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abaI_Km mutant
mutant failed to produce any detectable acyl-homoserine lactone signals
T140A
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site-directed mutagenesis of EsaI, the mutant shows altered substrate acyl-chain length specificity compared to the wild-type enzyme
T142A
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site-directed mutagenesis of LasI, the mutant shows slightly altered substrate acyl-chain length specificity compared to the wild-type enzyme
T142G
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site-directed mutagenesis of LasI, the mutant shows reduced activity and altered substrate acyl-chain length specificity compared to the wild-type enzyme
T142S
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site-directed mutagenesis of LasI, the mutant shows slightly altered substrate acyl-chain length specificity compared to the wild-type enzyme
T144V
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site-directed mutagenesis of LasI, the mutant shows reduced activity and altered substrate acyl-chain length specificity compared to the wild-type enzyme
F69L
site-directed mutagenesis of ExpISCC1, the mutant shows altered substrate acyl-chain lemgth specificity compared to the wild-type enzyme
M127T
site-directed mutagenesis of ExpISCC1, the mutant shows altered substrate acyl-chain lemgth specificity compared to the wild-type enzyme
C126S
site-directed mutagenesis, the mutant shows 81.5% activity compared to the wild-type enzyme
C67S
site-directed mutagenesis, the mutant shows 1.9% activity compared to the wild-type enzyme
C67S/C69S
site-directed mutagenesis, the mutant shows 0.8% activity compared to the wild-type enzyme
C69S
site-directed mutagenesis, the mutant shows 30.4% activity compared to the wild-type enzyme
C89S
site-directed mutagenesis, the mutant shows 63% activity compared to the wild-type enzyme
D48N
site-directed mutagenesis, inactive mutant
D51N
site-directed mutagenesis, nearly inactive mutant
E101K
site-directed mutagenesis, inactive mutant
E144K
site-directed mutagenesis, the mutant shows 43.5% activity compared to the wild-type enzyme
E46K
site-directed mutagenesis, inactive mutant
E7K/F147L/P159E/E182G
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strain R2: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type and yield a hexanoyl homoserine lactone level comparable to the butanoyl homoserine lactone concentration
E7K/F147L/V201M
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strain R1: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type
F28L
site-directed mutagenesis, the mutant shows 0.18% activity compared to the wild-type enzyme
G159E
site-directed mutagenesis, the mutant shows 44.6% activity compared to the wild-type enzyme
G68D
site-directed mutagenesis, the mutant shows 0.075% activity compared to the wild-type enzyme
G68E
site-directed mutagenesis, inactive mutant
K150E/R154QE
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site-directed mutagenesis, the mutant shows highly reduced activity compared to the fully active mutant LasIDELTAG
K150Q
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site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG
K150Q/R154Q
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site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG
R104C
site-directed mutagenesis, nearly inactive mutant
R104H
site-directed mutagenesis, nearly inactive mutant
R154E
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site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG
R154Q
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site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG
R172A
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site-directed mutagenesis, the mutant shows increased activity compared to the fully active mutant LasIDELTAG
R24W
site-directed mutagenesis, inactive mutant
R71C
site-directed mutagenesis, the mutant shows 0.05% activity compared to the wild-type enzyme
R71H
site-directed mutagenesis, inactive mutant
S103E
site-directed mutagenesis, the mutant shows 5.4% activity compared to the wild-type enzyme
W34G
site-directed mutagenesis, the mutant shows 0.10% activity compared to the wild-type enzyme
W34Y
site-directed mutagenesis, the mutant shows 60% activity compared to the wild-type enzyme
additional information
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inactivation of abaI gene in the chromosome of the Acinetobacter spp. S117 by insertion disruption, using the middle along with the flanking chromosomal DNA cloned into pUC18-based suicide delivery vector from Escherichia coli K12 carrying the pUC18-abaI::Tc plasmid, introduction of the plasmid by conjugation
additional information
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btaI1 and btaI3 gene disruption mutant strains cause hyper-hemolysis of sheep erythrocytes
additional information
knockout mutants of strain EC1 are created via transposons
additional information
strains with in frame deletions of mrlI1 and mrlI2 are produced
additional information
strains with in frame deletions of mrlI1 and mrlI2 are produced
additional information
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strains with in frame deletions of mrlI1 and mrlI2 are produced
additional information
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strains with in frame deletions of mrlI1 and mrlI2 are produced
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additional information
several quorum-sensing autoinducer-deficient mutants of Mesorhizobium tianshanense are produced by using the mariner transposon-carrying vector pSC1376
additional information
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construction of the mutant strain WP38 lacking pgaI and showing no AHL activity and reduced cell growth compared to the wild-type
additional information
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construction of the mutant strain WP38 lacking pgaI and showing no AHL activity and reduced cell growth compared to the wild-type
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additional information
screening of diverse mutants constructrd by random mutagenesis, overview
additional information
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screening of diverse mutants constructrd by random mutagenesis, overview
additional information
strains lacking RhlI/R or LasI/R are created
additional information
strains lacking RhlI/R or LasI/R are created
additional information
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strains lacking RhlI/R or LasI/R are created
additional information
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strains lacking RhlI/R or LasI/R are created
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