Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-glucuronate
4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-glucuronate
4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
additional information
?
-
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
-
-
-
?
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
-
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
-
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
-
-
-
?
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
-
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
-
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bibi mechanism
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bibi mechanism
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bibi mechanism
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
-
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
-
-
-
r
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1 phosphate + D-glucuronate
4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
-
-
-
r
alpha-D-glucose 1 phosphate + D-glucuronate
4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucuronate
4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
in the synthetic direction the enzyme produces 4-O-beta-D-glucopyranosyl-D-glucuronate from alpha-D-glucose 1-phosphate and D-glucuronate with low activity
-
-
?
alpha-D-glucose 1-phosphate + D-glucuronate
4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
in the synthetic direction the enzyme produces 4-O-beta-D-glucopyranosyl-D-glucuronate from alpha-D-glucose 1-phosphate and D-glucuronate with low activity
-
-
?
alpha-D-glucose 1-phosphate + D-glucuronate
4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucuronate
4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
-
-
-
r
additional information
?
-
no substrate: cellobiose
-
-
?
additional information
?
-
-
no substrate: cellobiose
-
-
?
additional information
?
-
no substrate: cellobiose
-
-
?
additional information
?
-
sequential bi-bi mechanism
-
-
?
additional information
?
-
-
sequential bi-bi mechanism
-
-
?
additional information
?
-
sequential bi-bi mechanism
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
-
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
-
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
-
-
-
r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
-
-
-
r
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
-
-
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
synthesis
an engineered strain of Neurospora crassa (F5) with six of seven beta-glucosidase (bgl) genes knocked out produces cellobiose and cellobionate directly from cellulose without the addition of exogenous cellulases. Further modification by knock-out of catabolite repression genes, cre-1 and ace-1, leads to improved cellobiose dehydrogenase and exoglucanase expression but not to an improvement in cellobiose or cellobionate production. Deletion of the cellobionate phosphorylase gene NdvB from the genome of F5 lacking ace-1 and cre-1 to prevent the consumption of cellobiose and cellobionate results in a slightly reduced hydrolysis rate and in convertsion of 75% of the cellulose consumed to the desired products, cellobiose and cellobionate, compared to 18% converted by the strain F5 lackong only ace-1 cre-1
synthesis
-
engineering of recombinant Neurospora crassa strains to produce cellobionate. Recombinant strains heterologously express laccase genes from different sources in Neurospora crassa which has six out of seven beta-glucosidase, two transcription factors, and the cellobionate phosphorylase (ndvB) genes deleted. The engineered strain produces 47.4 mM cellobionate from cellulose without any enzyme addition
synthesis
-
engineering of recombinant Neurospora crassa strains to produce cellobionate. Recombinant strains heterologously express laccase genes from different sources in Neurospora crassa which has six out of seven beta-glucosidase, two transcription factors, and the cellobionate phosphorylase (ndvB) genes deleted. The engineered strain produces 47.4 mM cellobionate from cellulose without any enzyme addition
-
synthesis
-
an engineered strain of Neurospora crassa (F5) with six of seven beta-glucosidase (bgl) genes knocked out produces cellobiose and cellobionate directly from cellulose without the addition of exogenous cellulases. Further modification by knock-out of catabolite repression genes, cre-1 and ace-1, leads to improved cellobiose dehydrogenase and exoglucanase expression but not to an improvement in cellobiose or cellobionate production. Deletion of the cellobionate phosphorylase gene NdvB from the genome of F5 lacking ace-1 and cre-1 to prevent the consumption of cellobiose and cellobionate results in a slightly reduced hydrolysis rate and in convertsion of 75% of the cellulose consumed to the desired products, cellobiose and cellobionate, compared to 18% converted by the strain F5 lackong only ace-1 cre-1
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Nihira, T.; Saito, Y.; Nishimoto, M.; Kitaoka, M.; Igarashi, K.; Ohtsubo, K.; Nakai, H.
Discovery of cellobionic acid phosphorylase in cellulolytic bacteria and fungi
FEBS Lett.
587
3556-3561
2013
Neurospora crassa (Q7S0S2), Neurospora crassa, Xanthomonas campestris (Q8P3J4), Xanthomonas campestris, Xanthomonas campestris pv. campestris (Q8P3J4), Neurospora crassa DSM 1257 (Q7S0S2), Xanthomonas campestris pv. campestris ATCC 33913 (Q8P3J4)
brenda
Hildebrand, A.; Szewczyk, E.1, Lin, H.; Kasuga, T.; Fan, Z.
Engineering Neurospora crassa for improved cellobiose and cellobionate production
Appl. Environ. Microbiol.
81
597-603
2015
Neurospora crassa (Q7S0S2), Neurospora crassa DSM 1257 (Q7S0S2)
brenda
Li, X.; Chomvong, K.; Yu, V.Y.; Liang, J.M.; Lin, Y.; Cate, J.H.
Cellobionic acid utilization: from Neurospora crassa to Saccharomyces cerevisiae
Biotechnol. Biofuels
8
120
2015
Neurospora crassa (Q7S0S2), Neurospora crassa, Neurospora crassa DSM 1257 (Q7S0S2)
brenda
Nam, Y.W.; Nihira, T.; Arakawa, T.; Saito, Y.; Kitaoka, M.; Nakai, H.; Fushinobu, S.
Crystal structure and substrate recognition of cellobionic ccid phosphorylase, which plays a key role in oxidative cellulose degradation by microbes
J. Biol. Chem.
290
18281-18292
2015
Saccharophagus degradans (Q21MB1), Saccharophagus degradans, Saccharophagus degradans DSM 17024 (Q21MB1)
brenda
Lin, H.; Hildebrand, A.; Kasuga, T.; Fan, Z.
Engineering Neurospora crassa for cellobionate production directly from cellulose without any enzyme addition
Enzyme Microb. Technol.
99
25-31
2017
Neurospora crassa, Neurospora crassa FGSC 2489
brenda