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2.3.1.42: glycerone-phosphate O-acyltransferase

This is an abbreviated version!
For detailed information about glycerone-phosphate O-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.42

Reaction

acyl-CoA
+
glycerone phosphate
=
CoA
 +
acylglycerone phosphate

Synonyms

acyltransferase, dihydroxyacetone phosphate, DAP-AT, DAT, DHAP acyltransferase, DHAP-AT, DHAPAT, dihydroxyacetone phosphate acyl-transferase, dihydroxyacetone phosphate acyltransferase, dihydroxyacetone-phosphate acyltransferase, dihydroxyacetonephosphate acyltransferase, glyceronephosphate O-acyltransferase, GNPAT, LmDAT, male sterility protein, TtFARAT

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.42 glycerone-phosphate O-acyltransferase

Cloned

Cloned on EC 2.3.1.42 - glycerone-phosphate O-acyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme cloned from a cDNA library matching EST-clones with peptides from rabbit peroxisomal enzyme, DNA sequence determination
expressed in HEK-293E cells and Pichia pastoris KM71H MutS strain
expressed in Saccharomyces cerevisiae cells
-
expression in CHO cell line NRe1-4 with severe enzyme activity defects restores the plasmalogen biosynthesis activity to 10% of wild-type activity, overexpression of the human enzyme leads to 6fold increased enzyme activity and 55% restored plasmalogen synthesizing activity, nonether glycerolipid biosynthesis is unaffected or dcreased in both cases
-
expression of the human enzyme encoding cDNA in a CHO cell line NRe1-4 with severe enzyme activity defects restores the plasmalogen biosynthesis activity to 10% of wild-type activity, overexpression of the human enzyme leads to 6fold increased enzyme activity and 55% restored plasmalogen synthesizing activity, nonether glycerolipid biosynthesis is unaffected or decreased in both cases
-
functional expression in Saccharomyces cerevisiae
functional expression in Saccharomyces cerevisiae, DNA and amino acid sequence determination
-
gene DAT, DNA amd amino acid sequence determination and analysis, DAT functionally complements the lethality resulting from the loss of both dihydroxyacetone phosphate and glycerol-3-phosphate acyltransferase activities in yeast, recombinant DAT exhibits biochemical properties similar to those of the native enzyme of the promastigote stage parasites
-
gene TTHERM_00221020, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant transient expression of GFP-tagged truncated bifunctional enzyme in Nicotiana tabacum cv. Petit Havana epidermis, using the Agrobacterium tumefaciens GV3101 strain and used for transient expression system, and transgenic expression in Saccharomyces cerevisiae, the recombinant enzyme's N-terminal FAR-like domain produces both 16:0 and 18:0 fatty alcohols, whereas the C-terminal acyltransferase-like domain is able to rescue the lethal phenotype of the Saccharomyces cerevisiae double mutant cmy228 (gat1DELTAgat2DELTA). Coexpression in Saccharomyces cerevisiae with the alkyl-dihydroxyacetone phosphate synthase from Tetrahymena thermophila results the detection of various glycerolipids with an ether bond. In yeast, GAT1 and GAT2 are the only two acyltransferases acylating the sn-1 position of G3P and DHAP, thus being essential to initiate glycerolipid biosynthesis
genes GAT1 and GAT2, expression in Escherichia coli DH5alpha, Gat1 and Gat2 proteins are glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferases, amino acid sequence analysis
-
PCR-amplification, transfection of Leishmania by electroporation
-