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2.7.1.15: ribokinase

This is an abbreviated version!
For detailed information about ribokinase, go to the full flat file.

Word Map on EC 2.7.1.15

Reaction

ATP
+
D-ribose
=
ADP
+
D-ribose 5-phosphate

Synonyms

At1g17160, D-ribokinase, deoxyribokinase, Ms4585, rbk, RBSK, rbsK1, ribokinase, ribokinase (phosphorylating), Rv2436, Sa239

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.15 ribokinase

Crystallization

Crystallization on EC 2.7.1.15 - ribokinase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
20°C or 16°C, hanging drop method, 7 mg/ml protein mixed with an equal volume of mother liquor
4°C, hanging drop vapour diffusion, Cs-bound ternary complex structure: P212121
ribokinase in complex with ribose and dinucleotide: there is one 33000 Da monomer of ribokinase in the asymmetric unit but the protein forms a dimer around a crystallographic twofold axis
-
several different types of crystals, apo ribokinase: space group P1 or P3x21, ATP ribokinase: tetragonal, ribose/AMP-PNP: P6x22
-
crystals are obtained using the sitting-drop method. Diffraction data are collected to a resolution of 3.1 A
crystals obtained by the hanging-drop vapour-diffusion method at 23°C diffract to 1.95 A resolution and belong to the hexagonal space group P61, with unit-cell parameters a = b = 100.25, c = 126.77 A
hanging drop vapour diffusion method, the crystal structure is determined in the apo and complex forms with several nucleotides (ATP, AMPPCP and ADP) in the presence of Na+ ion
by using the hanging-drop vapour-diffusion method, large crystals appear at 21.85°C in conditions consisting of 23% PEG 3000, 0.1 M sodium citrate pH 5.6, the crystals diffract to 2.9 A resolution and belong to space group P6122 or P6522, with unit-cell parameters a=b=91.8 A, c=160.7 A
-
crystal structure of Sa239 in the absence of monovalent ions. The structure of Sa239 demonstrates that the C-terminal tail protrudes from the remaining part and interacts with the neighboring molecule, resulting in an dimerization form
capillary counter-diffusion technique, 2.87 A resolution
in complex with ribose and ADP, to 3.4 and 1.75 A resolution, respectively. Both crystals possess symmetry consistent with space group P1