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2.7.7.30: fucose-1-phosphate guanylyltransferase

This is an abbreviated version!
For detailed information about fucose-1-phosphate guanylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.30

Reaction

GTP
+
beta-L-fucose 1-phosphate
=
diphosphate
+
GDP-L-fucose

Synonyms

AtFKGP, FKP, GDP-fucose pyrophosphorylase, GDP-L-Fuc pyrophosphorylase, GDP-L-fucose pyrophosphorylase, GDP-L-fucose pyrophosphorylase (Fpgt), GDP-L-fucose-synthesizing enzymes, GDPfucose pyrophosphorylase, GFPP, GTP fucose pyrophosphorylase, GTP fucose pyrophosphorylase (GFPP), guanosine 5'-diphosphate-L-fucose pyrophosphorylase, guanosine diphosphate L-fucose pyrophosphorylase, guanosine triphosphate fucose pyrophosphorylase (GFPP), L-fucokinase/GDP-fucose pyrophosphorylase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.30 fucose-1-phosphate guanylyltransferase

Storage Stability

Storage Stability on EC 2.7.7.30 - fucose-1-phosphate guanylyltransferase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-16°C, 10 mM dithioerythritol, 24 hours, 30-35% loss of activity
-
-16°C, 24 hours, 80% loss of activity
-
-20°C, 50 mM sucrose, several months, no loss of activity
-
-20°C, 50% (w/v) glycerol, 1 mM PMSF, 0.2 M NaCl, 50 mM Tris-HCl buffer, pH 7.5, 1 month, no loss of activity
-
-20°C, recombinant protein with maltose-binding protein affinity tag, stable for at least three months
0°C, 24 hours, 85% loss of activity
-
4°C, 1mM PMSF, 0.2 M NaCl, 50 mM Tris-HCl buffer, pH 7.5, 1 day, loss of more than 50% activity
-