2.4.1.14: sucrose-phosphate synthase
This is an abbreviated version!
For detailed information about sucrose-phosphate synthase, go to the full flat file.
Word Map on EC 2.4.1.14
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2.4.1.14
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- 2.4.1.14
-
student
-
skill
- starch
-
social
-
anxiety
-
sintering
-
spark
-
post-traumatic
- invertase
-
stiff
-
physician
-
polystyrene
-
traumatic
-
school
-
senile
-
fear
-
emotional
-
session
-
services
-
faculty
-
tangles
-
microstructure
-
satisfaction
-
serrated
-
neurofibrillary
-
interpersonal
-
portray
-
undergraduate
-
colonoscopy
-
learner
-
anti-gad
-
curriculum
-
alloy
-
ceramics
-
raters
-
videotape
-
phobia
-
thematic
- hyperkalemia
-
transcranial
-
cronbach
-
trainees
-
empathy
-
patient-centered
-
self-assessment
-
postgraduate
-
examinees
-
first-year
-
sanitary
-
problem-solving
- biofuel production
- agriculture
- diagnostics
- analysis
Reaction
Synonyms
At5g20280, AtSPS, More, SPS, SPS A, SPS1, SPS11, Sps2, SPS4, SPS6, SPS8, SPSA, SpsB, SPSII, sucrose 6-phosphate synthase, sucrose phosphate synthase, sucrose phosphate synthase 4, sucrose phosphate synthase A, sucrose phosphate synthase B, sucrose phosphate synthetase, sucrose phosphate-uridine diphosphate glucosyltransferase, sucrose-P synthase, sucrose-phosphate synthase B, sucrosephosphate-UDP glucosyltransferase, UDP-glucose-fructose-phosphate glucosyltransferase, UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase, uridine diphosphoglucose-fructose phosphate glucosyltransferase
ECTree
Advanced search results
Engineering
Engineering on EC 2.4.1.14 - sucrose-phosphate synthase
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D498A
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the mutant shows strongly decreased activity compared to the wild type enzyme
P497A
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the mutant shows slightly increased activity compared to the wild type enzyme
S495A
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the mutant shows about 2fold increased activity compared to the wild type enzyme
additional information
construction of multiple knockout mutants of the four Arabidopsis sucrose phosphate synthase isozymes SPSA1, SPSA2, SPSB, and SPSC (T-DNA insertion mutants spsa1 (SALK 119162), spsa2 (SALK 064922), spsb (GABI 368F01) and spsc (SAIL 31 H05) and mutants spsa1/spsa2, spsa1/spsb, spsa1/spsc, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsb/spsc, spsa1/spsa2/spsb, spsa1/spsa2/spsc, spsa2/spsb/spsc, and spsa1/spsa2/spsb/spsc). Despite their reduced SPS activity, mutants spsa1/spsa2, spsa1/spsb, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsa2/spsb and spsa2/spsb/spsc display wild-type vegetative and reproductive morphology, and show wild-type photosynthetic capacity and respiration, phenotypes overview. Growth of rosettes, flowers and siliques of the spsa1/spsc and spsa1/spsa2/spsc mutants is reduced compared with wild-type plants. The plants display a high dark respiration phenotype. Mutant spsa1/spsb/spsc and spsa1/spsa2/spsb/spsc seeds poorly germinate and produce aberrant and sterile plants. Leaves of all viable sps mutants, except spsa1/spsc and spsa1/spsa2/spsc, accumulate wild-type levels of nonstructural carbohydrates. Mutant spsa1/spsc leaves possess high levels of metabolic intermediates and activities of enzymes of the glycolytic and tricarboxylic acid cycle pathways, and accumulate high levels of metabolic intermediates of the nocturnal starch-to-sucrose conversion process, even under continuous light conditions
additional information
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enzyme downregulation by gene SPS1 antisense expression in transgenic plants using the CMV 35S promoter and Agrobacterium tumefaciens LBA4404 transfection. The transgenic plants show a phenotype with reduces sucrose phosphate synthase activity, fruit size and sucrose concentration, affected muskmelon leaf subcellular structure in SPS1 antisense muskmelon, overview
additional information
overexpression of the enzyme from Zea mays in Medicago sativa, gene SPS-1 or SPSA expression is driven by the constitutive CaMV35S promoter. Overexpression and increased activity of the enzyme in alfalfa is accompanied by early flowering, increased plant growth and an increase in elemental N and protein content when grown under N2-fixing conditions, phenotype, overview
additional information
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overexpression of the enzyme from Zea mays in Medicago sativa, gene SPS-1 or SPSA expression is driven by the constitutive CaMV35S promoter. Overexpression and increased activity of the enzyme in alfalfa is accompanied by early flowering, increased plant growth and an increase in elemental N and protein content when grown under N2-fixing conditions, phenotype, overview
additional information
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expression of the SPS gene from Arabidosis thaliana in Populus alba x Populus grandidentata causes an altered phenotype compared to the wild-type trees with altered timing of bud flush and leaf senescence. Tree height and stem diameter are similar to the wild-type, but differences in the length of xylem fibres occur. Elevated concentrations of intracellular sucrose in both leaf and stem tissue of the transgenic trees are associated with a prolonged onset of senescence and an advancement in bud flush in the following spring, phenotypes, overview
additional information
construction of N-terminally truncated enzymeslacking up to 171 amino acid residues in the 20 kDa N-terminal region, the mutants cannot be activated
additional information
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construction of N-terminally truncated enzymeslacking up to 171 amino acid residues in the 20 kDa N-terminal region, the mutants cannot be activated
additional information
production of transgenic tobacco plants with a cytosolic soybean glutamine synthetase (GS1) gene and a sucrose phosphate synthase (SPS) gene from maize, both driven by the CaMV35S promoter. The SPS enzyme activity in the SPS and SPS/GS1 co-transformants are the same under both nitrogen regimens, low and high nitrogen. The GS1/SPS transformants show a phenotype similar to the SPS transformants, phenotype overview
additional information
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production of transgenic tobacco plants with a cytosolic soybean glutamine synthetase (GS1) gene and a sucrose phosphate synthase (SPS) gene from maize, both driven by the CaMV35S promoter. The SPS enzyme activity in the SPS and SPS/GS1 co-transformants are the same under both nitrogen regimens, low and high nitrogen. The GS1/SPS transformants show a phenotype similar to the SPS transformants, phenotype overview