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1.3.1.32: maleylacetate reductase

This is an abbreviated version!
For detailed information about maleylacetate reductase, go to the full flat file.

Word Map on EC 1.3.1.32

Reaction

3-oxoadipate
+
NAD(P)+
=
2-maleylacetate
 +
NAD(P)H
 +
H+

Synonyms

GraC, hadD, hqdD, HqoD, HxqD, MAA reductase, maleoylacetate reductase, maleylacetate reductase, MAR, PcpE, pdcF, PnpD, TcpD, tfdF, tsdD

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.32 maleylacetate reductase

Storage Stability

Storage Stability on EC 1.3.1.32 - maleylacetate reductase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 100 mM Tris/HCl buffer, pH 7.0, containing 50% (vol/vol) glycerol, 1 mM dithioerythritol and 0.5 mM EDTA, purified enzyme retains 80% of its initial activity after storage for 2 months
-
-20°C, 30% glycerol, 4 months, enzyme retains its activity
-23°C, purified enzyme in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA after freezing, storage overnight and rethawing 35% loss of activity
-
-25°C, 50 mM Tris/H2SO4 buffer, pH 7.6, can be stored for several months without loss of activity
-
-70°C, 30% glycerol, 6 months, enzyme retains its activity
4°C, 30% glycerol, 3 weeks, enzyme retains its activity
4°C, purified enzyme can be stored in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA with slight loss of activity, 36% in 3 weeks
-
5°C, 50 mM Tris/H2SO4 buffer, pH 7.6, can be stored for several days without loss of activity
-