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1.14.20.1: deacetoxycephalosporin-C synthase

This is an abbreviated version!
For detailed information about deacetoxycephalosporin-C synthase, go to the full flat file.

Word Map on EC 1.14.20.1

Reaction

Penicillin N
+
2-oxoglutarate
+
O2
=
deacetoxycephalosporin C
+
succinate
+
CO2
+
H2O

Synonyms

acDAOC/DACS, cefE, cefEF, Cephalosporin biosynthesis expandase/hydroxylase, DAOC synthase, DAOC/DAC synthase, DAOC/DACS, DAOCS, deacetoxy/deacetylcephalosporin C synthase, deacetoxycephalosporin C synthase, deacetoxycephalosporin-C synthase, deacetoxycephalosporin-C synthetase, deacetoxycephalosporin/deacetylcephalosporin C synthase, expandase, expendase, penicillin N expandase, scDAOCS

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated
                1.14.20.1 deacetoxycephalosporin-C synthase

Molecular Weight

Molecular Weight on EC 1.14.20.1 - deacetoxycephalosporin-C synthase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
-
gel filtration
28000
-
1 * 28000, SDS-PAGE
28900
29500
-
gel filtration
30966
-
3 * 30966, DAOCS is in equilibrium with a trimeric form which is the form that crystallizes
34500
x * 34500, about, recombinant wild-type and mutant enzymes, SDS-PAGE
34519
34554
x * 34554, in solution the monomeric apoenzyme is in equilibrium with atrimeric form, electrospray mass spectrometry
35000
-
gel filtration
36000
36348
x * 36348, wild-type enzyme, electrospray mass spectrometry
36642
-
x * 40000, SDS-PAGE, x * 36642, calculated
40000
41000
-
1 * 41000, SDS-PAGE
43000
-
gel filtration
60000
62000
x * 60000, recombinant truncated mutant enzyme, SDS-PAGE, x * 62000, recombinant wild-type enzyme
additional information