Information on EC 1.1.1.71 - alcohol dehydrogenase [NAD(P)+]

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.1.1.71
-
RECOMMENDED NAME
GeneOntology No.
alcohol dehydrogenase [NAD(P)+]
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a primary alcohol + NAD(P)+ = an aldehyde + NAD(P)H + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
alcohol:NAD(P)+ oxidoreductase
Reduces aliphatic aldehydes of carbon chain length from 2 to 14, with greatest activity on C4, C6 and C8 aldehydes; also reduces retinal to retinol.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-10-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strains NCIB 8250 and HO1-N
-
-
Manually annotated by BRENDA team
strain NCIMB 3610
-
-
Manually annotated by BRENDA team
Bacillus subtilis NCIMB 3610
strain NCIMB 3610
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
strain ML30
-
-
Manually annotated by BRENDA team
strain ML30
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain IFO 3426
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-
Manually annotated by BRENDA team
strain IFO 3426
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
var. bosis
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-
Manually annotated by BRENDA team
strains NCIMB 10548 and 196A
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-
Manually annotated by BRENDA team
NCIMB 9494
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-
Manually annotated by BRENDA team
NCIMB 9494
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-
Manually annotated by BRENDA team
strain NCIMB 13259
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-
Manually annotated by BRENDA team
strain NCIMB 13259
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-
Manually annotated by BRENDA team
strain KGX 39
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-
Manually annotated by BRENDA team
strain KGX 39
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-
Manually annotated by BRENDA team
D273-10B
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-
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
strain 4018
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-
Manually annotated by BRENDA team
strain 4018
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S,5S)-2,5-hexanediol + NADP+
? + NADPH + H+
show the reaction diagram
(S)-2-butanol + NADP+
butanone + NADPH
show the reaction diagram
-
196% relative activity as compared to 2-propanol
-
-
?
(S)-2-butanol + NADP+
butanone + NADPH + H+
show the reaction diagram
-
196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol
-
-
?
1,3-butanediol + NADP+
? + NADPH + H+
show the reaction diagram
-
91% relative activity as compared to 2-propanol
-
-
?
1-butanol + NADP+
1-butanal + NADPH + H+
show the reaction diagram
-
-
-
?
1-butanol + NADP+
butanal + NADPH
show the reaction diagram
-
80% relative activity as compared to 2-propanol
-
-
?
1-butanol + NADP+
butanal + NADPH + H+
show the reaction diagram
1-pentanol + NADP+
pentanal + NADPH + H+
show the reaction diagram
1-phenylethanol + NADP+
1-phenylethanone + NADPH + H+
show the reaction diagram
35% of the activtiy with 1-propanol
-
-
?
1-phenylethanol + NADP+
acetophenone + NADPH + H+
show the reaction diagram
35% compared to activity with 1-propanol
-
-
?
1-phenylmethanol + NADP+
benzaldehyde + NADPH + H+
show the reaction diagram
-
180% relative activity as compared to 2-propanol
-
-
?
1-propanol + NAD+
propanal + NADH + H+
show the reaction diagram
dual cofactor dependency, NAD+ shows 60% of the activity with NADP+
-
-
?
1-propanol + NADP+
propanal + NADPH + H+
show the reaction diagram
2,3-pentanedione + NADPH + H+
? + NADP+
show the reaction diagram
2-butanol + NADP+
2-butanone + NADPH + H+
show the reaction diagram
43% of the activity compared to ethanol
-
-
-
2-butanol + NADP+
butanone + NADPH + H+
show the reaction diagram
2-hydroxy-3-methoxybenzaldehyde + NADPH + H+
2-hydroxy-3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
?
2-methoxybenzaldehyde + NADPH + H+
2-methoxybenzalcohol + NADP+
show the reaction diagram
highest activity
-
-
?
2-pentanol + NADP+
2-pentanone + NADPH + H+
show the reaction diagram
-
67% relative activity as compared to 2-propanol
-
-
?
2-pentanol + NADP+
pentan-2-one + NADPH + H+
show the reaction diagram
-
67% of the activity with 2-propanol
-
-
?
2-propanol + NADP+
acetone + NADPH
show the reaction diagram
2-propanol + NADP+
acetone + NADPH + H+
show the reaction diagram
3,4-dimethoxybenzaldehyde + NADPH + H+
3,4-dimethoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
?
3-methyl-2-pentanone + NADPH + H+
? + NADP+
show the reaction diagram
-
13% relative activity as compared to pyruvaldehyde
-
-
?
3-methylbutanal + NADP+
? + NADPH + H+
show the reaction diagram
about% of the activity with 1-butanol
-
-
?
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzalcohol + NADP+
show the reaction diagram
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
show the reaction diagram
all-trans-retinol + NADP+
all-trans-retinal + NADPH + H+
show the reaction diagram
-
-
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r
allyl alcohol + NADP+
acrylaldehyde + NADPH
show the reaction diagram
benzaldehyde + NAD(P)H
benzyl alcohol + NAD(P)+
show the reaction diagram
-
-
-
?
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
show the reaction diagram
-
-
-
?
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
-
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
show the reaction diagram
butan-1,4-diol + NADP+
butandialdehyde + NADPH
show the reaction diagram
-
29% of activity with hexan-1-ol
-
r
butan-1-ol + NADP+
butanal + NADPH
show the reaction diagram
butanal + NAD(P)H + H+
butan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
cellobiose + NADP+
? + NADPH + H+
show the reaction diagram
cinnamaldehyde + NADPH + H+
cinnamyl alcohol + NADP+
show the reaction diagram
-
-
-
?
cis-2-butenol + NAD(P)H
trans-2-butenal + NAD(P)+
show the reaction diagram
cis-4-heptenal + NAD(P)+
cis-4-heptenol + NAD(P)H
show the reaction diagram
-
-
-
?
cis-hex-3-en-1-ol + NADP+
cis-hex-3-en-1-al + NADPH
show the reaction diagram
-
18.7% of activity with hexan-1ol
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r
citral + NAD(P)H
citrol + NAD(P)+
show the reaction diagram
-
-
-
?
D-arabinose + NADP+
? + NADPH + H+
show the reaction diagram
-
200% relative activity as compared to 2-propanol
-
-
?
D-arabinose + NADP+
D-arabitol + NADPH + H+
show the reaction diagram
-
200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose
-
-
?
D-glucose + NADP+
? + NADPH + H+
show the reaction diagram
D-mannose + NADP+
? + NADPH + H+
show the reaction diagram
-
48% relative activity as compared to 2-propanol
-
-
?
D-ribose + NADP+
? + NADPH + H+
show the reaction diagram
-
35% relative activity as compared to 2-propanol
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?
D-xylose + NADP+
? + NADPH + H+
show the reaction diagram
decan-1-ol + NADP+
decanal + NADPH
show the reaction diagram
-
decrease in enzyme activity with increase in chain length
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r
decanal + NAD(P)H
decan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
dimethylglyoxal + NADPH + H+
? + NADP+
show the reaction diagram
dodecan-1-ol + NADP+
dodecanal + NADPH
show the reaction diagram
-
decrease in enzyme activity with increase in chain length
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r
dodecanal + NAD(P)H
dodecan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
ethanal + NAD(P)H
ethanol + NAD(P)+
show the reaction diagram
-
-
-
?
ethanediol + NADP+
glyoxal + NADPH
show the reaction diagram
ethanol + NAD+
acetaldehyde + NADH + H+
show the reaction diagram
enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+
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-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
show the reaction diagram
ethanol + NADP+
ethanal + NADPH + H+
show the reaction diagram
farnesyl aldehyde + NAD(P)H
farnesol + NAD(P)+
show the reaction diagram
-
-
-
?
glycerol + NADP+
glyceraldehyde + NADPH + H+
show the reaction diagram
-
16% relative activity as compared to 2-propanol
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-
?
glyoxylic acid + NADPH + H+
? + NADP+
show the reaction diagram
-
36% relative activity as compared to pyruvaldehyde
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?
heptan-1-ol + NADP+
heptanal + NADPH
show the reaction diagram
-
-
-
r
heptanal + NAD(P)H
heptan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
hexan-1-ol + NADP+
hexanal + NADPH
show the reaction diagram
hexanal + NADPH + H+
hexanol + NADP+
show the reaction diagram
-
-
-
?
hydrocinnamaldehyde + NADPH + H+
hydrocinnamyl alcohol + NADP+
show the reaction diagram
-
-
-
?
isoamyl alcohol + NADP+
? + NADPH + H+
show the reaction diagram
about 80% of the activity with 1-butanol
-
-
?
L-arabinose + NADP+
? + NADPH + H+
show the reaction diagram
-
17% relative activity as compared to 2-propanol
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-
?
linolenyl aldehyde + NAD(P)H
linolenol + NAD(P)+
show the reaction diagram
-
-
-
?
linoleyl aldehyde + NAD(P)H
linoleol + NAD(P)+
show the reaction diagram
-
-
-
?
mannitol + NADP+
D-mannose + NADPH
show the reaction diagram
-
6.5% of activity with hexan-1-ol
-
r
methylglyoxal + NADPH
2-hydroxy-propanal + NADPH
show the reaction diagram
-
-
-
?
octan-1-ol + NADP+
octanal + NADPH
show the reaction diagram
-
decrease in enzyme activity with increase in chain length
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r
octanal + NAD(P)H + H+
octan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
octanal + NADPH + H+
octanol + NADP+
show the reaction diagram
-
-
-
?
oleyl aldehyde + NAD(P)H
oleol + NAD(P)+
show the reaction diagram
-
-
-
?
pentan-1-ol + NADP+
pentanal + NADPH
show the reaction diagram
propan-1-ol + NADP+
propanal + NADPH
show the reaction diagram
pyruvaldehyde + NADPH + H+
? + NADP+
show the reaction diagram
-
-
-
-
?
pyruvaldehyde + NADPH + H+
lactaldehyde + NADP+
show the reaction diagram
retinal + NAD(P)H
retinol + NAD(P)+
show the reaction diagram
-
-
-
?
retinaldehyde + NADH
retinol + NAD+
show the reaction diagram
ribitol + NADP+
D-ribose + NADPH
show the reaction diagram
-
4% of activity with hexan-1-ol
-
r
salicylaldehye + NADPH + H+
salicylalcohol + NADP+
show the reaction diagram
-
-
-
?
tetradecanal + NAD(P)H
tetradecan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
trans-2,cis-6-nonadienal + NAD(P)+
trans-2,cis-6-nonadienol + NAD(P)H
show the reaction diagram
-
-
-
?
trans-2,cis-6-nonadienal + NADPH + H+
trans-2,cis-6-nonadienol + NADP+
show the reaction diagram
-
-
-
?
trans-2-hexenal + NADPH + H+
trans-2-hexenol + NADP+
show the reaction diagram
-
-
-
?
trans-2-nonenal + NADPH
trans-2-nonen-1-ol + NADPH
show the reaction diagram
-
-
-
?
trans-2-nonenal + NADPH + H+
trans-2-nonenol + NADP+
show the reaction diagram
-
-
-
?
trans-2-octenal + NADPH
trans-2-octen-1-ol + NADPH
show the reaction diagram
-
-
-
?
trans-2-pentenol + NAD(P)+
trans-2-pentenal + NAD(P)H
show the reaction diagram
-
-
-
?
trans-trans-2,4-decadienal + NADPH
trans-trans-2,4-decadienol + NADPH
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
retinaldehyde + NADH
retinol + NAD+
show the reaction diagram
-
the enzyme is involved in retinol and vitamin A metabolism, retinoic acid is important in intestinal epithelial cell proliferation and differentiation, the enzyme also plays a role in dietary conversion of beta-carotene to retinol via retinaldehyde
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-
r
additional information
?
-
Q8H0L8
the enzyme plays an important role in potato defence response to Erwinia carotovora
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD(P)H
NADP+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
optimally active in the presence of 2 M KCl
additional information
-
metal independency is supported by the absence of a significant effect of TsAdh319 preincubation with 10 mM Me2+ for 30 min before measuring the activity in the presence of 1 mM Me2+ or EDTA
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
slight inhibition
4-chloromercuribenzoate
8-hydroxyquinoline
-
1 mM, 100% inhibition with NAD+ as cofactor, 40% with NADP+ as cofactor
acetonitrile
-
complete inhibition in buffer without NaCl and in buffer with 600 mM NaCl
AgNO3
-
1 mM, 100% inhibition
CdCl2
-
1 mM, 24% inhibition with NAD+ as cofactor
Chloroform
-
21% inhibition in buffer without NaCl, 19% inhibition in buffer with 600 mM NaCl
CuSO4
-
1 mM, 75% inhibition with NAD+ as cofactor, 56% with NADP+ as cofactor
Dimethyl formamide
-
87% inhibition in buffer without NaCl, 59% inhibition in buffer with 600 mM NaCl
dimethyl sulfoxide
-
complete inhibition in buffer without NaCl, 60% inhibition in buffer with 600 mM NaCl
ethyl acetate
-
complete inhibition in buffer without NaCl, 67% inhibition in buffer with 600 mM NaCl
Fe2+
-
10 mM, 74% of initial activity
FeSO4
HgCl2
-
1 mM, 100% inhibition
iodoacetamide
-
1 mM, complete inhibition
iodoacetic acid
-
1 mM, 29% inhibition with NAD+ as cofactor, 80% with NADP+ as cofactor
methanol
-
75% inhibition in buffer without NaCl, 81% inhibition in buffer with 600 mM NaCl
Mg2+
-
10 mM, 78% of initial activity
n-decane
-
9% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl
N-ethylmaleimide
n-hexane
-
40% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl
o-phenanthroline
p-hydroxymercuribenzoate
-
strong inhibition
Phenobarbital
-
0.5 mM, 30% inhibition
pyrazole
-
0.5 mM, 35% inhibition
Sodium azide
-
10 mM, 40% inhibition
Sodium glycocholate
-
-
Tween 80
-
-
Zn2+
-
10 mM, 79% of initial activity
ZnSO4
-
10 mM, 21% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cysteine
-
-
methyl jasmonate
-
salicylic acid
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13.1
(2S,5S)-2,5-hexanediol
-
60C, pH 10.5
-
6.7
1-butanol
pH 10.0, temperature not specified in the publication
21.4
1-propanol
1.5
2,3-Pentanedione
-
60C, pH 7.5
4.8
2-Methoxybenzaldehyde
pH 6.5, 30C
168
2-propanol
24.1
4-methoxybenzaldehyde
pH 6.5, 30C
1.3 - 9.2
acetaldehyde
0.08
all-trans-retinal
pH 7.4, 37C
0.4
all-trans-retinol
pH 7.4, 37C
150
benzyl alcohol
-
-
52.7 - 78.6
butan-1-ol
0.006 - 3.97
Butanal
54.4
D-xylose
-
60C, pH 10.5; pH 10.5, 60C
44 - 4790
ethanol
1.09
heptan-1-ol
-
-
1.22 - 6.8
hexan-1-ol
5.5
Hexanal
pH 6.5, 30C
0.0107
methylglyoxal
-
-
0.5 - 1060
NAD+
0.024 - 1200
NADH
0.02 - 1
NADP+
0.00079 - 0.32
NADPH
6.2
octan-1-ol
-
-
5.8 - 14.1
pentan-1-ol
17.75
Pyruvaldehyde
6.9
Salicylaldehyde
pH 6.5, 30C
27.1
trans-2-hexenal
pH 6.5, 30C
0.0066
trans-2-nonenal
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.2
(2S,5S)-2,5-hexanediol
Thermococcus sibiricus
-
60C, pH 10.5
-
1.4
2,3-Pentanedione
Thermococcus sibiricus
-
60C, pH 7.5
0.53
2-propanol
38.3 - 50.6
butan-1-ol
19.6 - 26.3
Butanal
0.7
D-xylose
Thermococcus sibiricus
-
60C, pH 10.5; pH 10.5, 60C
13.9 - 35.1
ethanol
38.9
heptan-1-ol
Acinetobacter calcoaceticus
-
-
39.4 - 40.3
hexan-1-ol
0.45 - 2.02
NADP+
1.51
NADPH
25.4 - 39.8
octan-1-ol
44.7 - 62.9
pentan-1-ol
2.04
Pyruvaldehyde
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
(2S,5S)-2,5-hexanediol
Thermococcus sibiricus
-
60C, pH 10.5
194816
0.479
1-butanol
Haloferax volcanii
D4GP73
pH 10.0, temperature not specified in the publication
556
1.33
2,3-Pentanedione
Thermococcus sibiricus
-
60C, pH 7.5
1963
0.003 - 0.0032
2-propanol
682
0.744
acetaldehyde
Haloferax volcanii
D4GP73
pH 6.0, temperature not specified in the publication
90
0.013
D-xylose
Thermococcus sibiricus
-
60C, pH 10.5
115
0.07
ethanol
Haloferax volcanii
D4GP73
pH 10.0, temperature not specified in the publication
69
12.39 - 101
NADP+
10
75.5 - 242.8
NADPH
5
0.11
Pyruvaldehyde
Thermococcus sibiricus
-
60C, pH 7.5
5610
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
-
aldehyde reduction
6.3
-
reduction of retinal, sharp decrease above pH 6.3 and below pH 6.0
6.5
-
strain B593, butanal reduction
7.4
-
assay at
8.6
-
cofactor NADP+
9.5
-
ethanol oxidation, cofactor NAD+
10.5
-
assay at, oxidation of alcohols
10.7
-
hexan-1-ol oxidation
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
-
aldehyde reduction, cofactor NADH, NADP dependent activity occurs only at pHs below 7
6 - 9
-
strain B592
6.4 - 7.15
7 - 10.5
-
ethanol oxidation, cofactor NAD+
9
-
strain B592, gradual increase up to pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
55 - 60
oxidation of alcohol
70
-
oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl
83
-
oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 80
40C: about 50% of maximal activity, 80C: about 70% of maximal activity
50 - 75
-
50C: about 50% of maximal activity, 75C: about 60% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl
65 - 86
-
65C: about 50% of maximal activity, 86C: about 50% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl
70 - 95
70C: 60% of maximal activity, 95C: 70% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
SDS-PAGE
56000 - 60000
-
gel filtration
132000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2.15 A resolution, tetramer of 222 symmetry, the monomer is composed of a cofactor-binding domain and a catalytic domain, it contains a single zinc atom in the putative active side, the tetramer is composed of two dimers
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2.05 A resolution, tetramer of 222 symmetry, the monomer is composed of a cofactor-binding domain and a catalytic domain, it contains a single zinc atom in the putative active side, the tetramer is composed of two dimers
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hanging-drop vapour-diffusion method using 25% (w/v) polyethylene glycol 3350 pH 7.5 as precipitant; to 1.68 A resolution, crystals belong to space group I222, with unit-cell parameters a = 55.63, b = 83.25, c = 120.75 A
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
most stable when stored at pH 8.0 with 2 and 3 M KCl
726769
8 - 9.8
-
-
287280
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
no loss of activity after 15 min, complete loss of activity at 60C
70
-
1.5 h, no loss of activity; 2 h, 10% loss of activity
100
-
half-life 1 h; half-life: 1 h
102
-
Tm-value in presence of 1 M NaCl
104
-
Tm-value in absence of NaCl
additional information
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thermal denaturation is fully irreversible and follows first-order kinetics
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
most stable when stored at pH 8.0 with 2 M and 3 M KCl
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetonitrile
dimethyl sulfoxide
Glycerol
Methanol
tetrahydrofuran
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 30 days, 80% residual activity
-20C, 30 days, enzyme retains 80% of its original activity
-20C, crude enzyme retains half of its original activity following incubation at -20C for 75 days and almost one third of its original activity following incubation for 42 days
-20C, retains approximately 80% of its original activity after 10 days
4C, inactive within 10 days
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, Sephadex G-100
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ammonium sulfate, DEAE-Sephadex A-50, hydroxyapatite, Sephadex G-200
-
ammonium sulfate, DEAE-Sephadex, Sephadex G-200, 55C for 6 min
-
chromatofocusing, reactive red
-
DEAE-Sephacel, ammonium sulfate, Sephacryl S300HR, Phenyl-Sepharose, Red A
-
DEAE-Sephacel, CM-cellulose, Sephacryl S300HR, Phenyl-Sepharose, Blue A/DEAE-Sephacel
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isolation of a gene, that is induced after culture filtrate treatment from Erwinia carotovora
purified in one step by immobilised Ni-affinity chromatography
purified in one step by immobilized metal-affinity chromatography
strain B592, DEAE-cellulose, hydroxyapatite, Sephacryl S-300, Matrex Gel Red A
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a hexahistidine-tagged recombinant version of the enzyme is heterologously overexpressed in Haloferax volcanii
expressed in Escherichia coli as a fusion protein with (His)6-tag and 13 amino acid linker at the N-terminal
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expressed in Escherichia coli; expression in Escherichia coli
-
expression in Escherichia coli
expression in insect SF9 cells
expresssion in Haloferax volcanii
homologously expressed
overexpression in Escherichia coli as a His-tagged protein