2.6.1.9: histidinol-phosphate transaminase
This is an abbreviated version!
For detailed information about histidinol-phosphate transaminase, go to the full flat file.
Word Map on EC 2.6.1.9
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2.6.1.9
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aminotransferases
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5'-phosphate
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auxotrophic
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transamination
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aldimine
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plp-dependent
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pyridoxamine
- 2.6.1.9
- aminotransferases
- 5'-phosphate
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auxotrophic
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transamination
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aldimine
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plp-dependent
- pyridoxamine
Reaction
Synonyms
aminotransferase, histidinol phosphate, AtHPA1, glutamic-imidazoleacetol phosphate transaminase, HisC, HisC2, histidine:imidazoleacetol phosphate transaminase, histidinol phosphate aminotransferase, histidinol-phosphate aminotransferase, HspAT, IAP transaminase, imidazoleacetol phosphate transaminase, imidazolylacetolphosphate aminotransferase, imidazolylacetolphosphate transaminase, L-histidinol phosphate aminotransferase, Rv1600
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Purification
Purification on EC 2.6.1.9 - histidinol-phosphate transaminase
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from derepressed histidine auxotrophs, 95% pure
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purified to homogeneity using nickel-nitrilotriacetic acid metal-affinity and gel filtration chromatography
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recombinant wild-type and selenomethionine-labeled enzyme from Escherichia coli strain BL21(DE3) and B834(DE3), respectively
use of omega-aminoalkylagaroses, 15fold purification in one step
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