Information on EC 1.1.1.91 - aryl-alcohol dehydrogenase (NADP+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.91
-
RECOMMENDED NAME
GeneOntology No.
aryl-alcohol dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an aromatic alcohol + NADP+ = an aromatic aldehyde + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
aryl-alcohol:NADP+ oxidoreductase
Also acts on some aliphatic aldehydes, but cinnamaldehyde was the best substrate found.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-27-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
wheatgrass
-
-
Manually annotated by BRENDA team
alfalfa
-
-
Manually annotated by BRENDA team
American Type Culture Collection No.607
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
JCM3016
-
-
Manually annotated by BRENDA team
JCM3016
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
rye
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
tomato
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Triticale turgidocereale
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-fluorobenzaldehyde + NADPH
2-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 27% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
2-methoxybenzaldehyde + NADPH
2-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 58% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
2-nitrobenzaldehyde + NADPH
2-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 57% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3,4,5-trimethoxybenzaldehyde + NADPH
3,4,5-trimethoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxybenzaldehyde + NADPH
3,4-dihydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
3,4-dimethoxybenzaldehyde + NADH
3,4-dimethoxybenzyl alcohol + NAD+
show the reaction diagram
-
-
-
-
r
3,4-dimethoxybenzaldehyde + NADPH
3,4-dimethoxybenzyl alcohol + NADP+
show the reaction diagram
3,5-dimethoxybenzaldehyde + NADPH
3,5-dimethoxybenzyl alcohol + NADP+
show the reaction diagram
3-chlorobenzaldehyde + NADPH
3-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 73% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-fluorobenzaldehyde + NADPH
3-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 69% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-hydroxy-4-methoxy-benzaldehyde + NADPH
3-hydroxy-4-methoxy-benzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 17% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-hydroxy-4-methoxybenzaldehyde + NADPH + H+
3-hydroxy-4-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
3-methoxybenzaldehyde + NADPH
3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 117% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-nitrobenzaldehyde + NADPH
3-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 25% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-benzyloxy-3-methoxybenzaldehyde + NADPH + H+
4-benzyloxy-3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-chlorobenzaldehyde + NADPH
4-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 61% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-fluorobenzaldehyde + NADPH
4-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 29% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-hydroxy-3-methoxy-benzaldehyde + NADPH
4-hydroxy-3-methoxy-benzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 19% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-hydroxy-3-methoxybenzaldehyde + NADPH + H+
4-hydroxy-3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-hydroxybenzaldehyde + NADPH
4-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 34% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-hydroxybenzaldehyde + NADPH + H+
4-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-methoxybenzaldehyde + NADPH
4-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 110% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-methoxybenzyl alcohol + NADP+
4-methoxybenzaldehyde + NADPH
show the reaction diagram
4-nitrobenzaldehyde + NADPH
4-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 31% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
5-(hydroxymethyl)-2-furaldehyde + NADPH + H+
(furan-2,5-diyl)dimethanol + NADP+
show the reaction diagram
-
relative activity: 36% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
show the reaction diagram
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
show the reaction diagram
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
cinnamic alcohol + NADP+
cinnamic aldehyde + NADPH
show the reaction diagram
coniferyl alcohol + NADP+
coniferylaldehyde + NADPH + H+
show the reaction diagram
decanal + NADPH
decanol + NADP+
show the reaction diagram
-
relative activity: 2% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
heptaldehyde + NADPH
?
show the reaction diagram
-
relative activity: 26% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
hexanal + NADPH
hexanol + NADP+
show the reaction diagram
-
relative activity: 47% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
m-bromobenzaldehyde + NADPH + H+
m-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-chlorobenzaldehyde + NADPH + H+
m-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-fluorobenzaldehyde + NADPH + H+
m-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-hydroxybenzaldehyde + NADPH + H+
m-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-nitrobenzaldehyde + NADPH
m-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
n-butanol + NADP+
n-butanal + NADPH
show the reaction diagram
-
-
-
-
r
n-pentanol + NADP+
n-pentanal + NADPH
show the reaction diagram
-
-
-
-
r
n-propanol + NADP+
n-propanal + NADPH
show the reaction diagram
-
-
-
-
r
nonanal + NADPH
nonanol + NADP+
show the reaction diagram
-
relative activity: 3% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
o-bromobenzaldehyde + NADPH + H+
o-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
o-chlorobenzaldehyde + NADPH + H+
o-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
o-fluorobenzaldehyde + NADPH + H+
o-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
octanal + NADPH
octanol + NADP+
show the reaction diagram
-
relative activity: 37% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
p-aminobenzyl alcohol + NADP+
p-aminobenzaldehyd + NADPH
show the reaction diagram
-
-
-
-
r
p-bromobenzaldehyde + NADPH + H+
p-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-chlorobenzaldehyde + NADPH + H+
p-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-fluorobenzaldehyde + NADPH + H+
p-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-hydroxybenzaldehyde + NADPH + H+
p-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-methylbenzaldehyde + NADPH
p-methylbenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
pentanal + NADPH
pentanol + NADP+
show the reaction diagram
-
relative activity: 8% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
phenylacetaldehyde + NADPH
phenylethanol + NADP+
show the reaction diagram
-
relative activity: 109 (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
phenylacetaldehyde + NADPH + H+
phenylethanol + NADP+
show the reaction diagram
-
-
-
-
?
salicyl alcohol + NADP+
p-hydroxybenzaldehyde + NADPH
show the reaction diagram
-
i.e. p-hydroxybenzyl alcohol
-
-
r
trans-cinnamaldehyde + NADPH
?
show the reaction diagram
-
relative activity: 39% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
veratryl alcohol + NADP+
veratraldehyde + NADPH
show the reaction diagram
-
i.e. 3,4-dimethoxybenzyl alcohol
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
-
enzyme utizilizes either NAD+ or NADP+ in the oxidation
NADP+
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
3,4-dimethoxybenzaldehyde
-
-
3,4-dimethoxybenzyl alcohol
-
-
3,5-dimethoxybenzaldehyde
-
-
3-hydroxy-4-methoxy-benzaldehyde
-
-
4-hydroxy-3-methoxybenzaldehyde
-
-
5,5'-dithio-bis(2-nitrobenzoic acid)
-
-
5-(hydroxymethyl)-2-furaldehyde
-
-
AgNO3
diphenyl hydantoin
-
-
Iodosobenzoate
-
-
p-chloromercuribenzoate
trans-cinnamaldehyde
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012
3,4-dimethoxybenzaldehyde
-
pH 6.1, 30C
0.37
3,4-dimethoxybenzyl alcohol
-
pH 6.1, 30C
0.022
3,5-dimethoxybenzaldehyde
-
pH 6.1, 30C
1.4
3-hydroxy-4-methoxy-benzaldehyde
-
pH 6.1, 30C
0.55
3-phenylpropionaldehyde
-
-
1.4
4-Hydroxy-3-methoxy-benzaldehyde
-
pH 6.1, 30C
25
4-hydroxy-3-methoxy-benzyl alcohol
-
pH 6.1, 30C
0.09 - 0.5
4-methoxybenzaldehyde
1.7
4-methoxybenzyl alcohol
-
-
0.27
5-(hydroxymethyl)-2-furaldehyde
-
pH 6.1, 30C
1.7
acetaldehyde
-
-
0.34 - 1.7
benzaldehyde
0.057 - 4.35
benzyl alcohol
0.22
cinnamic aldehyde
-
-
0.55
coniferyl alcohol
-
-
0.14
coniferyl aldehyde
-
-
0.16
m-bromobenzaldehyde
-
-
0.1
m-chlorobenzaldehyde
-
-
0.42
m-fluorobenzaldehyde
-
-
1.1
m-hydroxybenzaldehyde
-
-
0.21
m-nitrobenzaldehyde
-
-
0.0026 - 0.22
NADH
0.024 - 0.18
NADP+
0.017 - 0.1
NADPH
0.25
o-bromobenzaldehyde
-
-
0.2
o-chlorobenzaldehyde
-
-
0.27
o-fluorobenzaldehyde
-
-
0.05
o-nitrobenzaldehyde
-
-
0.04
p-aminobenzyl alcohol
-
-
0.22
p-bromobenzaldehyde
-
-
0.34
p-chlorobenzaldehyde
-
-
0.26
p-fluorobenzaldehyde
-
-
0.09
p-Hydroxybenzaldehyde
-
-
0.3
p-methylbenzaldehyde
-
-
0.04
p-nitrobenzaldehyde
-
-
0.53
phenylacetaldehyde
-
pH 6.1, 30C
3.4
trans-cinnamaldehyde
-
pH 6.1, 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.833
3,4-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
2.55
3,4-dimethoxybenzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30C
9.83
3,5-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
3.83
3-hydroxy-4-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
3.33
4-Hydroxy-3-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
4.33
4-hydroxy-3-methoxy-benzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30C
8.16
4-methoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
2.93
5-(hydroxymethyl)-2-furaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
7.16
benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
2
NADH
Phanerochaete chrysosporium
-
pH 6.1, 30C
2.56
NADP+
Phanerochaete chrysosporium
-
pH 6.1, 30C
11.33
NADPH
Phanerochaete chrysosporium
-
pH 6.1, 30C
11.16
phenylacetaldehyde
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
733
3,4-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
2637
6.8
3,4-dimethoxybenzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30C
2814
450
3,5-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
7440
2.66
3-hydroxy-4-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
19772
2.33
4-Hydroxy-3-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
12368
0.166
4-hydroxy-3-methoxy-benzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30C
42614
90
4-methoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
1016
10
5-(hydroxymethyl)-2-furaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
19771
5
benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
146
10
NADH
Phanerochaete chrysosporium
-
pH 6.1, 30C
8
68
NADP+
Phanerochaete chrysosporium
-
pH 6.1, 30C
10
283
NADPH
Phanerochaete chrysosporium
-
pH 6.1, 30C
5
11.16
phenylacetaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
769
3.33
trans-cinnamaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30C
2225
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.4
3,4-dimethoxybenzaldehyde
-
pH 6.1, 30C
165
3,4-dimethoxybenzyl alcohol
-
pH 6.1, 30C
2.1
3,5-dimethoxybenzaldehyde
-
pH 6.1, 30C
2.3
3-hydroxy-4-methoxy-benzaldehyde
-
pH 6.1, 30C
5.1
4-hydroxy-3-methoxybenzaldehyde
-
pH 6.1, 30C
136
5-(hydroxymethyl)-2-furaldehyde
-
pH 6.1, 30C
81
benzaldehyde
-
pH 6.1, 30C
3.5
trans-cinnamaldehyde
-
pH 6.1, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4
-
reduction activity
8.8
-
alcohol oxidation
10.4
-
oxidation activity
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 7.2
-
in imidazole buffer
7 - 8
-
in phosphate buffer, activity of the enzyme is only one-half that shown in imidazole buffer
7.9 - 8.5
-
in Tris buffer, activity is about 40% as in imidazole buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 50
-
by raising the temperature of the incubation mixture a linear increase of the reaction up to 55C is noted, but the reaction over 45C stops after 60-75 s
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
-
gel filtration
280000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
-
4 * 47000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0C, potassium phosphate buffer pH 7.5, 1 mM mercaptoethanol, 2 weeks, no loss of activity
-
4C, 10 mM Tris/HCl pH 7.5, 6 days, 95% loss of activity
-
4C, 10 mM Tris/HCl pH 7.5, one month, 15% loss of activity
-
all buffers, standard buffer Tris-HCl , 5 mM mercaptoethanol and sucrose, 2.5 g/10 ml, in the final preparation, 2 weeks, little loss of activity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
-
Show AA Sequence (274 entries)
Please use the Sequence Search for a certain query.