2.1.1.314: diphthine methyl ester synthase
This is an abbreviated version!
For detailed information about diphthine methyl ester synthase, go to the full flat file.
Reaction
4 S-adenosyl-L-methionine + = 4 S-adenosyl-L-homocysteine +
Synonyms
diphthine methyltransferase, diphthine synthase, Dph5, S-adenosyl-L-methionine:elongation factor 2 methyltransferase
ECTree
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Natural Substrates Products
Natural Substrates Products on EC 2.1.1.314 - diphthine methyl ester synthase
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REACTION DIAGRAM
4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2]
4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]
4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2]
4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]
Drosophila sp. (in: flies)
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post-translational modification of eukaryotic elongation factor 2 (eEF2)
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4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2]
4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]
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4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2]
4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]
the eukaryotic enzyme is part of the biosynthetic pathway of diphthamide. The modification is sufficient to create diphtheria toxin sensitivity
diphthine methyl ester i.e. 2-[(3S)-3-carboxy methyl ester-3-(trimethylammonio)propyl]-L-histidine
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?
4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2]
4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]
eukaryotic diphthine synthase, Dph5, is a promiscuous methyltransferase that catalyzes an extraordinary N,O-tetramethylation of 2-(3-carboxy-3-aminopropyl)-L-histidine to yield diphthine methyl ester. This compound is an intermediates in the biosynthesis of the post-translationally modified histidine residue diphthamide, a unique and essential residue part of the eukaryotic elongation factor 2 (eEF2)
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4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2]
4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]
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in eukaryotes, the modification of an invariant histidine (His-699 in yeast) residue in translation elongation factor 2 (EF2) with diphthamide involves a conserved pathway encoded by the DPH1-DPH7 gene network. Diphthamide is the target for diphtheria toxin and related lethal ADP ribosylases, which collectively kill cells by inactivating the essential translocase function of EF2 during mRNA translation and protein biosynthesis
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4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2]
4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]
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the enzyme is involved in the diphthamide modification pathway
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?
4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2]
4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]
the eukaryotic enzyme is part of the biosynthetic pathway of diphthamide. The modification is sufficient to create diphtheria toxin sensitivity
diphthine methyl ester i.e. 2-[(3S)-3-carboxy methyl ester-3-(trimethylammonio)propyl]-L-histidine
-
?