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2.4.1.301: 2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase

This is an abbreviated version!
For detailed information about 2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase, go to the full flat file.

Reaction

UDP-alpha-D-kanosamine
+
2'-deamino-2'-hydroxyneamine
=
UDP
+
kanamycin A

Synonyms

KanE, KanM2

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.301 2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase

Cloned

Cloned on EC 2.4.1.301 - 2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
expression in Streptomyces venezuelae. Reconstructed the kanamycin pathway stepwise in a mutant strain of Streptomyces venezuelae lacking the genes for biosynthesis of the native deoxysugar by transplanting plasmids harboring different combinations of putative genes from Streptomyces kanamyceticus in Streptomyces venezuelae and then carrying out biochemical analysis and chemical characterization