We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme is involved in the detoxification of the benzoxazinoids DIBOA (2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one) and DIMBOA (2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one) which are stored as the respective non-toxic glucosides in the vacuoles in some plants, most commonly from the family of Poaceae (grasses). Benzoxazinoids are known to exhibit antimicrobial, antifeedant, and antiinsecticidal effects and are involved in the interaction of plants with other plants, insects, or microorganisms.
The expected taxonomic range for this enzyme is: Zea mays
Synonyms
uridine diphosphoglucose-2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-glucosyltransferase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
uridine diphosphoglucose-2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-glucosyltransferase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
(2)
-
-
-
UDP-alpha-D-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
(1)
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hexosyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-glucose:2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase
The enzyme is involved in the detoxification of the benzoxazinoids DIBOA (2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one) and DIMBOA (2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one) which are stored as the respective non-toxic glucosides in the vacuoles in some plants, most commonly from the family of Poaceae (grasses). Benzoxazinoids are known to exhibit antimicrobial, antifeedant, and antiinsecticidal effects and are involved in the interaction of plants with other plants, insects, or microorganisms.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-glucose + 2,4-dihydroxy-1,4-benzoxazin-3-one
UDP + 2,4-dihydroxy-2H-1,4-benzoxazin-3-one 2-D-glucoside
-
peak 1 glucosyltransferase has 3.6% of activity compared to activity towards 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, peak 2 glucosyltransferase has 57% of activity compared to activity towards 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
-
-
?
UDP-glucose + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one
UDP + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
-
-
?
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
UDP-glucose + 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one
UDP + 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
low glucosylation efficiency
-
-
?
UDP-glucose + 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
UDP + 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
-
-
-
?
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
-
-
-
?
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
-
-
?
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
involved in detoxification of benzoxazinoids
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
-
-
-
?
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
involved in detoxification of benzoxazinoids
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ascorbate
-
at 5 mM, stimulation of 42%
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CaCl2
-
5 mM, stimulation
MgCl2
-
5 mM, stimulation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Cu2+
-
5 mM, 3% remaining activity
EDTA
-
16% decrease of activity
Fe2+
-
5 mM, 5% remaining activity
N-ethylmaleimide
-
at 1 mM, 96% decrease of activity, after addition of dithioerythritol, full activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-mercaptoethanol
-
5 mM, stimulation of 79%
ascorbate
-
at 5 mM, stimulation of 42%
dithioerythritol
-
5 mM, stimulation of 96%
glutathione
-
5 mM, stimulation of 92%
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.638
2,4-dihydroxy-1,4-benzoxazin-3-one
-
enzyme in peak 2
0.061 - 1.3
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one
0.071 - 0.217
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
0.081 - 0.096
UDP-glucose
0.061
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one
-
BX8-gene encoded protein
1.3
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one
-
BX9-gene encoded protein
0.071
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
-
BX9-gene encoded protein
0.081
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
-
BX8-gene encoded protein
0.174
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
-
enzyme in peak 2
0.217
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
-
enzyme in peak 1
0.081
UDP-glucose
-
BX8-gene encoded protein
0.096
UDP-glucose
-
BX9-gene encoded protein
0.28
UDPglucose
-
reaction with 2,4-dihydroxy-1,4-benzoxazin-3-one, enzyme in peak 2
0.286
UDPglucose
-
reaction with 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, enzyme in peak 1
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.218
-
enzyme in peak 1 and peak 2
1.4
-
2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, BX8-gene encoded protein
13
-
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX9-gene encoded protein
13.4
-
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX8-gene encoded protein
2.6
-
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX9-gene encoded protein
24
-
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX8-gene encoded protein
6
-
2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, BX9-gene encoded protein
0.5
-
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one, BX8-gene encoded protein
0.5
-
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one, BX9-gene encoded protein
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.5 - 10
-
10% of maximal activity at pH 6.5 and 30% of maximal activity at pH 10.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
25 - 55
-
88% of maximal activity at 55°C, 60% of maximal activity at 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
two different enzymes with different substrate specificities, genes Bx8 and Bx9
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
BX8_MAIZE
459
0
49470
Swiss-Prot
Mitochondrion (Reliability: 3 )
BX9_MAIZE
462
0
50017
Swiss-Prot
Mitochondrion (Reliability: 3 )
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
50000
-
gel filtration, both peaks
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
partial, activity elutes from Q-Sepharose column in two peaks, named peak 1 and peak 2, exhibiting different activities towards substrates
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli
-
transfected into Arabidopsis
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
von Rad, U.; Huttl, R.; Lottspeich, F.; Gierl, A.; Frey, M.
Two glucosyltransferases are involved in detoxification of benzoxazinoids in maize
Plant J.
28
633-642
2001
Zea mays
brenda
Bailey, B.A.; Larson, R.L.
Hydroxamic acid glucosyltransferases from maize seedlings
Plant Physiol.
90
1071-1076
1989
Zea mays
brenda
Select items on the left to see more content.
html completed