2.1.1.215: tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase
This is an abbreviated version!
For detailed information about tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase, go to the full flat file.
Reaction
4 S-adenosyl-L-methionine + = 4 S-adenosyl-L-homocysteine +
Synonyms
EC 2.1.1.32, multisite-specific tRNA methyltransferase, Trm1, Trm1[tRNA (m2(2)G26) methyltransferase], tRNA (m2(2)G26) methyltransferase, tRNA (N2,N2-guanine)-dimethyltransferase, tRNA:m22G26-methyltransferase
ECTree
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KM Value
KM Value on EC 2.1.1.215 - tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase
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0.00009
pH 7.5, 55°C, mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27
-
0.00009
guanine26 in mutant tRNATyr
pH 7.5, 55°C, mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27, additional point mutation C43U
-
0.00008
pH 7.5, 55°C, tRNA Tyr with wild-type sequence adenine26/guanine27 and mutation C43U
-
0.00017
guanine27 in tRNATyr
pH 7.5, 55°C, wild-type tRNATyr contains the sequence adenine26/guanine27
-
0.00017
S-adenosyl-L-methionine
-
pH 7.5, 55°C, recombinant wild-type enzyme
0.00067
S-adenosyl-L-methionine
-
pH 7.5, 55°C, recombinant mutants L60A, F140A, and E113A