2.1.1.74: methylenetetrahydrofolate-tRNA-(uracil54-C5)-methyltransferase [NAD(P)H-oxidizing]

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For detailed information about methylenetetrahydrofolate-tRNA-(uracil54-C5)-methyltransferase [NAD(P)H-oxidizing], go to the full flat file.

Reaction

Synonyms

EC 2.1.2.12, FDRTS, folate-dependent ribothymidyl synthase, folate-dependent tRNA methyltransferase, folate/FAD-dependent tRNA T54 methyltransferase, methylenetetrahydrofolate-transfer ribonucleate uracil 5-methyltransferase,, TRMFO, tRNA (m5U54)-methyltransferase, tRNA:m5U-54 MTase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.74 methylenetetrahydrofolate-tRNA-(uracil54-C5)-methyltransferase [NAD(P)H-oxidizing]

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Results	in table
11291	AA Sequence
8	Application
1	CAS Registry Number
5	Cloned(Commentary)
19	Cofactor
2	Crystallization (Commentary)
1	General Information
1	General Stability
11	KM Value [mM]
1	Localization
2	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
25	Organism
3	PDB ID
5	pH Optimum
17	Protein Variants
6	Purification (Commentary)
2	Reaction
2	Reaction Type
25	Reference
2	Specific Activity [micromol/min/mg]
45	Substrates and Products (Substrate)
33	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]

Purification

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Purification on EC 2.1.1.74 - methylenetetrahydrofolate-tRNA-(uracil54-C5)-methyltransferase [NAD(P)H-oxidizing]

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Enterococcus faecalis

-

485582, 485583

purified to homogeneity by metal affinity or ion exchange and heparin affinity, respectively, followed by gel filtration

Bacillus subtilis

-

720967

recombinant enzyme from Escherichia coli strain BL21(DE3) by anion and cation exchange chromatography and gel filtration to over 95% purity

Thermotoga maritima

-

701488

recombinant enzyme from Escherichia coli to homogeneity

Thermotoga maritima

Q9WZJ3

706541

using Ni-NTA chromatography

Bacillus subtilis

-

718903