Information on EC 2.7.1.72 - streptomycin 6-kinase

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The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY hide
2.7.1.72
-
RECOMMENDED NAME
GeneOntology No.
streptomycin 6-kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + streptomycin = ADP + streptomycin 6-phosphate
show the reaction diagram
dATP can replace ATP, dihydrostreptomycin, streptidine and 2-deoxystreptidine can act as acceptors
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
streptomycin biosynthesis
-
-
Streptomycin biosynthesis
-
-
Biosynthesis of antibiotics
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-
SYSTEMATIC NAME
IUBMB Comments
ATP:streptomycin 6-phosphotransferase
dATP can replace ATP; and dihydrostreptomycin, streptidine and 2-deoxystreptidine can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-11-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC 14607
-
-
Manually annotated by BRENDA team
strain HUT 6037
-
-
Manually annotated by BRENDA team
strain HUT 6037
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-deoxydihydrostreptomycin + ATP
ADP + 3'-deoxydihydrostreptomycin 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + dihydrostreptomycin
ADP + dihydrostreptomycin 6-phosphate
show the reaction diagram
ATP + streptomycin
ADP + streptomycin 6-phosphate
show the reaction diagram
dATP + streptomycin
dADP + streptomycin 6-phosphate
show the reaction diagram
-
-
-
-
?
dihydrostreptomycin 3''-alpha-phosphate + ATP
ADP + dihydrostreptomycin 3''-alpha-phosphate 6-phosphate
show the reaction diagram
-
-
-
-
?
dihydrostreptomycin 3''-phosphate + ATP
ADP + dihydrostreptomycin 3'',6-bisphosphate
show the reaction diagram
-
-
-
-
?
streptidine + ATP
streptidine 6-phosphate + ADP
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + streptomycin
ADP + streptomycin 6-phosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
10 mM, rather less stimulatory than Mg2+
Cu2+
-
10 mM, rather less stimulatory than Mg2+
Fe2+
-
10 mM, slight stimulation
Zn2+
-
10 mM, slight stimulation
additional information
-
not affected by Ca2+, 0.2-100 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine
-
competitive
ADP
-
noncompetitive
AgNO3
-
strong inhibition
AMP
-
noncompetitive
dihydrostreptomycin
-
-
diphosphate
-
noncompetitive
EDTA
-
strong inhibition
streptomycin
-
-
additional information
-
no inhibition by sulfhydryl reagents, formamidine disulfide, p-chloromercuribenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11
dihydrostreptomycin
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-
28
streptidine
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-
3.5
streptomycin
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-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
adenosine
-
-
3
diphosphate
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11.35
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purified enzyme
additional information
-
assay methods
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
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assay at, method 1
additional information
-
pI: 6.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE