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2.5.1.55: 3-deoxy-8-phosphooctulonate synthase

This is an abbreviated version!
For detailed information about 3-deoxy-8-phosphooctulonate synthase, go to the full flat file.

Word Map on EC 2.5.1.55

Reaction

phosphoenolpyruvate
+
D-arabinose 5-phosphate
 +
H2O
=
3-deoxy-D-manno-octulosonate 8-phosphate
 +
phosphate

Synonyms

2-dehydro-3-deoxy-D-octonate-8-phosphate D-arabinose-5-phosphate-lyase (pyruvate-phosphorylating), 2-dehydro-3-deoxyphosphooctonate aldolase, 2-keto-3-deoxy-8-phosphooctonic acid synthetase, 2-keto-3-deoxy-8-phosphooctonic synthetase, 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase, 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase, 3-deoxy-D-manno-2-octulosonic acid-8-phosphate synthase, 3-deoxy-D-manno-octulosonate 8-phosphate synthase, 3-deoxy-D-manno-octulosonate 8-phosphate synthetase, 3-deoxy-D-manno-octulosonate-8-phosphate synthase, 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase, 3-deoxy-D-mannooctulosonate-8-phosphate synthetase, 3-deoxyoctulosonic 8-phosphate synthetase, 3-eoxy-D-manno-octulosonate 8-phosphate synthase, 8-phospho-2-dehydro-3-deoxy-D-octonate D-arabinose-5-phosphate-lyase (pyruvate-phosphorylating), aldolase, phospho-2-keto-3-deoxyoctonate, AtkdsA1, AtkdsA2, EC 4.1.2.16, HpKDO8PS, KDO 8-phosphate synthetase, KDO-8-P synthase, KDO-8-P synthetase, Kdo-8-phosphate synthase, KDO8-P, Kdo8P synthase, KDO8PS, KDOPS, KDPO synthetase, KdsA, KdsA1, KdsA2, metal-independent 3-deoxy-D-manno-octulosonate 8-phosphate synthase, metal-independent KDO8PS, NmeKDO8PS, Phospho-2-dehydro-3-deoxyoctonate aldolase, phospho-2-keto-3-deoxyoctonate aldolase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.55 3-deoxy-8-phosphooctulonate synthase

Cloned

Cloned on EC 2.5.1.55 - 3-deoxy-8-phosphooctulonate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
1 15.5-kb segment containing the kdsA gene, expression in Escherichia coli
-
cloned into a T7-driven expression vector, overexpression in Escherichia coli
-
enzyme from wild-type strain AG701 and temperature-sensitive strain AG701i50 expressed in Escherichia coli
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Salmonella enterica serovar typhimurium
expression in Salmonella enterica serovar typhimurium AG701i50
-
expression in Salmonella typhimurium
-
gene kdo8ps, recombinant expression of His6-tagged codon-optimized enzyme in Escherichia coli strain Rosetta2 (pLysS) DE3
-
gene kdsA, recombinant expression of His-tagged enzyme in Escherichia coli strain pTf16/BL21
gene kdsA1, in Arabidopsis a functionally redundant gene pair encodes Kdo-8-P synthases, AtKdsA1 (At1g9500) and AtKdsA2 (At1g16340), recombinant expression in Escherichia coli
gene kdsA1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene kdsA2, in Arabidopsis a functionally redundant gene pair encodes Kdo-8-P synthases, AtKdsA1 (At1g9500) and AtKdsA2 (At1g16340), recombinant expression in Escherichia coli
gene kdsA2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)
in Escherichia coli with plasmid pet28a
localization of the kdsA gene with the aid of the physical map of the Escherichia coli chromosome
-