2.1.1.251: methylated-thiol-coenzyme M methyltransferase
This is an abbreviated version!
For detailed information about methylated-thiol-coenzyme M methyltransferase, go to the full flat file.
Reaction
Synonyms
480-kDa CoM methylase, CoM methylase, DMS:CoM methyltransferase, methylthiol:coenzyme M methyltransferase, methylthiol:CoM methyltransferase, methyltransferase II, MMPA:CoM methyltransferase, MtsA
ECTree
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Renatured
Renatured on EC 2.1.1.251 - methylated-thiol-coenzyme M methyltransferase
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recombinant enzyme from inclusion bodies harvested from cells solubilized using 6 M guanidine hydrochloride, 50 mM DTT, and 20 mM 2-mercaptoethanol. The solubilized protein is diluted 60fold with protein refolding at room temperature for 24 h in refolding buffer consisting of 500 mM Tris, pH 8.0, 1 M KCl, 10 mM DTT, 10 mM 2-mercaptoethanol, and 100 mM ZnCl2. Refolding buffers containing 500 mM guanidine hydrochloride, 33 mM CHAPS, 0.5% Triton X-100, 5 mM SDS, or 20% glycerol do not produce active methyltransferase, nor do refolding at 4°C in the optimal refolding buffer
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