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2.7.1.163: hygromycin B 4-O-kinase

This is an abbreviated version!
For detailed information about hygromycin B 4-O-kinase, go to the full flat file.

Word Map on EC 2.7.1.163

Reaction

ATP
+
hygromycin B
=
ADP
 +
4-O-phosphohygromycin B

Synonyms

Hm phosphotransferase, HPH, Hph5, HPT, hptII, hygromycin B phosphotransferase, hygromycin B4-O-phosphohygromycin, hygromycin phosphotransferase, hygromycin-B kinase, hygromycin-phosphotransferase, HyR

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.163 hygromycin B 4-O-kinase

Crystallization

Crystallization on EC 2.7.1.163 - hygromycin B 4-O-kinase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour-diffusion method method. The crystals provide diffraction data to a resolution of 2.1 A and belong to space group P3(2)21, with unit-cell parameters a = b = 71.0 A, c = 125.0 A. Crystals of complexes of Hph with hygromycin B and AMP-PNP or ADP shows the same crystal form as that of the apoprotein
-
using a thermostable mutant and the hanging-drop vapour-diffusion method at 20°C. Thermostable proteins crystallize with less difficulty than wild-type proteins
-