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2.7.7.2: FAD synthase

This is an abbreviated version!
For detailed information about FAD synthase, go to the full flat file.

Word Map on EC 2.7.7.2

Reaction

ATP
+
FMN
=
diphosphate
 +
FAD

Synonyms

adenosine triphosphate-riboflavin mononucleotide transadenylase, adenosine triphosphate-riboflavine mononucleotide transadenylase, ATP-FMN adenylyltransferase, ATP:FMN adenylyl transferase, ATP:FMN adenylyltransferase, AtRibF1, AtRibF2, FAD pyrophosphorylase, FAD synthase, FAD synthetase, FAD synthetase isoform 1, FAD synthetase isoform 2, Fad1, FADS, FADS-6, FADS1, FADS2, FLAD1, flavin adenine dinucleotide synthetase, FMN adenylyltransferase, FMN pyrophosphorylase, FMN:ATP adenylyltransferase, FMNAT, lysZ, MJ1179, More, ribF, RibL, riboflavin adenine dinucleotide pyrophosphorylase, riboflavin mononucleotide adenylyltransferase, riboflavine adenine dinucleotide adenylyltransferase, SPAP_1083

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.2 FAD synthase

Purification

Purification on EC 2.7.7.2 - FAD synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, Phenyl-Sepharose chromatography, DEAE-Cellulose chromatography
-
by ion exchange chromatography and gel filtration
by Ni-NTA affinity chromatography, ion exchange chromatography and gel filtration
DEAE-cellulose column chromatography, phenyl Sepharose column chromatography, and Sephacryl S-200 gel filtration
Mono Q column chromatography
Ni-Sepharose affinity chromatography, anion exchange chromatography, hydrophobic interaction chromatography, gel filtration
Ni2+-chelating column chromatography and hydroxyapatite column chromatography
Ni2+-chelating Sepharose Fast Flow column chromatography
Ni2+-nitrilotriacetic-agarose column chromatography
-
partially by subcellular fractionation and nucleus preparation
phenyl Sepharose column chromatography and DEAE-cellulose column chromatography
-
recombinant C-terminally poly-His-tagged N-terminally truncated mutant_187-338 from Escherichia coli by ion exchange chromatography and gel filtration
recombinant enzyme
-
recombinant protein
recombinant RFK module of enzyme FADS, DELTA(1-182)CaFADS, from Escherichia coli strain BL21(DE3)
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction and ion exchange chromatography, followed by dialysis
wild-type and recombinant enzyme