Information on EC 2.3.1.22 - 2-acylglycerol O-acyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.3.1.22
-
RECOMMENDED NAME
GeneOntology No.
2-acylglycerol O-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acyl-CoA + 2-acylglycerol = CoA + diacylglycerol
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
monoacylglycerol metabolism (yeast)
-
-
Glycerolipid metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:2-acylglycerol O-acyltransferase
Various 2-acylglycerols can act as acceptor; palmitoyl-CoA and other long-chain acyl-CoAs can act as donors. The sn-1 position and the sn-3 position are both acylated, at about the same rate.
CAS REGISTRY NUMBER
COMMENTARY hide
9055-17-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ADP1, gene atfA
-
-
Manually annotated by BRENDA team
male
-
-
Manually annotated by BRENDA team
zebrafish
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
; higher mammals, humans, not in rodents
-
-
Manually annotated by BRENDA team
opossum
-
-
Manually annotated by BRENDA team
gene Mogat2
UniProt
Manually annotated by BRENDA team
no activity in Rodentia
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-acyl-sn-glycerol + acetyl-CoA
diacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
2-acyl-sn-glycerol + palmitoyl-CoA
diacylglycerol + CoA
show the reaction diagram
-
in vitro assay in lysate of transfected CHO cells or of microsome preparation of mouse liver, 2-monoacylglycerol is preferred over 1-monoacylglycerol, 25C, pH 6.4
-
-
?
2-monooleoylglycerol + palmitoyl-CoA
2-oleoyl-3-palmitoylglycerol + CoA
show the reaction diagram
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 2-acylglycerol
CoA + diacylglycerol
show the reaction diagram
acyl-CoA + sn-2-monoacylglycerol
CoA + diacylglycerol
show the reaction diagram
acyl-CoA + sn-2-monolinolenoylglycerol
CoA + 1-acyl-2-linolenoylglycerol
show the reaction diagram
-
in preference to sn-2-monooleoylglycerol
-
-
?
acyl-CoA + sn-2-monolinoleoylglycerol
CoA + 1-acyl-2-linoleoylglycerol
show the reaction diagram
-
in preference to sn-2-monooleoylglycerol
-
-
?
arachidonoyl-CoA + sn-2-monooleoylglycerol
CoA + 1-arachidonoyl-2-oleoylglycerol
show the reaction diagram
-
-
-
?
arachidoyl-CoA + sn-2-monooleoylglycerol
CoA + 1-arachidoyl-2-oleoylglycerol
show the reaction diagram
-
-
-
?
decanoyl-CoA + 2-oleoylglycerol
?
show the reaction diagram
-
-
-
?
fatty acyl-CoA + sn-2-monoacylglycerol
CoA + 1,2-diacylglycerol
show the reaction diagram
lauroyl-CoA + sn-2-monooleoylglycerol
CoA + 1-lauroyl-2-oleoylglycerol
show the reaction diagram
-
-
-
?
linoleoyl-CoA + sn-2-monooleoylglycerol
CoA + 1-linoleoyl-2-oleoylglycerol
show the reaction diagram
monoacylglycerol + acyl-CoA
diacylglycerol + CoA
show the reaction diagram
monoacylglycerol + fatty acyl-CoA
diacylglycerol + CoA
show the reaction diagram
n-octanoyl-CoA + sn-2-monooleoylglycerol
CoA + 1-octanoyl-2-oleoylglycerol
show the reaction diagram
-
-
-
?
N-[(7-nitro-2-1,3-benzoxadiazol-4-yl)-methyl]amino-palmitoyl-CoA + 2-oleoylglycerol
CoA + N-[(7-nitro-2-1,3-benzoxadiazol-4-yl)-methyl]amino-palmitoyl-diacylglycerol
show the reaction diagram
fluorescent-labeled substrate
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
?
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-monooleoylglycerol
CoA + dioleoylglycerol
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol
CoA + 1,2-dioleoyl-sn-glycerol
show the reaction diagram
oleoyl-CoA + 1-palmitoyl-sn-glycerol
CoA + 1-palmitoyl-3-oleoyl-sn-glycerol
show the reaction diagram
oleoyl-CoA + 2-oleoyl-sn-glycerol
?
show the reaction diagram
-
-
-
?
oleoyl-CoA + sn-1-monooleoylglycerol
CoA + sn-1,3-dioleoylglycerol
show the reaction diagram
-
-
?
oleoyl-CoA + sn-2-monooleoylglycerol
CoA + sn-1,2(2,3)-dioleoylglycerol
show the reaction diagram
oleoyl-CoA + sn-3-monostearoylglycerol
CoA + 1-oleoyl-3-stearoylglycerol
show the reaction diagram
-
-
?
palmitoyl-CoA + 1,2-dipalmitoylglycerol
CoA + tripalmitoylglycerol
show the reaction diagram
-
i.e. 1,2-dipalmitin
-
-
?
palmitoyl-CoA + 1,3-dipalmitoylglycerol
CoA + tripalmitoylglycerol
show the reaction diagram
-
i.e. 1,2-dipalmitin
-
-
?
palmitoyl-CoA + 1-palmitoyl-sn-glycerol
CoA + ?
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 2,3-dipalmitoylglycerol
CoA + tripalmitoylglycerol
show the reaction diagram
-
i.e. 1,2-dipalmitin
-
-
?
palmitoyl-CoA + 2-hexadecylglycerol
CoA + 2-hexadecyl-1-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 2-palmitoyl-sn-glycerol
CoA + 1,2-dipalmitoylglycerol
show the reaction diagram
palmitoyl-CoA + rac-1-monooleoylglycerol
CoA + ?
show the reaction diagram
-
intestine, at 78% the rate of sn-2-monooleoylglycerol acylation
-
-
?
palmitoyl-CoA + rac-1-monooleoylglycerol ether
CoA + ?
show the reaction diagram
-
intestine, at 45% the rate of sn-2-monooleoylglycerol acylation
-
-
?
palmitoyl-CoA + rac-1-monopalmitoylglycerol
CoA + ?
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + sn-1,2-dioleoylglycerol
CoA + 1,2-dioleoyl-3-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + sn-1-monooleoylglycerol
CoA + ?
show the reaction diagram
palmitoyl-CoA + sn-1-monooleylglycerol ether
CoA + ?
show the reaction diagram
-
at 5.7% of the activity with sn-2-monooleoylglycerol
-
-
?
palmitoyl-CoA + sn-2-monooleoylglycerol
CoA + 1(3)-palmitoyl-2-oleoylglycerol
show the reaction diagram
palmitoyl-CoA + sn-2-monooleylglycerol
CoA + 2-oleyl-1-palmitoylglycerol
show the reaction diagram
-
at 5.7% of the activity with sn-2-monooleoylglycerol
-
-
?
rac-1-linolenoylglycerol + oleoyl-CoA
1-linolenoyl-3-oleoylglycerol + CoA
show the reaction diagram
-
-
-
?
rac-1-linoleoylglycerol + oleoyl-CoA
1-linoleoyl-3-oleoylglycerol + CoA
show the reaction diagram
-
-
-
?
rac-1-monoacylglycerol + palmitoyl-CoA
diacylglycerol + CoA
show the reaction diagram
-
in vitro assay in lysate of transfected CHO cells, 25C, pH 6.4
-
-
?
rac-1-oleoylglycerol + oleoyl-CoA
1,3-dioleoylglycerol + CoA
show the reaction diagram
-
-
-
?
sn-2-oleoylglycerol + oleoyl-CoA
2,3-dioleoylglycerol + CoA
show the reaction diagram
-
-
-
?
stearoyl-CoA + 1-palmitoyl-sn-glycerol
CoA + ?
show the reaction diagram
-
-
-
-
?
stearoyl-CoA + sn-2-monooleoylglycerol
CoA + 1-stearoyl-2-oleolyglycerol
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-acyl-sn-glycerol + acetyl-CoA
diacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
2-monooleoylglycerol + palmitoyl-CoA
2-oleoyl-3-palmitoylglycerol + CoA
show the reaction diagram
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
-
-
-
-
?
acyl-CoA + 2-acylglycerol
CoA + diacylglycerol
show the reaction diagram
acyl-CoA + sn-2-monoacylglycerol
CoA + diacylglycerol
show the reaction diagram
monoacylglycerol + acyl-CoA
diacylglycerol + CoA
show the reaction diagram
-
-
-
-
?
monoacylglycerol + fatty acyl-CoA
diacylglycerol + CoA
show the reaction diagram
palmitoyl-CoA + sn-1,2-dioleoylglycerol
CoA + 1,2-dioleoyl-3-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
stimulates
Ca2+
-
stimulates
CuCl2
-
2.5 mM, stimulation
Na+
-
minimal stimulation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4,6-Trinitrobenzene sulfonic acid
2-monooleoylglycerol
-
high concentration
3,3-dimethyl-2,5-dioxo-N-phenyl-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
3-ethyl-2,5-dioxo-N-phenyl-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
3-ethyl-3-methyl-2,5-dioxo-N-phenyl-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
3-ethyl-3-methyl-2,5-dioxo-N-[4-(trifluoromethyl)phenyl]-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
3-ethyl-N,3-dimethyl-2,5-dioxo-N-phenyl-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
3-ethyl-N-(4-methoxyphenyl)-3-methyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
acetone
-
10%, liver
beta-octylglucoside
bovine serum albumin
-
Ca2+
-
14% inhibition at 2.5 mM
CaCl2
-
12.5 mM, 20-30% inhibition
CHAPS
deoxycholate
-
inhibition of liver enzyme
diethyldicarbonate
-
0.04 mM, 50% inhibition, 0.05 mM palmitate partly protects, completely reversible by 50 mM hydroxylamine, enzyme from intestine is less sensitive than enzyme from liver
HgCl2
KI
-
300 mM, 30% inhibition
Mg2+
-
66% inhibition at 8 mM
Mn2+
-
85.5% inhibition at 2.5 mM, inhibition of Mg2+ and bovine serum albumin is antagonistic
MnCl2
N-(2,4-difluorophenyl)-3,3-diethyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-(2,4-difluorophenyl)-3,3-diethyl-N-methyl-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-(2,4-difluorophenyl)-3,3-diethyl-N-methyl-2-oxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-(2,4-difluorophenyl)-3,3-diethyl-N-methyl-5-oxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-(2,4-difluorophenyl)-3-ethyl-3-methyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-(2,4-difluorophenyl)-3-ethyl-N,3,4-trimethyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-(2,4-difluorophenyl)-3-methyl-2,5-dioxo-3-propyl-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-(2-chlorophenyl)-3-ethyl-3-methyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-(3-chlorophenyl)-3-ethyl-3-methyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-(3-ethyl-3-methyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepin-7-yl)benzenesulfonamide
-
-
N-(4-chlorophenyl)-2,5-dioxo-1,2,4,5-tetrahydrospiro[1,4-benzodiazepine-3,1'-cyclobutane]-7-sulfonamide
-
-
N-(4-chlorophenyl)-2,5-dioxo-1,2,4,5-tetrahydrospiro[1,4-benzodiazepine-3,1'-cyclopentane]-7-sulfonamide
-
-
N-(4-chlorophenyl)-3-ethyl-3-methyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-(4-chlorophenyl)-3-ethyl-N,1,3-trimethyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-(4-chlorophenyl)-N,3,3-trimethyl-5-oxo-2,3,4,5-tetrahydro-1,4-benzoxazepine-7-sulfonamide
-
-
N-(4-chlorophenyl)-N-methyl-2,5-dioxo-1,2,4,5-tetrahydrospiro[1,4-benzodiazepine-3,1'-cyclobutane]-7-carboxamide
-
-
N-benzyl-3-ethyl-3-methyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-cyclohexyl-3-ethyl-3-methyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
N-[4-(difluoromethoxy)phenyl]-3-ethyl-3-methyl-2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine-7-sulfonamide
-
-
NEM
-
2 mM, 12% inhibition
oleic acid
-
-
palmitoyl-CoA
phytosphingosine
-
-
shRNA-adenovirus
-
short hairpin RNA adenovirus construct to knockdown mouse lysophosphatidylglycerol acyltransferase 1, injected via tail vein
-
sphinganine
-
-
sphingosine
-
IC50 is 9 mol%, inhibits activity in Triton X-100 mixed micelles and isolated hepatocytes
Stearylamine
-
-
taurocholate
Triton X-100
XP620
-
selective isozyme DGAT1 inhibitor, inhibits the diacylglycerol acyltransferase and the retinol acyltransferase activities at the retinol recognition site
additional information
-
identification, development, and optimisation of a novel series of MGAT2 inhibitors, pIC50 values, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
-
deoxycholate
-
stimulates enzyme from intestinal mucosa
oleic acid
-
stimulates below 0.015 mM
palmitic acid
-
0.02-0.05 mM, stimulates
phosphatidic acid
-
stimulates
phosphatidylcholine
-
stimulates
Phospholipid
-
phosphatidylcholine/phosphatidylserine at 0.025 mg/ml stimulates 80%
sn-1,2-dioleoylglycerol
sphingomyelin
sphingosine
-
0.0025-0.0075 mM, activates
Tween 20
-
maximal stimulation in presence of 1.4 mg/ml
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01481
1-oleoyl-sn-glycerol
pH and temperature not specified in the publication
0.00565
1-oleoylglycerol
-
-
0.01639
1-palmitoylglycerol
-
-
0.16
2-oleoyl-sn-glycerol
-
-
0.047
linoleoyl-CoA
-
-
0.00832 - 0.00935
oleoyl-CoA
0.0065 - 0.01754
palmitoyl-CoA
0.0071
sn-2-monolinolenoylglycerol
-
-
0.011
sn-2-monolinoleoylglycerol
-
-
0.021
sn-2-monooleoylglycerol
-
-
0.02566
stearoyl-CoA
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
inhibition kinetics depend highly on retinol concentration, overview, recombinant enzyme
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.118
-
purified recombinant enzyme, substrates palmitoyl-CoA and 1,3-dipalmitoylglycerol
0.211
-
purified recombinant enzyme, substrates palmitoyl-CoA and 1,2-dipalmitoylglycerol
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
7.8
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15
-
assay at
25
-
assay at
35
-
assay at
37
-
assay at
additional information
assay carried out at room temperature; assay carried out at room temperature; assay carried out at room temperature
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
predicted from sequence analysis
8.8
-
predicted from sequence analysis
8.9
-
predicted from sequence analysis
9
predicted from sequence analysis
9.3
predicted from sequence analysis
9.6
-
predicted from sequence analysis
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
high levels of activity in developing gut
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
low expression of MGAT2
Manually annotated by BRENDA team
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35320
-
predicted from sequence analysis, 4237 bp, 327 amino acids, gene location on chromosome 2
36000
-
SDS-PAGE
37710
predicted from sequence analysis, 10577 bp, 334 amino acids, gene location on chromosome 23
37800
predicted from sequence analysis, 5395 bp, 336 amino acids, gene location on chromosome 5
38000
-
recombinant enzyme, gel filtration, monomeric form
38010
predicted from sequence analysis, 23470 bp, 330 amino acids, gene location on chromosome 10
38390
-
predicted from sequence analysis, 30645 bp, 334 amino acids, gene location on chromosome 4
39130
-
predicted from sequence analysis, 32654 bp, 335 amino acids, gene location on chromosome 7
80000
-
the enzyme exists as both a homodimer and homotetramer, cross-linking
94100
-
recombinant enzyme, gel filtration, dimeric form
160000
-
the enzyme exists as both a homodimer and homotetramer, cross-linking
additional information
-
molecular weight for triacylglycerol synthetase complex of EC 2.3.1.20/EC 2.3.1.22 is 350000-370000 Da, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 53000, recombinant enzyme, SDS-PAGE
homodimer
-
2 * 38000, about, SDS-PAGE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48
-
12 min, microsomes from liver retain 90% of the initial activity
56
-
after microsomes from adult liver are incubated for 13 min, they retain 7% of the control activity. After 13.5 min of heating, neonatal liver microsomes retain 57% of the control activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70C, purified recombinant enzyme, no significant loss after several months
-
20C, the purified enzyme complex EC 2.3.1.20/2.3.1.22 loses 50% of its activity after 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
as part of triacylglycerol synthetase complex
-
oleosin-3 exists as part of a 14 S multiprotein complex, functioning as both an MGAT and a phospholipase A2
-
recombinant soluble enzyme from Escherichia coli strain BL21(DE3) by cation exchange, hydrophobic interaction, and anion exchange chromatography, 18.7fold
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Arabidopsis thaliana GPAT4 acyltransferase is expressed in Saccharomyces cerevisiae GPAT mutant
-
chromosomal location, expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells and in COS-7 cells
-
expression in COS-7 cells
-
expression of MGAT1 cDNA in Spodoptera frugiperda cells
expression of MGAT2 in AV-12, COS-7 and CACO-2 cell
-
gene atfA, expression of the soluble enzyme in Escherichia coli strain BL21(DE3)
-
gene MOGAT, co-expression of enzyme MGAT2 and acyl CoA:1,2-diacylglycerol acyltransferase, DGAT2, in HEK-293T and in COS-7 cells and co-localization in the endoplasmic reticulum and on lipid droplets
-
gene MOGAT, expression analysis, recombinant expression in enzyme-deficient mice
-
gene Mogat2, expression analysis, comparison of the relative expression levels of human MGAT2 in the transgenic mice to those of endogenous mouse MGAT2 in the wild-type mice
MGAT1 gene expression analysis by real-time PCR, the MGAT1 promoter is highly regulated by PPARgamma, phylogenetic analysis
-
MGAT3 in expressed in human embryonic kidney 293 (HEK293) cells
-
mouse MGAT2 acyltransferase is expressed in Nicotiana benthamiana
-
PCR-amplification, transfection and expression of CHO cells with FLAG-tagged enzyme cDNA plasmid
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
diabetes, obesity, and lactation induce liver monoacylglycerol acyltransferase activity in rodents that is correlated with increased fat absorption
-
hepatic LPGAT1 expression in diabetic (db/db) mice is 2fold higher than in control (db/m), consistent with 1.5fold higher hepatic monoacylglycerol acyltransferase activity
-
isozyme MGAT1 is highly induced up to 10fold by peroxisome proliferator-activated receptor gamma, PPARgamma, overexpression in human primary hepatocytes
-
marked weight loss following gastric bypass surgery is associated with a significant reduction in MOGAT2 and MOGAT3 expression; marked weight loss following gastric bypass surgery is associated with a significant reduction in MOGAT2 and MOGAT3 expression
the infection with a short hairpin RNA-adenovirus construct lowers the lysophosphatidylglycerol acyltransferase 1 expression in diabetic (db/db) mice compared to a control virus infection in a dose-dependent manner, causing a marked reduction in serum triacylglycerol and cholesterol levels, and a significant increase in hepatic cholesterol levels
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H125A/D130A
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no enzymatic activity
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
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[acyl CoA]monoacylglycerol acyltransferase 2 (MGAT2) is of interest as a target for therapeutic treatment of diabetes, obesity and other diseases which together constitute the metabolic syndrome
medicine
pharmacology
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[acyl CoA]monoacylglycerol acyltransferase 2 (MGAT2) is of interest as a target for therapeutic treatment of diabetes, obesity and other diseases which together constitute the metabolic syndrome