the enzyme is absolutely specific for D-glucuronate and ATP, no activity with D-glucose, D-xylose, L-arabinose, D-galactose, or D-galacturonic acid as acceptor substrate or with UTP, CTP, GTP, UDP, and ADP as donor substrates
the enzyme is absolutely specific for D-glucuronate and ATP, no activity with D-glucose, D-xylose, L-arabinose, D-galactose, or D-galacturonic acid as acceptor substrate or with UTP, CTP, GTP, UDP, and ADP as donor substrates
the enzyme is absolutely specific for D-glucuronate and ATP, no activity with D-glucose, D-xylose, L-arabinose, D-galactose, or D-galacturonic acid as acceptor substrate or with UTP, CTP, GTP, UDP, and ADP as donor substrates
no activity with CTP, GTP, TTP, dATP, dCTP, dGTP and dTTP, D-glucose, D-xylose, L-arabinose, L-fucose, D-mannose, L-rhamnose, D-galactose, and D-galacturonate
in Arabidopsis, the enzyme is expressed in all tissues with a preference for pollen. The expresseion level of glucuronokinase 1 is about 15fold higher than of glucuronokinase 2
in Arabidopsis, the enzyme is expressed in all tissues with a preference for pollen. The expresseion level of glucuronokinase 1 is about 15fold higher than of glucuronokinase 2
in Arabidopsis, the enzyme is expressed in all tissues with a preference for pollen. The expresseion level of glucuronokinase 1 is about 15fold higher than of glucuronokinase 2
in Arabidopsis, the enzyme is expressed in all tissues with a preference for pollen. The expression level of glucuronokinase 1 is about 15fold higher than of glucuronokinase 2
in Arabidopsis, the enzyme is expressed in all tissues with a preference for pollen. The expression level of glucuronokinase 1 is about 15fold higher than of glucuronokinase 2
in Arabidopsis, the enzyme is expressed in all tissues with a preference for pollen. The expression level of glucuronokinase 1 is about 15fold higher than of glucuronokinase 2
the transcripts abundance of isozyme AtGALK2 is much less than that of isozyme AtGlcAK in all analyzed tissues. Except for the transcripts in seed, the expression abundance of AtGALK2 in the rest of detected tissues is always less than half the level of AtGlcAK
the transcripts abundance of isozyme AtGALK2 is much less than that of isozyme AtGlcAK in all analyzed tissues. Except for the transcripts in seed, the expression abundance of AtGALK2 in the rest of detected tissues is always less than half the level of AtGlcAK
the transcripts abundance of isozyme AtGALK2 is much less than that of isozyme AtGlcAK in all analyzed tissues. Except for the transcripts in seed, the expression abundance of AtGALK2 in the rest of detected tissues is always less than half the level of AtGlcAK
there is reduced primary root elongation and lateral root formation in atglcak mutants under osmotic stress. The atglcak mutants display enhanced stomatal opening in response to abscisic acid (ABA), elevated water loss, and impaired drought tolerance. Under water stress, the accumulation of reducing and soluble sugars is reduced in atglcak mutants, and the metabolism of glucose and sucrose is affected by the synthetic pathway of UDP-GlcA. Furthermore, a reduced level of starch in atglcak mutants is observed under normal conditions
there is reduced primary root elongation and lateral root formation in atglcak mutants under osmotic stress. The atglcak mutants display enhanced stomatal opening in response to abscisic acid (ABA), elevated water loss, and impaired drought tolerance. Under water stress, the accumulation of reducing and soluble sugars is reduced in atglcak mutants, and the metabolism of glucose and sucrose is affected by the synthetic pathway of UDP-GlcA. Furthermore, a reduced level of starch in atglcak mutants is observed under normal conditions
the enzyme is involved in the the myo-inositol oxygenase pathway to nucleotide sugars, as part of the nucleotide sugar interconversion pathway, overview
the enzyme is involved in the the myo-inositol oxygenase pathway to nucleotide sugars, as part of the nucleotide sugar interconversion pathway, overview
one alternative pathway for ascorbic acid (AsA) synthesis, which is similar to the biosynthesis route in mammals, is controversially discussed for plants. Pathway overview for AsA biosynthesis in plants, overview. Here, myo-inositol is cleaved to glucuronic acid and then converted via L-gulonate to AsA. In contrast to animals, plants have an effective recycling pathway for glucuronic acid, being a competitor for the metabolic rate. Recycling involves a phosphorylation at C1 by the enzyme glucuronokinase
one alternative pathway for ascorbic acid (AsA) synthesis, which is similar to the biosynthesis route in mammals, is controversially discussed for plants. Pathway overview for AsA biosynthesis in plants, overview. Here, myo-inositol is cleaved to glucuronic acid and then converted via L-gulonate to AsA. In contrast to animals, plants have an effective recycling pathway for glucuronic acid, being a competitor for the metabolic rate. Recycling involves a phosphorylation at C1 by the enzyme glucuronokinase
nucleotide sugars are building blocks for carbohydrate polymers in plant cell walls, the main precursor for primary cell walls is UDP-glucuronic acid, which can be synthesized via two independent pathways. One starts with the ring cleavage of myo-inositol into glucuronic acid, which requires a glucuronokinase and a pyrophosphorylase for activation into UDP-glucuronate
nucleotide sugars are building blocks for carbohydrate polymers in plant cell walls, the main precursor for primary cell walls is UDP-glucuronic acid, which can be synthesized via two independent pathways. One starts with the ring cleavage of myo-inositol into glucuronic acid, which requires a glucuronokinase and a pyrophosphorylase for activation into UDP-glucuronate
isozyme glucuronokinase 1 gene, AtGlcAK, in Arabidopsis thaliana is involved in drought tolerance by modulating sugar metabolism and in abscisic acid-mediated stomata movement. AtGlcAK is implicated in carbohydrate metabolism and regulates the synthesis of glucose and sucrose which served as signaling molecules to regulate gene expressions, development and adaption to environmental stress factors
isozyme glucuronokinase 1 gene, AtGlcAK, in Arabidopsis thaliana is involved in drought tolerance by modulating sugar metabolism and in abscisic acid-mediated stomata movement. AtGlcAK is implicated in carbohydrate metabolism and regulates the synthesis of glucose and sucrose which served as signaling molecules to regulate gene expressions, development and adaption to environmental stress factors
generation of a frameshift mutations in the major expressed isoform glucuronokinase 1 to potentially redirect metabolites to ascorbic acid (AsA). But radiotracer experiments with 3H-myo-inositol reveal that the mutants in glucuronokinase 1 accumulate only glucuronic acid and incorporate less metabolite into cell wall polymers. AsA is not labelled, suggesting that Arabidopsis thaliana cannot efficiently use glucuronic acid for AsA biosynthesis. All four mutants in glucuronokinase as well as the wild type have the same level of AsA in leaves. Construction of CRISPR/Cas9 knockouts, mutants of GlcAK1 are generated using CRISPR/Cas9 technology including transformation by the Agrobacterium tumefaciens (strain GV3101) system, resulting in mutants glcak1-3 and glcak1-4. Analysis of T-DNA insertional mutants glcak1-1 and glcak1-2
identification of two T-DNA insertion homozygous mutants of AtGlcAK, atglcak-1 and atglcak-2. AtGlcAK mutants display hypersensitivity to abscisic acid (ABA) and reduced root development under water stress, rendering the plants more susceptible to drought stress. The atglcak mutants display shorter primary root and less lateral roots under osmotic stress. Loss-of-function of AtGlcAK Affects the expression of ABA-induced and sugar-metabolic genes. Phenotypes of atglcak-1 and atglcak-2, overview
identification of two T-DNA insertion homozygous mutants of AtGlcAK, atglcak-1 and atglcak-2. AtGlcAK mutants display hypersensitivity to abscisic acid (ABA) and reduced root development under water stress, rendering the plants more susceptible to drought stress. The atglcak mutants display shorter primary root and less lateral roots under osmotic stress. Loss-of-function of AtGlcAK Affects the expression of ABA-induced and sugar-metabolic genes. Phenotypes of atglcak-1 and atglcak-2, overview
generation of a frameshift mutations in the major expressed isoform glucuronokinase 1 to potentially redirect metabolites to ascorbic acid (AsA). But radiotracer experiments with 3H-myo-inositol reveal that the mutants in glucuronokinase 1 accumulate only glucuronic acid and incorporate less metabolite into cell wall polymers. AsA is not labelled, suggesting that Arabidopsis thaliana cannot efficiently use glucuronic acid for AsA biosynthesis. All four mutants in glucuronokinase as well as the wild type have the same level of AsA in leaves. Construction of CRISPR/Cas9 knockouts, mutants of GlcAK1 are generated using CRISPR/Cas9 technology including transformation by the Agrobacterium tumefaciens (strain GV3101) system, resulting in mutants glcak1-3 and glcak1-4. Analysis of T-DNA insertional mutants glcak1-1 and glcak1-2
identification of two T-DNA insertion homozygous mutants of AtGlcAK, atglcak-1 and atglcak-2. AtGlcAK mutants display hypersensitivity to abscisic acid (ABA) and reduced root development under water stress, rendering the plants more susceptible to drought stress. The atglcak mutants display shorter primary root and less lateral roots under osmotic stress. Loss-of-function of AtGlcAK Affects the expression of ABA-induced and sugar-metabolic genes. Phenotypes of atglcak-1 and atglcak-2, overview
native enzyme 688.5fold from pollen by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration followed by ultrafiltration, affinity chromatography, and another step of anion exchange chromatography, and hydrophobic interaction chromatography
cloning from Arabidopsis thaliana using the peptide sequences of Lillium longiflorum enzyme, DNA and amino acid sequence determination and analysis, expression as His6-tagged protein in Escherichia coli strain XL-1 Blue
Nonradioactive enzyme measurement by high-performance liquid chromatography of partially purified sugar-1-kinase (glucuronokinase) from pollen of Lilium longiflorum