Information on EC 1.14.13.189 - 5-methyl-1-naphthoate 3-hydroxylase

for references in articles please use BRENDA:EC1.14.13.189
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)


The expected taxonomic range for this enzyme is: Streptomyces sahachiroi

top print hide
EC NUMBER
COMMENTARY hide
1.14.13.189
-
top print hide
RECOMMENDED NAME
GeneOntology No.
5-methyl-1-naphthoate 3-hydroxylase
-
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-methyl-1-naphthoate + NADPH + H+ + O2 = 3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O
show the reaction diagram
-
-
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
5-methyl-1-naphthoate,NADPH:oxygen oxidoreductase (3-hydroxylating)
The enzyme from the bacterium Streptomyces sahachiroi is involved in the biosynthesis of 3-methoxy-5-methyl-1-naphthoate, a component of of the the antitumor antibiotic azinomycin B.
top
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the enzyme is involved in biosynthesis of 3-methoxy-5-methyl-1-naphthoate a component of of the the antitumor antibiotic azinomycin B
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-methyl-1-naphthoate + NADPH + H+ + O2
3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O
show the reaction diagram
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-methyl-1-naphthoate + NADPH + H+ + O2
3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O
show the reaction diagram
-
the enzyme is involved in biosynthesis of 3-methoxy-5-methyl-1-naphthoate a component of of the the antitumor antibiotic azinomycin B
-
-
?
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
a heme-thiolate protein (P450)
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.4
NADPH
-
pH 9.0, 25°C
top print hide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013
NADPH
-
pH 9.0, 25°C
top print hide
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0054
NADPH
-
pH 9.0, 25°C
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
-
pH 7.0: about 45% of maximal activity, pH 10.0: about 80% of maximal activity
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Streptomyces albus or in Escherichia coliBö21(DE3)
-
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.189)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)