2.1.1.171: 16S rRNA (guanine966-N2)-methyltransferase
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For detailed information about 16S rRNA (guanine966-N2)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.171
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2.1.1.171
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tuberculosis
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mycobacterial
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non-canonical
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chromatin
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histone
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non-cpg
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cytosines
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epigenome
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minimalist
- 2.1.1.171
- tuberculosis
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mycobacterial
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non-canonical
- chromatin
- histone
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non-cpg
- cytosines
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epigenome
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minimalist
Reaction
Synonyms
16S rRNA-specific methyltransferases m2GMT, EC 2.1.1.51, EC 2.1.1.52, m2G966 methyltransferase, RNA:(guanine-N2) methyltransferase RsmD, RsmD, RsmD-like methyltransferase, Rv2966c, Rv2966c protein, small rRNA methyltransferase, yhhF
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2.1.1.171 16S rRNA (guanine966-N2)-methyltransferaseAdvanced search results
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General Information on EC 2.1.1.171 - 16S rRNA (guanine966-N2)-methyltransferase
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GENERAL INFORMATION 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
evolution
structural similarities with other methyltransferases are limited to the core S-adenosyl-L-methionine domain but not the target recognition domain
malfunction
additional information
malfunction
disruption of the yhhF gene by kanamycin resistance marker leads to a loss of modification at guanine966. Doubling of strain JW3430 carrying the inactive rsmD gene is essentially the same as that of the parental Escherichia coli strain BW25141
malfunction
truncated constructs of Rv2966c lacking the N-terminal 18- or 23-amino acid residues and the mutant protein 3RM do not show any significant binding to either DNA or RNA nor any m2G966 methyltransferase activity
malfunction
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enzyme deficiency increases initiation of translation from codons AUA, CAC and CAU (2 to 3.6fold)
malfunction
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enzyme deletion has a subtle effect on maintenance of the reading frame
malfunction
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enzyme deletion has a subtle effect on maintenance of the reading frame
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additional information
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RsmD is unusual in its ability to withstand multiple amino acid substitutions of the active site. Such efficiency of RsmD may be useful to complete the modification of a 30S subunit ahead of the 30S subunit's involvement in translation
additional information
the N-terminal hairpin is a minimalist functional domain that helps Rv2966c in target recognition
additional information
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the N-terminal hairpin is a minimalist functional domain that helps Rv2966c in target recognition
