2.1.1.308: cytidylyl-2-hydroxyethylphosphonate methyltransferase
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For detailed information about cytidylyl-2-hydroxyethylphosphonate methyltransferase, go to the full flat file.
Reaction
2 S-adenosyl-L-methionine + + = + + + +
Synonyms
(S)-2-hydroxyethylphosphonate methylase, 2-hydroxyethylphosphonate methyltransferase, fom3, methyltransferase fom3
ECTree
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Cloned
Cloned on EC 2.1.1.308 - cytidylyl-2-hydroxyethylphosphonate methyltransferase
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cloning and characterization of a complete fosfomycin biosynthetic cluster from Streptomyces fradiae and heterologous production of fosfomycin in Streptomyces lividans
overexpression in Escherichia coli Rosetta 2 (DE3) pLysS cells as a hexahistidine-tagged protein. The recombinant proteins are found exclusively in inclusion bodies. After refolding and concentration, the protein is reconstituted with additional iron and sulfide to maximize cluster formation