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IUBMB Comments The enzyme, which operates in the opposite direction to that shown, forms a mixture of GDP-α-D -rhamnose and its C-4 epimer, GDP-6-deoxy-α-D -talose. cf . EC 1.1.1.281 , GDP-4-dehydro-6-deoxy-D -mannose reductase and EC 1.1.1.135 , GDP-6-deoxy-D -talose 4-dehydrogenase.
Synonyms
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GDP-4-keto-6-deoxy-D-mannose reductase
GDP-4-keto-D-rhamnose reductase
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guanosine diphosphate-4-keto-D-rhamnose reductase
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GDP-4-keto-6-deoxy-D-mannose reductase
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GDP-4-keto-6-deoxy-D-mannose reductase
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RMD
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tld gene product
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GDP-alpha-D-rhamnose + NAD(P)+ = GDP-4-dehydro-alpha-D-rhamnose + NAD(P)H + H+
In the reverse reaction, a mixture of GDP-D-rhamnose and its C-4 epimer is formed
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MetaCyc
GDP-6-deoxy-D-talose biosynthesis, GDP-D-rhamnose biosynthesis
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GDP-6-deoxy-alpha-D-mannose:NAD(P)+ 4-oxidoreductase
The enzyme, which operates in the opposite direction to that shown, forms a mixture of GDP-alpha-D-rhamnose and its C-4 epimer, GDP-6-deoxy-alpha-D-talose. cf. EC 1.1.1.281, GDP-4-dehydro-6-deoxy-D-mannose reductase and EC 1.1.1.135, GDP-6-deoxy-D-talose 4-dehydrogenase.
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GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-6-deoxy-D-mannose + NAD(P)+
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-D-talometylose + NAD(P)+
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Substrates: lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
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GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-D-talose + NADP+
GDP-4-dehydro-alpha-D-rhamnose + NAD(P)H + H+
GDP-alpha-D-rhamnose + NAD(P)+
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Substrates: -
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GDP-4-dehydro-alpha-D-rhamnose + NADH + H+
GDP-alpha-D-rhamnose + NAD+
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Substrates: -
r
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-6-deoxy-D-mannose + NAD(P)+
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Substrates: -
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GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-6-deoxy-D-mannose + NAD(P)+
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Substrates: lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
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GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-D-talose + NADP+
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Substrates: -
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GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-D-talose + NADP+
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Substrates: -
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GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-6-deoxy-D-mannose + NAD(P)+
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-D-talometylose + NAD(P)+
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Substrates: lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
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GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-6-deoxy-D-mannose + NAD(P)+
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Substrates: -
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GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-6-deoxy-D-mannose + NAD(P)+
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Substrates: lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
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NADH
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works equally well compared to NADPH
NADH
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works equally well compared to NADPH
NADPH
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GDP-alpha-D-mannose
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competitive inhibitor
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0.025 - 0.05
GDP-4-dehydro-6-deoxy-D-mannose
0.025
GDP-4-dehydro-6-deoxy-D-mannose
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with NADPH as reductant
0.05
GDP-4-dehydro-6-deoxy-D-mannose
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with NADH as reductant
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0.72
GDP-mannose
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competitive inhibition
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6 - 7
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with NADPH as reductant
8
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with NADH as reductant
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SUNYaB 75
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SUNYaB 75
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soil bacterium GS, ATCC 19241
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pathovar glycinea
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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malfunction
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effect of deficiency in GDP-D-rhamnose biosynthetic genes, including GDP-4-keto-6-deoxy-D-mannose reductase, on lipopolysaccharide structure and pathogenicity in soybean, Glycine max cv. Shirofumi. The mutant strain DELTArmd shows highly reduced antimicrobial susceptibility with chloramphenicol, ampicillinm or spectinomycin. The mutant DELTArmd strain also shows increased degrees of adhesion to the polystyrene surface of the micro-titrate plates. It has higher hydrophobicity of the cell surface than the wild-type strain, but has normal flagellin glycans
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B0RVL9_XANCB
Xanthomonas campestris pv. campestris (strain B100)
312
0
34119
TrEMBL
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A0A076NVK3_FLAPS
297
0
33441
TrEMBL
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Q93S89_XANCE
312
0
34119
TrEMBL
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M5CUK6_STEMA
308
0
33109
TrEMBL
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A6H035_FLAPJ
Flavobacterium psychrophilum (strain ATCC 49511 / DSM 21280 / CIP 103535 / JIP02/86)
297
0
33441
TrEMBL
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A0A8G2L8H3_FLAPS
297
0
33383
TrEMBL
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F8WJX0_BRUME
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
246
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26547
TrEMBL
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50000 - 70000
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gel filtration
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additional information
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construction of enzyme deletion mutant DELTArmd. The mutant strain shows highly reduced antimicrobial susceptibility with chloramphenicol, ampicillin or spectinomycin. Structural analysis of lipopolysaccharides in the mutant strain, NMR and mass spectrrometry analysis, overview
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56
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loss of activity in 5 min
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DTT reduces instability at room temperature from 51% to 35%
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389366
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-20°C, half-life: 5 months
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to homogeneity, fractionation-, chromatography-techniques
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expression in Escherichia coli
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Winkler, N.W.; Markovitz, A.
Guanosine diphosphate-4-keto-D-rhamnose reductase. A non-stereoselective enzyme
J. Biol. Chem.
246
5868-5876
1971
Pseudomonas sp.
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Barber, G.A.
The synthesis of guanosine 5-diphosphate D-rhamnose by enzymes of a higher plant
Biochim. Biophys. Acta
165
68-75
1968
Leucaena leucocephala
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Suzuki, N.; Nakano, Y.; Yoshida, Y.; Nezu, T.; Terada, Y.; Yamashita, Y.; Koga, T.
Guanosine diphosphate-4-keto-6-deoxy-D-mannose reductase in the pathway for the synthesis of GDP-6-deoxy-D-talose in Actinobacillus actinomycetemcomitans
Eur. J. Biochem.
269
5963-5971
2002
Aggregatibacter actinomycetemcomitans, Aggregatibacter actinomycetemcomitans SUNYaB 75
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Chiku, K.; Tsunemi, K.; Yamamoto, M.; Ohnishi-Kameyama, M.; Yoshida, M.; Ishii, T.; Taguchi, F.; Iwaki, M.; Ichinose, Y.; Ono, H.
Defects in D-rhamnosyl residue biosynthetic genes affect lipopolysaccharide structure, motility, and cell-surface hydrophobicity in Pseudomonas syringae pathovar glycinea race 4
Biosci. Biotechnol. Biochem.
77
505-510
2013
Pseudomonas syringae
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