Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | 50% activation of wild-type at 0.04 mM, of mutation P52L, large subunit, at 0.27 mM, of mutation P52L, large subunit, plus mutation L46F, small subunit at 0.1 mM, of mutation P52L, large subunit, plus mutation P112L, small subunit at 0.14 mM, of mutation P52L, large subunit, plus mutation P308L, small subunit at 0.006 mM, of mutation P52L, large subunit, plus mutation R350K, small subunit at 0.01 mM | Solanum tuberosum |
Protein Variants | Comment | Organism |
---|---|---|
L46F | mutation of small subunit, coexpression with mutation P52L of large subunit, partly restores sensitivity to 3-phosphoglycerate | Solanum tuberosum |
P112L | mutation of small subunit, coexpression with mutation P52L of large subunit, partly restores sensitivity to 3-phosphoglycerate | Solanum tuberosum |
P308L | mutation of small subunit, coexpression with mutation P52L of large subunit, partly restores sensitivity to 3-phosphoglycerate | Solanum tuberosum |
P52L | mutation in large subunit, mutant is 5fold less sensitive to activation by 3-phosphoglycerate | Solanum tuberosum |
R350K | mutation of small subunit, coexpression with mutation P52L of large subunit, partly restores sensitivity to 3-phosphoglycerate | Solanum tuberosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | at 0.5 mM 3-phosphoglycerate, 50% inhibition of wild-type at 0.54 mM, of mutation P52L, large subunit, at 0.52 mM, of mutation P52L, large subunit, plus mutation L46F, small subunit at 0.61 mM, of mutation P52L, large subunit, plus mutation P112L, small subunit at 0.43 mM, of mutation P52L, large subunit, plus mutation P308L, small subunit at 2.5 mM, of mutation P52L, large subunit, plus mutation R350K, small subunit at 1.9 mM | Solanum tuberosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
alpha-D-glucose 1-phosphate | wild-type, 37°C, pH 7.0 | Solanum tuberosum | |
0.17 | - |
ATP | wild-type, 37°C, pH 7.0 | Solanum tuberosum | |
0.21 | - |
alpha-D-glucose 1-phosphate | mutation P52L, large subunit, plus mutation P112L, small subunit, 37°C, pH 7.0 | Solanum tuberosum | |
0.22 | - |
ATP | mutation P52L, large subunit, plus mutation P112L, small subunit, 37°C, pH 7.0 | Solanum tuberosum | |
0.24 | - |
ATP | mutation P52L, large subunit, plus mutation L46F, small subunit, 37°C, pH 7.0 | Solanum tuberosum | |
0.24 | - |
ATP | mutation P52L, large subunit, plus mutation P308L, small subunit, 37°C, pH 7.0 | Solanum tuberosum | |
0.25 | - |
ATP | mutation P52L, large subunit, 37°C, pH 7.0 | Solanum tuberosum | |
0.29 | - |
ATP | mutation P52L, large subunit, plus mutation R350K, small subunit, 37°C, pH 7.0 | Solanum tuberosum | |
0.36 | - |
alpha-D-glucose 1-phosphate | mutation P52L, large subunit, plus mutation L46F, small subunit, 37°C, pH 7.0 | Solanum tuberosum | |
0.39 | - |
alpha-D-glucose 1-phosphate | mutation P52L, large subunit, plus mutation P308L, small subunit, 37°C, pH 7.0 | Solanum tuberosum | |
0.41 | - |
alpha-D-glucose 1-phosphate | mutation P52L, large subunit, plus mutation R350K, small subunit, 37°C, pH 7.0 | Solanum tuberosum | |
0.42 | - |
alpha-D-glucose 1-phosphate | mutation P52L, large subunit, 37°C, pH 7.0 | Solanum tuberosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tuber | - |
Solanum tuberosum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | ADP-D-glucose + diphosphate | - |
r | |
diphosphate + ADP | - |
Solanum tuberosum | ATP + alpha-D-glucose | - |
r |