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alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
lacto-N-biose + phosphate
-
-
-
-
?
alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
beta D-galactosido-(1,3)-N-acetylgalactosamine + H2O
alpha-D-galactose-1-phosphate + GalNAc
-
-
-
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactopyranose 1-phosphate + N-acetyl-D-galactosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-glucosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosoamine + phosphate
alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine
galacto-N-biose + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
lacto-N-biose I + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
additional information
?
-
alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine + phosphate
-
-
-
?
alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine + phosphate
-
-
-
?
alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
-
-
-
-
?
alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
-
-
-
-
?
alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
-
-
-
r
alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
-
-
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactopyranose 1-phosphate + N-acetyl-D-galactosamine
-
-
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactopyranose 1-phosphate + N-acetyl-D-galactosamine
-
-
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
specific activity on N-acetyl-D-galactosamine is approximately 60times higher than that on N-acetyl-D-glucosamine
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
specific activity on N-acetyl-D-galactosamine is approximately 60times higher than that on N-acetyl-D-glucosamine
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine is galacto-N-biose
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine is galacto-N-biose
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
the gene operon for LNBP lnpABCD encodes a lacto-N-biose I and beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine metabolic pathway with lacto-N-biose phosphorylase, N-acetylhexosamine 1-kinase, UDP-glucose hexose 1-phosphate uridylyltransferase, and UDP-glucose 4-epimerase, this pathway is involved in the intestinal colonization of bifidobacteria and utilizes lacto-N-biose I from human milk oligosaccharides or beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine from mucin sugars
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
the gene operon for LNBP lnpABCD encodes a lacto-N-biose I and beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine metabolic pathway with lacto-N-biose phosphorylase, N-acetylhexosamine 1-kinase, UDP-glucose hexose 1-phosphate uridylyltransferase, and UDP-glucose 4-epimerase, this pathway is involved in the intestinal colonization of bifidobacteria and utilizes lacto-N-biose I from human milk oligosaccharides or beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine from mucin sugars
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
no activity with D-glucose, D-glucosamine, D-fructose, 2-deoxy-D-glucose, D-mannose, maltose, and sucrose as substrates
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
-
-
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
-
-
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
-
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
-
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine is lacto-N-biose I, best substrate being 7times more effective than beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine is lacto-N-biose I, best substrate being 7times more effective than beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-glucosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-glucosamine
-
-
-
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosoamine + phosphate
alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosoamine + phosphate
alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine
-
hydrolysis involves a Walden inversion
-
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosoamine + phosphate
alpha-D-galactopyranose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
r
galacto-N-biose + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
-
-
?
galacto-N-biose + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
-
-
r
lacto-N-biose I + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
?
lacto-N-biose I + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
r
lacto-N-biose I + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
-
-
?
additional information
?
-
substrate specificity, overview. Structurally determined substrate preference for galacto-N-biose and lacto-N-biose I
-
-
?
additional information
?
-
the enzyme reversibly phosphorolyzes galacto-N-biose (Galbeta1, 3GalNAc, GNB) and/or lacto-N-biose I (Galbeta1,3GlcNAc,LNB) to form alpha-galactose 1-phosphate (Gal1P) and the corresponding N-acetylhexosamine
-
-
?
additional information
?
-
the enzyme reversibly phosphorolyzes galacto-N-biose (Galbeta1, 3GalNAc, GNB) and/or lacto-N-biose I (Galbeta1,3GlcNAc,LNB) to form alpha-galactose 1-phosphate (Gal1P) and the corresponding N-acetylhexosamine
-
-
?
additional information
?
-
GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate
-
-
?
additional information
?
-
GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate
-
-
?
additional information
?
-
-
GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate
-
-
?
additional information
?
-
GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate
-
-
?
additional information
?
-
GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate
-
-
?
additional information
?
-
Cphy0577 protein does not show activity on either L-Rha, D-Glc, or D-Gal
-
-
?
additional information
?
-
Cphy0577 protein does not show activity on either L-Rha, D-Glc, or D-Gal
-
-
?
additional information
?
-
-
Cphy0577 protein does not show activity on either L-Rha, D-Glc, or D-Gal
-
-
?
additional information
?
-
Cphy3030 protein does not show activity on either L-Rha, D-Glc, or D-Gal
-
-
?
additional information
?
-
Cphy3030 protein does not show activity on either L-Rha, D-Glc, or D-Gal
-
-
?
additional information
?
-
-
Cphy3030 protein does not show activity on either L-Rha, D-Glc, or D-Gal
-
-
?
additional information
?
-
no activity with D-glucose, D-galactose, D-glucosamine, D-fructose, 2-deoxy-D-glucose, D-mannose, maltose, and sucrose
-
-
?
additional information
?
-
no activity with D-glucose, D-galactose, D-glucosamine, D-fructose, 2-deoxy-D-glucose, D-mannose, maltose, and sucrose
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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1.6 - 24
alpha-D-galactose 1-phosphate
10 - 140
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine
1.7 - 5.6
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine
2.1 - 1300
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
6.7 - 120
galacto-N-biose
3.3 - 11
N-acetyl-D-galactosamine
1.9 - 130
N-acetyl-D-glucosamine
1.6
alpha-D-galactose 1-phosphate
-
-
1.72
alpha-D-galactose 1-phosphate
wild type enzyme, using N-acetyl-D-glucosamine as cosubstrate
17
alpha-D-galactose 1-phosphate
mutant enzyme Y362F, using N-acetyl-D-glucosamine as cosubstrate
19
alpha-D-galactose 1-phosphate
mutant enzyme F364N, using N-acetyl-D-glucosamine as cosubstrate
24
alpha-D-galactose 1-phosphate
mutant enzyme N166A, using N-acetyl-D-glucosamine as cosubstrate
10
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine
in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C
140
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
1.7
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine
-
sequential bi-bi mechanism
5.6
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine
-
-
2.1
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
-
-
3.6
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C
21
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
-
-
1300
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
6.7
galacto-N-biose
pH 7.5, 30°C, mutant V162T
7.7
galacto-N-biose
pH 7.5, 30°C, wild-type enzyme
20
galacto-N-biose
pH 7.5, 30°C, mutant S336A
27
galacto-N-biose
pH 7.5, 30°C, mutant P161S
120
galacto-N-biose
pH 7.5, 30°C, mutant P161S/S336A
2.8
lacto-N-biose I
-
2.8
lacto-N-biose I
pH 7.5, 30°C, wild-type enzyme
8.4
lacto-N-biose I
pH 7.5, 30°C, mutant P161S
13
lacto-N-biose I
pH 7.5, 30°C, mutant S336A
22
lacto-N-biose I
pH 7.5, 30°C, mutant V162T
46
lacto-N-biose I
pH 7.5, 30°C, mutant P161S/S336A
3.3
N-acetyl-D-galactosamine
-
11
N-acetyl-D-galactosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
1.9
N-acetyl-D-glucosamine
-
3.4
N-acetyl-D-glucosamine
-
130
N-acetyl-D-glucosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
0.064
phosphate
with beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine as cosubstrate, in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C
0.43
phosphate
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine as substrate
0.52
phosphate
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine as substrate
0.58
phosphate
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine as substrate
0.66
phosphate
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine as substrate
1.3
phosphate
with beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine as cosubstrate, in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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D108N
-
site-directed mutagenesis
D160N
-
site-directed mutagenesis
D182N
-
site-directed mutagenesis
D232N
-
site-directed mutagenesis
D260N
-
site-directed mutagenesis
D280N
-
site-directed mutagenesis
D29N
-
site-directed mutagenesis
D313N
-
site-directed mutagenesis
D331N
-
site-directed mutagenesis
D344N
-
site-directed mutagenesis
D348N
-
site-directed mutagenesis
D35N
-
site-directed mutagenesis
D373N
-
site-directed mutagenesis
D490N
-
site-directed mutagenesis
D491N
-
site-directed mutagenesis
D561N
-
site-directed mutagenesis
E249Q
-
site-directed mutagenesis
E259Q
-
site-directed mutagenesis
E319Q
-
site-directed mutagenesis
E356Q
-
site-directed mutagenesis
E377Q
-
site-directed mutagenesis
E475Q
-
site-directed mutagenesis
E568Q
-
site-directed mutagenesis
E691Q
-
site-directed mutagenesis
D313N
mutant with undetectable activity
F364N
mutant shows severely impaired activity
N166A
mutant shows severely impaired activity
P161S
site-directed mutagenesis, the mutation leads to an increase in the selectivity on lacto-N-biose I
P161S/S336A
site-directed mutagenesis
R210E
the mutant shows no detectable activity
R32E
the mutant shows no detectable activity
R358E
the mutant shows no detectable activity
S336A
site-directed mutagenesis, the mutation leads to an increase in the selectivity on lacto-N-biose I
V162T
site-directed mutagenesis, the mutation leads to an increase in the selectivity on galacto-N-biose
Y362F
the mutation results in about 1000fold reduction of the catalytic efficiency
Y362N
the mutation results in the complete loss of the activity
C236E
random mutagenesis, the mutant shows decreased thermostability and reduced activity compared to the wild-type
C236F
random mutagenesis, the mutant shows highly increased thermostability and inacreased activity compared to the wild-type
C236H
random mutagenesis, the mutant shows slightly increased thermostability compared to the wild-type
C236P
random mutagenesis, the mutant shows highly increased thermostability and reduced activity compared to the wild-type
C236W
random mutagenesis, the mutant shows slightly increased thermostability compared to the wild-type
C236Y
random mutagenesis, the mutant shows highly increased thermostability and increased activity compared to the wild-type. In the mutant, the hydroxyl group of Tyr236 forms a hydrogen bond with the carboxyl group of E319. Mutant C236Y shows a 1.6fold higher specific activity than the wild-type
C236Y/D576V
random mutagenesis, the mutant shows highly increased thermostability compared to the wild-type
D576A
random mutagenesis, the mutant shows highly increased thermostability compared to the wild-type
D576F
random mutagenesis, the mutant shows highly increased thermostability and reduced activity compared to the wild-type
D576G
random mutagenesis, the mutant shows highly increased thermostability and increased activity compared to the wild-type
D576I
random mutagenesis, the mutant shows highly increased thermostability compared to the wild-type
D576L
random mutagenesis, the mutant shows highly increased thermostability compared to the wild-type
D576M
random mutagenesis, the mutant shows highly increased thermostability compared to the wild-type
D576P
random mutagenesis, the mutant shows decreased thermostability compared to the wild-type
D576V
random mutagenesis, the mutant shows highly increased thermostability compared to the wild-type
D576W
random mutagenesis, the mutant shows highly increased thermostability and reduced activity compared to the wild-type
G437S
random mutagenesis, the mutant shows slightly increased thermostability and slightly reducd activity compared to the wild-type
N506S
random mutagenesis, the mutant shows slightly increased thermostability and reduced activity compared to the wild-type
R290H
random mutagenesis, the mutant shows slightly increased thermostability and slightly reduced activity compared to the wild-type
R290H/G437S/N506S
random mutagenesis, the mutant shows highly increased thermostability and reduced activity compared to the wild-type
C236Y
-
random mutagenesis, the mutant shows highly increased thermostability and increased activity compared to the wild-type. In the mutant, the hydroxyl group of Tyr236 forms a hydrogen bond with the carboxyl group of E319. Mutant C236Y shows a 1.6fold higher specific activity than the wild-type
-
D576A
-
random mutagenesis, the mutant shows highly increased thermostability compared to the wild-type
-
D576V
-
random mutagenesis, the mutant shows highly increased thermostability compared to the wild-type
-
R290H
-
random mutagenesis, the mutant shows slightly increased thermostability and slightly reduced activity compared to the wild-type
-
additional information
enzyme engineering for improved thermostability, random mutagenesis GLNBP library construction using error-prone polymerase chain reaction and mutant screening, overview
additional information
-
enzyme engineering for improved thermostability, random mutagenesis GLNBP library construction using error-prone polymerase chain reaction and mutant screening, overview
-
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Derensy-Dron, D.; Krzewinski, F.; Brassart, C.; Bouquelet, S.
beta-1,3-Galactosyl-N-acetylhexosamine phosphorylase from Bifidobacterium bifidum DSM 20082: characterization, partial purification and relation to mucin degradation
Biotechnol. Appl. Biochem.
29
3-10
1999
Bifidobacterium bifidum
brenda
Kitaoka, M.; Tian, J.; Nishimoto, M.
Novel putative galactose operon involving lacto-N-biose phosphorylase in Bifidobacterium longum
Appl. Environ. Microbiol.
71
3158-3162
2005
Bifidobacterium longum (E8MF13), Bifidobacterium longum, Bifidobacterium bifidum (Q5NU16), Bifidobacterium bifidum
brenda
Nishimoto, M.; Kitaoka, M.
Identification of N-acetylhexosamine 1-kinase in the complete lacto-N-biose I/galacto-N-biose metabolic pathway in Bifidobacterium longum
Appl. Environ. Microbiol.
73
6444-6449
2007
Bifidobacterium longum subsp. Longum, Bifidobacterium longum subsp. Longum JCM 1217
brenda
Nakajima, M.; Nihira, T.; Nishimoto, M.; Kitaoka, M.
Identification of galacto-N-biose phosphorylase from Clostridium perfringens ATCC13124
Appl. Microbiol. Biotechnol.
78
465-471
2008
Bifidobacterium longum subsp. Longum, Clostridium perfringens ATCC 13124, Bifidobacterium longum subsp. Longum JCM 1217
brenda
Nishimoto, M.; Kitaoka, M.
Identification of the putative proton donor residue of lacto-N-biose phosphorylase (EC 2.4.1.211)
Biosci. Biotechnol. Biochem.
71
1587-1591
2007
Bifidobacterium bifidum JCM 1254
brenda
Nishimoto, M.; Kitaoka, M.
Practical preparation of lacto-N-biose I, a candidate for the bifidus factor in human milk
Biosci. Biotechnol. Biochem.
71
2101-2104
2007
Bifidobacterium bifidum JCM 1254
brenda
Nakajima, M.; Nishimoto, M.; Kitaoka, M.
Characterization of three beta-galactoside phosphorylases from clostridium phytofermentans: discovery of D-galactosyl-beta1,4-L-rhamnose phosphorylase
J. Biol. Chem.
284
19220-19227
2009
Lachnoclostridium phytofermentans (A9KIW5), Lachnoclostridium phytofermentans (A9KQ75), Lachnoclostridium phytofermentans
brenda
Nakajima, M.; Kitaoka, M.
Identification of lacto-N-biose I phosphorylase from Vibrio vulnificus CMCP6
Appl. Environ. Microbiol.
74
6333-6337
2008
Vibrio vulnificus (A0A3Q0KZS1), Vibrio vulnificus CMCP6LNBP (A0A3Q0KZS1)
brenda
Xiao, J.Z.; Takahashi, S.; Nishimoto, M.; Odamaki, T.; Yaeshima, T.; Iwatsuki, K.; Kitaoka, M.
Distribution of in vitro fermentation ability of lacto-N-biose I, a major building block of human milk oligosaccharides, in bifidobacterial strains
Appl. Environ. Microbiol.
76
54-59
2010
Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, no activity in Bifidobacterium catenulatum, no activity in Bifidobacterium animalis subsp. lactis, no activity in Bifidobacterium thermophilum, no activity in Bifidobacterium pseudocatenulatum, no activity in Bifidobacterium adolescentis, no activity in Bifidobacterium dentium, no activity in Bifidobacterium angulatum
brenda
Nakajima, M.; Nishimoto, M.; Kitaoka, M.
Characterization of beta-1,3-galactosyl-N-acetylhexosamine phosphorylase from Propionibacterium acnes
Appl. Microbiol. Biotechnol.
83
109-115
2009
Cutibacterium acnes (B7XEJ0), Cutibacterium acnes (B7XEJ1), Cutibacterium acnes, Cutibacterium acnes JCM 6425 (B7XEJ1), Cutibacterium acnes JCM 6473 (B7XEJ0)
brenda
Nishimoto, M.; Kitaoka, M.
One-pot enzymatic production of beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-D-galactose (galacto-N-biose) from sucrose and 2-acetamido-2-deoxy-D-galactose (N-acetylgalactosamine)
Carbohydr. Res.
344
2573-2576
2009
Bifidobacterium longum, Bifidobacterium longum JCM 1217
brenda
Suzuki, R.; Wada, J.; Katayama, T.; Fushinobu, S.; Wakagi, T.; Shoun, H.; Sugimoto, H.; Tanaka, A.; Kumagai, H.; Ashida, H.; Kitaoka, M.; Yamamoto, K.
Structural and thermodynamic analyses of solute-binding protein from Bifidobacterium longum specific for core 1 disaccharide and lacto-N-biose I
J. Biol. Chem.
283
13165-13173
2008
Bifidobacterium longum (A8W790), Bifidobacterium longum, Bifidobacterium longum JCM 1217 (A8W790)
brenda
Hidaka, M.; Nishimoto, M.; Kitaoka, M.; Wakagi, T.; Shoun, H.; Fushinobu, S.
The crystal structure of galacto-N-biose/lacto-N-biose I phosphorylase: a large deformation of a TIM barrel scaffold
J. Biol. Chem.
284
7273-7283
2009
Bifidobacterium longum (E8MF13), Bifidobacterium longum
brenda
Nishimoto, M.; Hidaka, M.; Nakajima, M.; Fushinobu, S.; Kitaoka, M.
Identification of amino acid residues that determine the substrate preference of 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase
J. Mol. Catal. B
74
97-102
2012
Bifidobacterium longum (E8MF13)
-
brenda
Koyama, Y.; Hidaka, M.; Nishimoto, M.; Kitaoka, M.
Directed evolution to enhance thermostability of galacto-N-biose/lacto-N-biose I phosphorylase
Protein Eng. Des. Sel.
26
755-761
2013
Bifidobacterium longum subsp. Longum (E8MF13), Bifidobacterium longum subsp. Longum JCM 1217 (E8MF13)
brenda
Nishimoto, M.
Large scale production of lacto-N-biose I, a building block of type I human milk oligosaccharides, using sugar phosphorylases
Biosci. Biotechnol. Biochem.
84
17-24
2020
Bifidobacterium longum subsp. Longum (E8MF13), Bifidobacterium longum subsp. Longum ATCC 15707 (E8MF13)
brenda