2.5.1.73: O-phospho-L-seryl-tRNA:Cys-tRNA synthase
This is an abbreviated version!
For detailed information about O-phospho-L-seryl-tRNA:Cys-tRNA synthase, go to the full flat file.
Word Map on EC 2.5.1.73
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2.5.1.73
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cys-trnacys
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methanogen
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archaea
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o-phosphoseryl-trna
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seprs
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cysteinyl-trna
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o-phosphoserine
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sulfur
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cysrs
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trna-dependent
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cysteinyl-trnacys
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jannaschii
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methanocaldococcus
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selenocysteine
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pyridoxal
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methanosarcina
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sec
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aminoacylation
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misacylated
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acetivorans
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auxotrophy
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persulfide
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relay
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sep-trna:sec-trna
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homocysteine
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fulgidus
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isoacceptors
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aminoacyl-trnas
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archaeoglobus
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trna-bound
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maripaludis
- 2.5.1.73
-
cys-trnacys
-
methanogen
- archaea
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o-phosphoseryl-trna
- seprs
-
cysteinyl-trna
- o-phosphoserine
- sulfur
- cysrs
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trna-dependent
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cysteinyl-trnacys
- jannaschii
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methanocaldococcus
- selenocysteine
- pyridoxal
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methanosarcina
-
sec
- aminoacylation
-
misacylated
- acetivorans
-
auxotrophy
- persulfide
-
relay
-
sep-trna:sec-trna
- homocysteine
- fulgidus
-
isoacceptors
- aminoacyl-trnas
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archaeoglobus
-
trna-bound
- maripaludis
Reaction
Synonyms
MJ1678, Sep-tRNA-Cys-tRNA synthase, Sep-tRNA:Cys-tRNA synthase, SepCysS
ECTree
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Crystallization
Crystallization on EC 2.5.1.73 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase
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purified recombinant wild-type SepVysS1 and selenomethionine-labeled SepCysS1, hanging-drop vapor diffusion method, 0.001 ml protein solution is mixed with 0.001 ml reservoir solution containing 80 mM sodium acetate buffer, pH 4.4, 160 mM NaCl, and 1.00 M ammonium sulfate, 20°C, equilibration against 0.5 ml reservoir solution, cryoprotection by 25% v/v glycerol, X-ray diffraction structure determination and analysis at 2.4-3.2 A resolution, modeling
crystal structure of a subcomplex of the transsulfursome with tRNACys (SepCysS-SepCysE-tRNACys), which is involved in the second reaction step of the indirect pathway, to 2.6 A resolution. Space group P6522, with unit-cell parameters a = b = 107.2, c = 551.1 A. Two heterodimers of a synthase SepCysS dimer, an enhancer SepCysE dimer and one tRNACys are observed in the asymmetric unit
crystal structure of the complex of the enzyme with translation factor SepCysE, 2.8 A resolution
structure of the SepCysE-SepCysS-tRNACys ternary complex, at a resolution of 2.6 A by molecular replacement. SepCysS recognizes the discriminator base U73 of tRNACys