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2.3.1.225: protein S-acyltransferase

This is an abbreviated version!
For detailed information about protein S-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.225

Reaction

palmitoyl-CoA
+
[protein]-L-cysteine
=
[protein]-S-palmitoyl-L-cysteine
 +
CoA

Synonyms

Akr1, Akr1p, APT1, Asp-His-His-Cys motif palmitoyl transferase, At3g51390, DHHC palmitoyl transferase, DHHC protein, DHHC protein acyltransferase, DHHC-2, DHHC-21, DHHC-21 palmitoyl transferase, DHHC-3, DHHC-3 palmitoyl transferase, DHHC-7, DHHC-8, DHHC-CRD S-acyltransferase, DHHC16, DHHC17, DHHC2, DHHC3, DHHC4, DHHC5, Erf2, Erf2p, G-protein palmitoyltransferase, HIP14, palmitoyl acyltransferase, Pat, PAT10, PAT14, PAT15, PAT21, PAT24, PAT4, Pfa3, Pfa4, PFA5, protein acyl transferase, protein acyltransferase, protein S-acyl transferase 4, Ras PAT, S-protein acyltransferase, SEC18p, Swf1, ZDHHC3, ZDHHC5

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.225 protein S-acyltransferase

Purification

Purification on EC 2.3.1.225 - protein S-acyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Akr1p is purified from yeast to apparent homogeneity
-
native enzyme 120fold by anion exchange and hydroxylapatite chromatography
native enzyme from brain membranes partially by gel filtration and anion exchange chromatography
-
partially, from placental membranes by anion exchange chromatography
-
recombinant FLAG- and His6-tagged DHHC2 from Sf9 cells by nickel and FLAG affinity chromatography to near homogeneity
-
recombinant FLAG- and His6-tagged wild-type and C157S mutant DHHC3 from Sf9 cells by nickel and FLAG affinity chromatography to near homogeneity