EC Number |
Protein Variants |
Reference |
---|
2.7.6.5 | C633A |
20fold decrease in activity |
661218 |
2.7.6.5 | D264G |
eliminates detectable synthetase activity without appreciably altering the hydrolase activity |
661439 |
2.7.6.5 | D264G |
site-directed mutagenesis, the mutation specifically abolishes (p)ppGpp synthetase activity without affecting other potential functions of the protein. Loss of the (p)ppGpp synthetase activity results in failure to grow on minimal medium and requirement for valine, leucine, isoleucine, threonine, and methionine, and a weaker requirement for arginine, histidine, and tryptophan addition |
-, 738529 |
2.7.6.5 | D632A |
3.5fold decrease in activity |
661218 |
2.7.6.5 | D72G |
site-directed mutagenesis, the mutation specifically abolishes (p)ppGpp synthetase activity without affecting other potential functions of the protein. Loss of the (p)ppGpp synthetase activity results in failure to grow on minimal medium and requirement for valine, leucine, isoleucine, threonine, and methionine, and a weaker requirement for arginine, histidine, and tryptophan addition |
-, 738529 |
2.7.6.5 | D81A |
loss of hydrolytic activity with retention of synthesis |
661218 |
2.7.6.5 | D87G |
site-directed mutagenesis, the mutation specifically abolishes (p)ppGpp synthetase activity without affecting other potential functions of the protein. Loss of the (p)ppGpp synthetase activity results in failure to grow on minimal medium and requirement for valine, leucine, isoleucine, threonine, and methionine, and a weaker requirement for arginine, histidine, and tryptophan addition |
-, 738529 |
2.7.6.5 | E319Q |
mutant lacks (p)ppGpp synthase activity but retains hydrolase activity |
750643 |
2.7.6.5 | E323Q |
eliminates detectable synthetase activity without appreciably altering the hydrolase activity |
661439 |
2.7.6.5 | E324V |
inactive in (p)ppGpp synthesis |
-, 760841 |