1.14.13.64: 4-hydroxybenzoate 1-hydroxylase

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For detailed information about 4-hydroxybenzoate 1-hydroxylase, go to the full flat file.

Reaction

Synonyms

4-hydroxybenzoate 1-hydroxylase, 4-hydroxybenzoate 1-hydroxylase (decarboxylating), 4-hydroxybenzoate 1-monooxygenase, 4HB1H, Cp_4HB1H, FAD-dependent 4-hydroxybenzoate hydroxylase, MNX1, Mnx1p, Oxygenase, 4-hydroxybenzoate 1-mono, PHBH, V1H, vanillate 1-hydroxylase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.64 4-hydroxybenzoate 1-hydroxylase

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Results	in table
1	AA Sequence
6	Application
1	CAS Registry Number
3	Cloned(Commentary)
17	Cofactor
10	General Information
7	Inhibitors
3	kcat/KM [mM/s]
18	KM Value [mM]
2	Localization
5	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
34	Organism
2	Pathway
5	pH Optimum
2	Posttranslational Modification
1	Protein Variants
3	Purification (Commentary)
3	Reaction
2	Reaction Type
12	Reference
2	Specific Activity [micromol/min/mg]
70	Substrates and Products (Substrate)
10	Subunits
26	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
17	Turnover Number [1/s]

Engineering

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Engineering on EC 1.14.13.64 - 4-hydroxybenzoate 1-hydroxylase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

additional information

Yarrowia lipolytica

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Yarrowia lipolytica is engineered for enhanced production of arbutin. To construct the arbutin synthetic pathway in Yarrowia lipolytica, three genes, chorismate pyruvate-lyase (UbiC), 4-hydroxybenzoate 1-hydroxylase (MNX1), and hydroquinone glucosyltransferase (AS), are codon-optimized and heterologously expressed in Yarrowia lipolytica strain polf (leucine and uracil auxotroph). To maximize arbutin production, seven arbutin-biosynthesis molecular targets are overexpressed, and individual strengthening of DHS1 and DHS2 leads to an 8.9 and 7.8fold improvement in arbutin yield, respectively. Through optimization, a maximum arbutin titer of 8.6 g/l is achieved using the finally engineered Yarrowia lipolytica strain po1f-At0 Improvement in arbutin yields by glucose feeding

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