2.1.1.282: tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase

This is an abbreviated version!
For detailed information about tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase, go to the full flat file.

Reaction

Synonyms

aTrm5a methyltransferase, aTrm5a-type enzyme, aTrm5a/Taw22-like enzyme, More, NEQ228, PAB2272, PaTrm5a, SSO2439, Taw21, TAW22, Trm5a, Trm5a/Taw22-like enzyme, tRNAPhe:imG2 methyltransferase, TYW3, YGL050w

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.282 tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase

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Results	in table
2928	AA Sequence
6	Cloned(Commentary)
5	Cofactor
2	Crystallization (Commentary)
38	General Information
5	Metals/Ions
21	Natural Substrates/ Products (Substrates)
12	Organism
1	Pathway
4	pH Optimum
16	Protein Variants
5	Purification (Commentary)
1	Reaction
11	Reference
40	Substrates and Products (Substrate)
3	Subunits
50	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 2.1.1.282 - tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant apo-PaTrm5a and PaTrm5a in complex with tRNAPhe, and PaTrm5a-tRNAPhe (imG-14)-SAH ternary complex, X-ray diffraction structure determination and analysis

Pyrococcus abyssi

Q9V2G1

758348

purified recombinant PaTrm5a in apo form and in complex with various SAM analogues, mixing PaTrm5a with 1.5 mM SAH or SAM at a protein: ligand molar ratio of 1:3, sitting drop vapor diffusion method, mixing 20 mg/ml protein solution in a 1:1 ratio with well solution containing w/v PEG 3350, 100 mM HEPES, pH 7.5, 100 mM Ca(OAc)2 and 100 mM KCl., method optimmization, 25°C, X-ray diffraction structure determination and analysis at 1.76-2.20 A resolution

Pyrococcus abyssi

Q9V2G1

758376