1.14.13.109: abieta-7,13-dien-18-ol hydroxylase

This is an abbreviated version, for detailed information about abieta-7,13-dien-18-ol hydroxylase, go to the full flat file.

Reaction

abieta-7,13-dien-18,18-diol
=
abieta-7,13-dien-18-al
+
H2O

Synonyms

abieta-7,13-dien-18-ol hydroxylase, abietadienol/abietadienal oxidase, CYP720B1, CyPA, CYPA1, CYPA2, CyPB, CYPC, CYPD, PtAO

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.109 abieta-7,13-dien-18-ol hydroxylase

Cloned

Cloned on EC 1.14.13.109 - abieta-7,13-dien-18-ol hydroxylase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CYP720B1, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, de novo diterpenoid formation in metabolically engineered yeast, overview; CYP720B1, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis; CYPA, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis; CYPB, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis; CYPC, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis
CYPA1, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis; CYPA2, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis
expression of GFP-fusion protein in transiently transformed tobacco cells. The first 48 amino of PtAO are fused to the N-terminus of green fluorescent protein (GFP) and expressed in tobacco under control of Cauliflower Mosaic Virus 35S promoter