2.7.1.136: macrolide 2'-kinase

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For detailed information about macrolide 2'-kinase, go to the full flat file.

Reaction

Synonyms

macrolide 2'-phosphotransferase, macrolide 2'-phosphotransferase II, macrolide 2'-phosphotransferase type I, macrolide 2'-phosphotransferase type II, Mph, MPH (2'), MPH(2'), MPH(2')-I, MPH(2')-II, mphA, mphB, phosphotransferase, macrolide 2'-

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.136 macrolide 2'-kinase

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Results	in table
1	Activating Compound
7	AA Sequence
1	Application
1	CAS Registry Number
8	Cloned(Commentary)
2	Cofactor
2	Crystallization (Commentary)
4	General Information
11	Inhibitors
1	kcat/KM [mM/s]
1	Ki Value [mM]
1	KM Value [mM]
2	Localization
10	Metals/Ions
1	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
34	Organism
1	pH Optimum
6	Protein Variants
1	Purification (Commentary)
1	Reaction
1	Reaction Type
24	Reference
12	Specific Activity [micromol/min/mg]
1	Storage Stability
97	Substrates and Products (Substrate)
1	Subunits
20	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.7.1.136 - macrolide 2'-kinase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified isozyme MPH(2')-I in apoform and in complex with GTP analogues and six different macrolides (guanosine 5'-[beta,gamma-imido]triphosphate (GMPPNP) or guanosine diphosphate (GDP) and either a 14-membered lactone macrolide: erythromycin, oleandomycin, or clarithromycin, or the 15-membered lactone macrolide azithromycin), X-ray diffraction structure determination analysis at 1.2-1.6 A resolution, molecular replacement method, modeling. Despite the inclusion of MgCl2 in the crystallization experiments, no evidence of magnesium ions is observed in any of the MPH(2')-I electron density maps

Escherichia coli

O32553, Q9EVJ6

762485

purified isozyme MPH(2')-II in apoform and in complex with GTP analogues and six different macrolides (guanosine diphosphate (GDP) or GDP or GTPgS (guanosine 5'-O-[gamma-thio]triphosphate) and either a 14-membered lactone macrolide: erythromycin, oleandomycin, or clarithromycin, or the 15-membered lactone macrolide azithromycin), X-ray diffraction structure determination analysis at 1.31-1.65 A resolution, molecular replacement method, modeling. At least one divalent metal ion (either Mg2+ or Ca2+, based on the geometry and bond distances) from the crystallization solution is observed in the nucleotide binding pocket

Escherichia coli

O32553, Q9EVJ6

762485