Musa cavendishii
-
up to 24fold enhancement of glycolytic enzyme activity
D-fructose 2,6-bisphosphate
D-glucose
D-Glucose 1,6-bisphosphate
-
-
D-glucose 1-phosphate
dAMP
-
activation, about 25% as efficient as AMP
GMP
-
activation, about 35% as efficient as AMP
IMP
-
activation, about 35% as efficient as AMP
MgADP-
-
activation, about 15% as efficient as AMP
MgATP2-
-
activation, about 15% as efficient as AMP
NaCl
-
100 mM, increase in activity to 150% of control, inhibitory above 150 mM
NAD+
-
slight activation
phosphate
-
slight activation
Polyethylene glycol
-
stimulation, synergistic with AMP
UMP
-
slight activation
XMP
-
slight activation
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.92 - 0.97
2,5-anhydro-D-mannitol-6-phosphate
3.6
arsenate
0.009 - 2.9
D-fructose 1,6-bisphosphate
0.025 - 0.082
D-fructose 1,6-diphosphate
0.01 - 4600
D-fructose 6-phosphate
0.27
D-fructose1,6-bisphosphate
-
pH 8.0, 37C
1.01
D-sedoheptulose 6-phosphate
-
in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30C
0.21 - 0.51
D-sedoheptulose 7-phosphate
0.005 - 0.58
diphosphate
0.023
fructose 1,6-bisphosphate
-
reverse reaction, pH 7.8, 25C
0.008 - 1.9
Mg2+
0.129 - 8.69
phosphate
0.027 - 0.49
sedoheptulose 7-phosphate
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06 - 330
D-fructose 6-phosphate
83 - 330
diphosphate
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.22
1,6-hexanediol-monophosphate
-
forward reaction, pH 8.0, 25C
2.37 - 6.7
2,3-O-isopropylidene-alpha-L-sorbofuranose-1-phosphate
0.32 - 1.52
2,5-anhydro-D-glucitol-6-phosphate
0.25
3-phosphoglycerate
-
pH 7.5
0.05
3-[N-(2-phenylthioethyl)-N-methylamino]-1-hydroxypropylidene-1,1-bisphosphonate
pH 7.0, 25C
0.4
alendronate
pH 7.0, 25C
20.2 - 56.9
Cl-
85
clodronate
pH 7.0, 25C
3.62
D-arabinose-5-phosphate
-
forward reaction, pH 8.0, 25C
0.013 - 0.086
D-fructose 1,6-bisphosphate
3.24 - 7.36
D-fructose 2,6-bisphosphate
0.113 - 0.62
D-fructose 6-phosphate
2.16 - 5.07
D-glucitol-6-phosphate
2.41 - 2.56
D-tagatose-6-phosphate
1.49 - 10.87
diethylene glycol-monophosphate
0.0025 - 1.3
diphosphate
0.56
etidronate
pH 7.0, 25C
2.05 - 2.39
L-sorbose-6-phosphate
0.39 - 10.3
phosphate
0.3
risedronate
pH 7.0, 25C
0.05
zoledronate
pH 7.0, 25C
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00016
-
crude enzyme extract, phosphor donor in the forward reaction is diphosphate
0.00021
-
crude enzyme extract, phosphor donor in the forward reaction is ATP
0.095
-
freshly sliced tissue samples, i.e. wounded, in presence of fructose 2,6-bisphosphate
0.101
-
sliced tissue samples, i.e. wounded, after 24 h, in presence of fructose 2,6-bisphosphate
0.136
purified recombinant His-tagged enzyme
0.2 - 0.6
-
partially purified enzyme
0.24
partially purified native enzyme
1.5
-
partially purified enzyme
1.72
-
partially purified enzyme
2
-
purified enzyme
3.5
-
purified enzyme, assay temperature 50C is not the temperature optimum
5.2
-
purified enzyme, in presence of D-fructose 2,6-bisphosphate at 0.001 mM
6.5
-
partially purified enzyme
8.25
-
purified enzyme
8.4
-
partially purified enzyme
8.47
-
partially purified enzyme
8.9
-
reverse reaction, purified enzyme, pH 7.7, in presence of fructose 2,6-bisphosphate
10
-
purified enzyme
13.6
-
partially purified enzyme
13.9
Musa cavendishii
-
pH 7.0, 25C
24.2
-
partially purified enzyme
24.4
-
purified enzyme
26
-
purified enzyme
30.2
-
purified enzyme
36.3
-
purified enzyme
40
-
partially purified enzyme
43.8
-
forward reaction, purified enzyme, pH 7.7, in presence of fructose 2,6-bisphosphate
45.7
-
purified enzyme
48.6
-
purified enzyme
65
-
purified enzyme
74 - 82
-
purified enzyme
112
-
37C, pH 6.0
146
-
purified enzyme
278
purified recombinant enzyme
298
-
37C, pH 7.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7 - 6.3
-
50C, forward reaction
6
-
forward reaction
6.7 - 8.7
-
reverse reaction, pH-independent, broad optimum
6.7 - 8
-
glycolytic and gluconeogenic reactions. D-fructose 2,6-bisphosphate shifts the pH optimum for the forward reaction to a slightly more basic pH
6.8
-
forward reaction in presence of fructose 2,6-bisphosphate
7 - 7.5
-
50C, reverse reaction
7.1
Musa cavendishii
-
braod
7.3 - 7.7
-
forward reaction
7.5
-
assay at; reverse reaction
7.5 - 7.8
-
forward and reverse reaction
7.5 - 8.5
-
forward reaction
7.5
-
forward reaction, D-fructose 2,6-bisphosphate has no effect on pH-optimum
7.7 - 7.9
-
forward reaction, strongly pH-dependent
7.7
-
both reaction directions
7.8 - 8.3
-
reverse reaction
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