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0.017 - 0.042
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
0.069
(3alpha,5beta,7beta)-3,7-dihydroxycholan-24-oic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.0027
(5beta,20R)-20-hydroxypregnan-3-one
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.022 - 0.14
1-Acenaphthenol
0.002 - 0.0024
17beta-hydroxy-5alpha-androstan-3-one
0.0007 - 0.0017
17beta-hydroxy-5beta-androstan-3-one
0.0007 - 0.00075
3-oxo-5beta-cholan-24-oic acid
-
-
0.0031
3alpha-androstanediol
-
pH 7.0, 25°C, recombinant enzyme
0.0014
3alpha-hydroxy-5alpha-pregnan-20-one
-
oxidation
0.005
3alpha-tetrahydroprogesterone
-
-
0.2 - 0.8
4-Nitroacetophenone
-
also in this range: 2,3-hexanedione, 1-phenyl-1,2-propanedione, 2,3-pentanedione, pyridine-4-aldehyde, 2,3-butanedione
0.087
4-nitrobenzaldehyde
-
NADPH, detection spectrophotometrically
0.0012 - 0.007
5alpha-androstan-3,17-dione
0.00065 - 0.0031
5alpha-androstane-3,17-dione
0.0006 - 0.026
5alpha-dihydroprogesterone
0.0001 - 0.07103
5alpha-dihydrotestosterone
0.000082
5alpha-pregnan-3,20-dione
-
reduction
0.00016 - 0.0016
5beta-androstan-3,17-dione
0.0012 - 0.007
5beta-androstane-3,17-dione
0.018
5beta-Dihydrocortisol
-
-
0.00051 - 0.0012
5beta-dihydroprogesterone
0.235
5beta-Pregnan-3alpha-ol-20-one
-
cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
0.0024
5beta-pregnane-3,20-dione
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.023
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.021 - 0.038
9,10-phenanthrenequinone
0.003
9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one
-
recombinant enzyme in intact COS-1 cells
0.0015
allopregnanolone
-
pH 7.0, 25°C
0.004 - 0.0422
androstanedione
0.0001 - 0.21
androsterone
0.017 - 1.8
benzenedihydrodiol
0.03 - 0.077
Camphorquinone
-
4-nitrobenzaldehyde, 2,3-heptanedione
0.063
chenodeoxycholic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.004
Dehydrolithocholic acid
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.42
diacetyl
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.00315
dihydrotestosterone
37°C, pH not specified in the publication
0.0061 - 0.037
Fusidic acid
0.038
glycocholic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.0052 - 0.0059
glycolithocholic acid
0.022
isatin
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.068
lithocholic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.0025
oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one
-
isozyme AKR1C3
0.00084 - 0.00213
pregn-4-en-3,20-dione
0.043
taurocholic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.0028 - 0.119
testosterone
additional information
additional information
-
0.017
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
-
NADP+, detection spectrophotometrically
0.019
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
-
NADP+, detection radio- or fluorometrically
0.022
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
-
pH 7.0, 37°C with NADP+
0.042
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
-
pH 7.0, 37°C with NAD+
0.022
1-Acenaphthenol
-
pH 7.4, 25°C, recombinant isozyme AKR1C1 and isozyme AKR1C2
0.07
1-Acenaphthenol
-
pH 7.4, 25°C, recombinant isozyme AKR1C3
0.14
1-Acenaphthenol
-
pH 7.4, 25°C, recombinant isozyme AKR1C4
0.048
1-Indanol
-
pH 7.4, 25°C, recombinant isozyme AKR1C1
0.17
1-Indanol
-
pH 7.4, 25°C, recombinant isozyme AKR1C4
0.94
1-Indanol
-
pH 7.4, 25°C, recombinant isozyme AKR1C2
1.4
1-Indanol
-
pH 7.4, 25°C, recombinant isozyme AKR1C3
0.002 - 0.0024
17beta-hydroxy-5alpha-androstan-3-one
-
-
0.002 - 0.0024
17beta-hydroxy-5alpha-androstan-3-one
-
also in this range: 3alpha,7alpha-dihydroxy-5beta-cholanoyl glycine
0.0007 - 0.00075
17beta-hydroxy-5beta-androstan-3-one
-
testis
0.0012 - 0.0017
17beta-hydroxy-5beta-androstan-3-one
-
also in this range: 5beta-dihydrotestosterone, rat epididymis
0.0012 - 0.0017
17beta-hydroxy-5beta-androstan-3-one
-
also in this range: 17alpha,21-dihydroxy-5beta-pregnan-3,11,20-trione, acenaphthequinone
0.0012
5alpha-androstan-3,17-dione
wild-type, pH 7.5, 25°C
0.0012
5alpha-androstan-3,17-dione
wild type enzyme, in 0.1 M HEPES at pH 7.5
0.0022
5alpha-androstan-3,17-dione
mutant N86A, pH 7.5, 25°C
0.0031
5alpha-androstan-3,17-dione
mutant enzyme S114A, in 0.1 M HEPES at pH 7.5
0.005
5alpha-androstan-3,17-dione
mutant Y155F, pH 7.5, 25°C
0.007
5alpha-androstan-3,17-dione
mutant K159A, pH 7.5, 25°C
0.00065
5alpha-androstane-3,17-dione
-
pH 7.0, 25, reduction reaction
0.0012
5alpha-androstane-3,17-dione
wild-type, pH 7.5, 25°C
0.0031
5alpha-androstane-3,17-dione
mutant S114A, pH 7.5, 25°C
0.0006
5alpha-dihydroprogesterone
-
pH 7.0, 25°C, isozyme AKR1C2
0.0011
5alpha-dihydroprogesterone
-
pH 7.0, 25°C, isozyme AKR1C1
0.0018
5alpha-dihydroprogesterone
-
pH 7.0, 25°C
0.026
5alpha-dihydroprogesterone
-
isozyme AKR1C3
0.0001
5alpha-dihydrotestosterone
-
-
0.00062 - 0.00065
5alpha-dihydrotestosterone
-
-
0.0013
5alpha-dihydrotestosterone
-
recombinant enzyme in intact COS-1 cells
0.00218
5alpha-dihydrotestosterone
pH 7.4, 37°C, recombinant wild-type enzyme
0.0029
5alpha-dihydrotestosterone
-
pH 7.0, 25°C, recombinant enzyme
0.003415
5alpha-dihydrotestosterone
37°C, pH 7.2
0.006
5alpha-dihydrotestosterone
-
NADP+, detection radiometrically
0.007 - 0.0083
5alpha-dihydrotestosterone
-
-
0.007 - 0.0083
5alpha-dihydrotestosterone
-
also in this range: androstanedione
0.007 - 0.0083
5alpha-dihydrotestosterone
-
also in this range: 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine
0.007 - 0.0083
5alpha-dihydrotestosterone
-
also in this range: 3alpha-hydroxy-5alpha-androstan-17-one
0.007 - 0.0083
5alpha-dihydrotestosterone
-
also in this range: 3alpha-hydroxy-5beta-pregnan-17-one
0.012
5alpha-dihydrotestosterone
-
-
0.018
5alpha-dihydrotestosterone
-
NADPH, detection radiometrically
0.019
5alpha-dihydrotestosterone
-
NADPH, detection spectrophotometrically
0.0223
5alpha-dihydrotestosterone
-
-
0.026
5alpha-dihydrotestosterone
-
pH 7.0, 37°C, with NAD(P)H
0.07103
5alpha-dihydrotestosterone
pH 7.4, 37°C, recombinant mutant V54L
0.00016
5beta-androstan-3,17-dione
-
-
0.00035 - 0.00049
5beta-androstan-3,17-dione
-
also in this range: 5beta-pregnan-3,20-dione, 5alpha-androstan-3,17-dione
0.0016
5beta-androstan-3,17-dione
-
-
0.0012
5beta-androstane-3,17-dione
wild-type, pH 7.5, 25°C
0.0021
5beta-androstane-3,17-dione
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.0022
5beta-androstane-3,17-dione
mutant N86A, pH 7.5, 25°C
0.005
5beta-androstane-3,17-dione
mutant Y155F, pH 7.5, 25°C
0.007
5beta-androstane-3,17-dione
mutant K159A, pH 7.5, 25°C
0.00051
5beta-dihydroprogesterone
-
pH 7.0, 37°C
0.0007
5beta-dihydroprogesterone
-
pH 7.0, 25°C, isozyme AKR1C2
0.0012
5beta-dihydroprogesterone
-
pH 7.0, 25°C, isozyme AKR1C1
0.021
9,10-phenanthrenequinone
-
NADPH, detection spectrophotometrically
0.038
9,10-phenanthrenequinone
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.004
androstanedione
-
NADPH, detection spectrophotometrically
0.0422
androstanedione
-
-
0.0001
androsterone
-
-
0.00044
androsterone
-
pH 9.0, 25°C, recombinant His-tagged mutant S114A/Y155F
0.0035
androsterone
-
pH 7.4, 25°C, recombinant isozyme AKR1C2 and isozyme AKR1C4
0.0041
androsterone
-
pH 7.0, 25, oxidation reaction
0.0051
androsterone
pH 10.4, 25°C, recombinant wild-type enzyme
0.0055
androsterone
-
pH 9.0, 25°C, recombinant His-tagged wild-type
0.007
androsterone
-
pH 9.0, 25°C, recombinant His-tagged mutant S114A
0.033
androsterone
pH 10.4, 25°C, recombinant mutant K159A
0.048
androsterone
-
NADP+, detection radiometrically
0.065
androsterone
-
pH 9.0, 25°C, recombinant His-tagged mutant K159A
0.074
androsterone
-
pH 9.0, 25°C, recombinant His-tagged mutant Y155F
0.1
androsterone
-
pH 9.0, 25°C, recombinant His-tagged mutant Y155F/K159A
0.21
androsterone
-
pH 8.5, 37°C, with cofactor NAD+
0.017
benzenedihydrodiol
-
pH 7.4, 25°C, recombinant isozyme AKR1C4
0.15
benzenedihydrodiol
-
pH 7.4, 25°C, recombinant isozyme AKR1C1
0.32
benzenedihydrodiol
-
pH 7.4, 25°C, recombinant isozyme AKR1C3
1.8
benzenedihydrodiol
-
pH 7.4, 25°C, recombinant isozyme AKR1C2
0.015
cholate
-
-
56
cholate
37°C, pH 8.0, wild-type enzyme
78
cholate
37°C, pH 8.0, mutant enzyme S114A/Y153F
96
cholate
37°C, pH 8.0, mutant enzyme K157A
468
cholate
37°C, pH 8.0, mutant enzyme Y153F
586
cholate
37°C, pH 8.0, mutant enzyme S114A
0.01 - 0.025
cholic acid
-
-
0.01 - 0.025
cholic acid
-
3alpha-hydroxy-5beta-androstan-17-one, 3alpha,21-dihydroxy-5beta-pregnan-20-one, 5beta-androstan-3alpha,17beta-diol, 5alpha-androstan-3alpha,17beta-diol, NADP+
0.01 - 0.025
cholic acid
-
NAD+, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine
0.031
cholic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.084
cholic acid
-
pH 8.5, 37°C, with cofactor thio-NAD+
51
dehydrocholate
37°C, pH 8.0, wild-type enzyme
239
dehydrocholate
37°C, pH 8.0, mutant enzyme S114A
2489
dehydrocholate
37°C, pH 8.0, mutant enzyme Y153F
0.0061
Fusidic acid
-
-
0.0062
Fusidic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.037
Fusidic acid
-
pH 8.5, 37°C, with cofactor thio-NAD+
0.0052 - 0.0059
glycolithocholic acid
-
-
0.0052 - 0.0059
glycolithocholic acid
-
also in this range: 3alpha-hydroxy-5alpha-pregnan-20-one
0.00305
NAD+
mutant D249S, pH 10.2, 25°C
0.0038
NAD+
wild type enzyme, in 0.1 M CAPS buffer, at pH 10.2, at 25°C
0.0038
NAD+
wild-type, pH 10.2, 25°C
0.005
NAD+
mutant enzyme D249S, in 0.1 M CAPS buffer, at pH 10.2, at 25°C
0.009
NAD+
mutant enzyme D249K, in 0.1 M CAPS buffer, at pH 10.2, at 25°C
0.009
NAD+
mutant D249K, pH 10.2, 25°C
0.016
NAD+
mutant enzyme D249A, in 0.1 M CAPS buffer, at pH 10.2, at 25°C
0.016
NAD+
mutant D249A, pH 10.2, 25°C
0.004
NADH
wild-type, pH 7.5, 25°C
0.004
NADH
wild type enzyme, in 0.1 M HEPES at pH 7.5
0.0065
NADH
mutant enzyme S114A, in 0.1 M HEPES at pH 7.5
0.0065
NADH
mutant S114A, pH 7.5, 25°C
0.01
NADH
mutant N86A, pH 7.5, 25°C
0.029
NADH
mutant Y155F, pH 7.5, 25°C
0.4
NADH
-
cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
0.00023
NADP+
-
pH 7.0, 25°C
0.00007
NADPH
-
pH 7.0, 25°C
0.91
NADPH
-
cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
1.33
NADPH
-
microsomal NADPH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
0.00084
pregn-4-en-3,20-dione
pH 7.4, 37°C, recombinant wild-type enzyme
0.00213
pregn-4-en-3,20-dione
pH 7.4, 37°C, recombinant mutant V54L
0.0028
testosterone
-
-
0.012
testosterone
-
NADP+, detection radiometrically
0.119
testosterone
-
pH 7.4, 35°C, 3alpha-hydroxysteroid dehydrogenase activity
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
values for various substrates and different isozymes
-
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
steady-state kinetics
-
additional information
additional information
-
cofactor binding kinetics
-
additional information
additional information
-
kinetic mechanism, substrate binding mechanism of isozyme AKR1C2
-
additional information
additional information
-
kinetic mechanism, substrate binding mechanism of isozyme AKR1C9, wild-type and mutant enzymes
-
additional information
additional information
-
kinetic modeling of an enzymatic cycling reaction
-
additional information
additional information
steady state kinetics, overview
-
additional information
additional information
-
steady state kinetics, overview
-
additional information
additional information
-
steady-state kinetics, ternary complex, sequential, kinetic mechanism of allopregnanolone synthesis with the cofactor binding before the steroid and leaving after it
-
additional information
additional information
-
equilibrium kinetics of wild-type and mutant enzymes
-
additional information
additional information
-
transient single and multiple turnover kinetics, stopped flow kinetics, isotope kinetics, ligand binding analysis, kinetics with deuterium-substituted cofactor and binary complex of enzyme and cofactor, overview
-
additional information
additional information
-
cofactor kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
kinetics in absence and presence of co-solvents, overview
-
additional information
additional information
kinetics of wild-type and mutant enzymes in presence or absence of CAPS and methylamine, overview
-
additional information
additional information
-
steady-state, transient state kinetics, and kinetic isotope effects, kinetic analysis and mechanism, ordered bi-bi mechanism, detailed overview
-
additional information
additional information
steady-state kinetics, and stopped-flow study, kinetics of enzyme mutants P185A, P185G, T188A, and T188S showing an increase in kcat, Ka drosterone and KiNAD and equal primary isotope effects of DV and D(V/K)
-