1.14.11.33: DNA oxidative demethylase
This is an abbreviated version!
For detailed information about DNA oxidative demethylase, go to the full flat file.
Word Map on EC 1.14.11.33
-
1.14.11.33
-
demethylation
-
3-methylcytosine
-
wobble
-
1,n6-ethenoadenine
-
alkbhs
-
2-oxoglutarate-dependent
-
dealkylation
-
writer
-
etheno
-
epitranscriptomic
-
5-methoxycarbonylmethyluridine
-
n1-methyladenosine
-
n6-methyladenine
-
analysis
- 1.14.11.33
-
demethylation
- 3-methylcytosine
-
wobble
- 1,n6-ethenoadenine
-
alkbhs
-
2-oxoglutarate-dependent
-
dealkylation
-
writer
-
etheno
-
epitranscriptomic
-
5-methoxycarbonylmethyluridine
- n1-methyladenosine
- n6-methyladenine
- analysis
Reaction
Synonyms
1-methyladenine-DNA dioxygenase, ABH1, ABH2, ABH3, AlkB, AlkB homolog 1, ALKBH2, ALKBH3, ALKBH8, alkylated DNA repair protein, alpha-ketoglutarate-dependent dioxygenase ABH1, FTO
ECTree
Advanced search results
General Information
General Information on EC 1.14.11.33 - DNA oxidative demethylase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
malfunction
physiological function
additional information
-
Escherichia coli alkB mutants are defective in processing methylation damage generated in single-stranded DNA
malfunction
-
ABH2 knockdown impairs rDNA transcription and leads to increased single-stranded and double-stranded DNA breaks that are more pronounced in the rDNA genes, whereas ABH2 overexpression protects cells from methyl-methanesulfonate-induced DNA damage and inhibition of rDNA transcription
malfunction
-
an AlkB-deficient strain accumulates N1-methyladenine at a high rate
malfunction
-
an AlkB-deficient strain accumulates N1-methyladenine at a high rate
-
-
ABH3 restores the biological function of mRNA and tRNA inactivated by chemical methylation. A methylation-induced block in translation of an mRNA can be readily relieved by treatment with ABH3 prior to translation. Chemical methylation of tRNA-Phe inhibits aminoacylation and translation, but the inhibition can be reversed by ABH3
physiological function
-
AlkB corrects some of the unwanted methylations of DNA bases by a unique oxidative demethylation in which the methyl carbon is liberated as formaldehyde. The enzyme also repairs exocyclic DNA lesions
physiological function
-
AlkB corrects some of the unwanted methylations of DNA bases by a unique oxidative demethylation in which the methyl carbon is liberated as formaldehyde. The enzyme also repairs exocyclic DNA lesions
physiological function
-
AlkB restores the biological function of mRNA and tRNA inactivated by chemical methylation. A methylation-induced block in translation of an mRNA can be readily relieved by treatment with AlkB prior to translation. Chemical methylation of tRNA-Phe inhibits aminoacylation and translation, but the inhibition can be reversed by AlkB
physiological function
-
AlkB-mediated oxidative demethylation reverses DNA damage
physiological function
isoform ABH2 repairs DNA close to the replication forks, is more active on double-stranded than on single-stranded DNA and is present in different subnuclear compartments at different stages of the cell cycle
physiological function
-
oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage
physiological function
-
the alkB gene product protects against cell killing by SN2-alkylating agents, probably through DNA repair
physiological function
-
the AlkB protein catalyzes the direct reversal of alkylation damage to DNA, primarily 1-methyladenine and 3-methylcytosine lesions created by endogenous or environmental alkylating agents
physiological function
the nuclear localization of ABH3 and the clear preference for single-stranded DNA and RNA suggest that ABH3 has a role in maintenance of nuclear single-stranded DNA and RNA, with genes undergoing transcription potentially being its main targets
physiological function
-
viral AlkB proteins maintain the integrity of the viral RNA genome through repair of deleterious methylation damage
physiological function
-
viral AlkB proteins maintain the integrity of the viral RNA genome through repair of deleterious methylation damage
physiological function
-
viral AlkB proteins maintain the integrity of the viral RNA genome through repair of deleterious methylation damage
physiological function
-
critical role of ABH2 in maintaining rDNA gene integrity and transcription, the DNA alkylation repair enzyme ABH2/ALKBH2 is highly enriched in the nucleolus. ABH2 overexpression protects cells from methyl-methanesulfonate-induced DNA damage and inhibition of rDNA transcription. In response to massive alkylation damage, ABH2 rapidly redistributes from the nucleolus to nucleoplasm. ABH2 interacts with DNA repair proteins Ku70 and Ku80 as well as nucleolar proteins nucleolin, nucleophosmin 1, and upstream binding factor. ABH2 associates with and promotes rDNA transcription through its DNA repair activity. The physical association between ABH2 and Ku70/Ku80 may help mobilize ABH2 to DSBs to remove alkylation damage and thus facilitate the DNA repair process, or the physical association between ABH2 and Ku70/Ku80 may allow Ku70/Ku80 to sense the potential sites of DSBs through recognition of alkylation damages by ABH2
physiological function
-
protein AlkB repairs cytotoxic 1-methyladenine and 3-methylcytosine lesions induced by methyl methanesulfonate in DNA and RNA in vitro and in pre-damaged DNA and RNA bacteriophages in vivo, quantification of AlkB-mediated repair of endogenous RNA and DNA in vivo, overview
physiological function
-
the DNA and RNA repair protein AlkB removes alkyl groups from nucleic acids by a unique iron- and 2-oxoglutarate-dependent oxidation strategy, role for AlkB in regulation of important cellular functions in vivo. Complete adduct restructuring of ethano-adenine, a DNA adduct formed by an important anticancer drug, BCNU, by AlkB to a form that does not hinder replication. Ethano-adenine dealkylation process requires two oxidation reactions, one at N1 and a second at N6, to achieve complete restoration of the undamaged adenine base. AlkB DNA substrate N6-methyladenine is an important epigenetic signal for DNA replication and many other cellular processes
physiological function
Escherichia coli W3110 / ATCC 27325
-
AlkB-mediated oxidative demethylation reverses DNA damage
-
physiological function
-
protein AlkB repairs cytotoxic 1-methyladenine and 3-methylcytosine lesions induced by methyl methanesulfonate in DNA and RNA in vitro and in pre-damaged DNA and RNA bacteriophages in vivo, quantification of AlkB-mediated repair of endogenous RNA and DNA in vivo, overview
-
-
ABH2 associates with DNA repair proteins Ku70/Ku80, overview
additional information
-
the recombinant AlkB expressed is less active than the natural endogenous form
additional information
-
the recombinant AlkB expressed is less active than the natural endogenous form
-