Information on EC 1.3.8.11 - cyclohexane-1-carbonyl-CoA dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.8.11
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RECOMMENDED NAME
GeneOntology No.
cyclohexane-1-carbonyl-CoA dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cyclohexane-1-carbonyl-CoA + electron-transfer flavoprotein = cyclohex-1-ene-1-carbonyl-CoA + reduced electron-transfer flavoprotein
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
benzoate fermentation (to acetate and cyclohexane carboxylate)
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crotonate fermentation (to acetate and cyclohexane carboxylate)
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cyclohexane-1-carboxylate degradation (anaerobic)
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benzoyl-CoA degradation
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Benzoate degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
cyclohexane-1-carbonyl-CoA:electron transfer flavoprotein oxidoreductase
Contains FAD. The enzyme, characterized from the strict anaerobic bacterium Syntrophus aciditrophicus, is involved in production of cyclohexane-1-carboxylate, a byproduct produced by that organism during fermentation of benzoate and crotonate to acetate.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cyclohex-3-ene-1-carbonyl-CoA + ferricenium hexafluorophosphate
cyclohexadiene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
show the reaction diagram
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cyclohex-1-ene-1-carbonyl-CoA
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005 - 0.022
cyclohexane-1-carbonyl-CoA
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.049
cyclohex-1-ene-1-carbonyl-CoA
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at pH 7.0 and 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.1
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cell extract, at pH 7.0 and 30°C
52
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after 25fold purification, at pH 7.0 and 30°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
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4 * 42000, calculated from amino acid sequence
150000
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recombinant enzyme, gel filtration
155000
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native enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
octyl Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q ion exchange column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL 21 Star(DE3) OneShot cells
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