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3'-phosphoadenylyl sulfate + Ac-YLFSVHWPPLNK(COOSu)A-OH
adenosine 3',5'-bisphosphate + Ac-Y(O-sulfate)LFSVHWPPLNK(COOSu)A-OH
-
an N-succinimidyl carbamate modified peptide substrate
-
-
?
3'-phosphoadenylyl sulfate + Ac-YLFSVHWPPLNKA-OH
adenosine 3',5'-bisphosphate + Ac-Y(O-sulfate)LFSVHWPPLNKA-OH
-
-
-
-
?
3'-phosphoadenylyl sulfate + benzyl-Glu-Tyr
adenosine 3',5'-bisphosphate + benzyl-Glu-Tyr-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + C3aR tyrosine
adenosine 3',5'-bisphosphate + C3aR tyrosine O-sulfate
-
C3a-anaphylatoxin chemotactic receptor,C3aR. Sulfation sequence motif is HNRCGY174KFGLSSSLDY184PDFY188 GDPLEQGFQDY317Y318NLGQF
-
-
?
3'-phosphoadenylyl sulfate + C4P5Y5 peptide
adenosine 3',5'-bisphosphate + sulfated C4P5Y5 peptide
a C4 complement derived peptide substrate, sulfation of the Tyr741 residue
-
-
?
3'-phosphoadenylyl sulfate + C5aR tyrosine
adenosine 3',5'-bisphosphate + C5aR tyrosine O-sulfate
-
C5a-anaphylatoxin chemotactic receptor. Sulfation sequence motif is TTPDY11GHY14DDKDTLD
-
-
?
3'-phosphoadenylyl sulfate + CC receptor tyrosine
adenosine 3',5'-bisphosphate + CC receptor tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + CCR5 1-18 peptide
adenosine 3',5'-bisphosphate + sulfated CCR5 1-18peptide
-
substrate is a synthetic human CCR5 peptide MDYQVSSPIYDINYYTSE-NH2. Sulfation pattern: Tyr3 is sulfated first, followed by Tyr14 or Tyr15, and finally Tyr10
-
-
?
3'-phosphoadenylyl sulfate + CCR5 2-18 peptide
adenosine 3',5'-bisphosphate + sulfated CCR5 2-18peptide
-
substrate is a synthetic human CCR5 peptide DYQVSSPIYDINYYTSE-NH2. Sulfation pattern: Tyr14 and Tyr15 are sulfated first, followed by Tyr10, with Tyr3 sulfated last
-
-
?
3'-phosphoadenylyl sulfate + CCR8 1-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 1-17 M1A peptide
3'-phosphoadenylyl sulfate + CCR8 2-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 2-17 M1A peptide
3'-phosphoadenylyl sulfate + cholecystokinin I
?
-
-
-
-
?
3'-phosphoadenylyl sulfate + cholecystokinin-2
?
-
-
-
-
?
3'-phosphoadenylyl sulfate + cholecystokinin-3
?
-
-
-
-
?
3'-phosphoadenylyl sulfate + CX3CR1 tyrosine
adenosine 3',5'-bisphosphate + CX3CR1 tyrosine O-sulfate
3'-phosphoadenylyl sulfate + CXC-chemokine receptor 4
adenosine 3',5'-bisphosphate + ?
3'-phosphoadenylyl sulfate + CXCR4 1-38
adenosine 3',5'-bisphosphate + ?
CC-chemokine receptor 4 peptide, amino acids 1-38
the enzyme has a strong site preference for sulfation of Tyr-21 over Tyr-7 and Tyr-12, sulfation of Tyr-21 is required for high-affinity interaction with stromal cell-derived factor-1 (SDF-1alpha)
-
?
3'-phosphoadenylyl sulfate + CXCR4 peptide tyrosine
adenosine 3',5'-bisphosphate + CXCR4 peptide tyrosine O-sulfate
-
a cytokine receptor peptide fragment
-
-
?
3'-phosphoadenylyl sulfate + EAY
adenosine 3',5'-bisphosphate + EAY(O-sulfate)
-
-
-
-
?
3'-phosphoadenylyl sulfate + EDFEDYEFDG
adenosine 3',5'-bisphosphate + EDFEDY(sulfated)EFDG
GST-tagged CC4-tide, peptide 5-FAM-EDFEDYEFDG-CONH2, from human complement C4 protein
-
-
?
3'-phosphoadenylyl sulfate + EDYDTTTEFDsYGD
adenosine 3',5'-bisphosphate + EDsYDTTTEFDsYGD
G protein-coupled receptor, human chemokine receptor 1, amino acids 8-20
-
-
?
3'-phosphoadenylyl sulfate + EDYDTTTEFDYGD
adenosine 3',5'-bisphosphate + EDYDTTTEFDsYGD
G protein-coupled receptor human chemokine receptor 1, amino acids 8-20
-
-
?
3'-phosphoadenylyl sulfate + EEPEYGE
adenosine 3',5'-bisphosphate + EEPEY(O-sulfate)GE
-
-
-
-
?
3'-phosphoadenylyl sulfate + ENYSYDLDYYS
adenosine 3',5'-bisphosphate + ENsYSsYDLDsYsYS
G protein-coupled receptor human gpr1, amino acids 13-23, sulfation order: first Tyr21, second Tyr22, third Tyr15 or 17, fourth Tyr 15 or 17
-
-
?
3'-phosphoadenylyl sulfate + ERHTRSYDYMEGGD
adenosine 3',5'-bisphosphate + ERHTRSYDY(sulfated)MEGGD
FGF7-tide, peptide 5-FAM-ERHTRSYDYMEGGD-CONH2, from fibroblast growth factor 7
-
-
?
3'-phosphoadenylyl sulfate + FSHR tyrosine
adenosine 3',5'-bisphosphate + FSHR tyrosine O-sulfate
-
follicle-stimulating hormone receptor, FSHR. Sulfation sequence motif is F-D/E-Y335
-
-
?
3'-phosphoadenylyl sulfate + gastrin peptide
adenosine 3',5'-bisphosphate + sulfated gastrin peptide
-
-
-
?
3'-phosphoadenylyl sulfate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + human anionic trypsinogen tyrosine
adenosine 3',5'-bisphosphate + trypsinogen tyrosine O-sulfate
i.e. serine protease 2, sulfation of Tyr154 facilitated by a unique sequence context which is characteristically found in primate trypsinogens
-
-
?
3'-phosphoadenylyl sulfate + LHR/CGR tyrosine
adenosine 3',5'-bisphosphate + LHR/CGR tyrosine O-sulfate
-
luteinising hormone receptor, LHR/chorionic gonadotropin receptor, CGR. Sulfation sequence motif is Y385-D/E-Y
-
-
?
3'-phosphoadenylyl sulfate + Mfge8 protein tyrosine
adenosine 3',5'-bisphosphate + Mfge8 protein tyrosine O-sulfate
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
-
i.e. ATEYEYLDYDFL
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 tyrosine
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
3'-phosphoadenylyl sulfate + peptide C4P5Y3
?
-
-
-
?
3'-phosphoadenylyl sulfate + peptide EAY
adenosine 3',5'-bisphosphate + peptide EAY(O-sulfate)
3'-phosphoadenylyl sulfate + peptide Hir-(57-65)
?
3'-phosphoadenylyl sulfate + peptide tub-1
?
-
-
-
-
?
3'-phosphoadenylyl sulfate + peptide Y+1 tyrosine
adenosine 3',5'-bisphosphate + peptide Y+1 tyrosine-O-sulfate
3'-phosphoadenylyl sulfate + phytosulfokine-alpha tyrosine
adenosine 3',5'-bisphosphate + phytosulfokine-alpha tyrosine-O-sulfate
3'-phosphoadenylyl sulfate + poly-Glu6Ala3Tyr1 peptide
adenosine 3',5'-bisphosphate + poly-Glu6Ala3Tyrsulfate peptide
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
3'-phosphoadenylyl sulfate + PSGL-1
adenosine 3',5'-bisphosphate + PSGL-1 sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + PSGL-1 1-15 peptide
adenosine 3',5'-bisphosphate + sulfated PSGL-1 1-15 peptide
QATEYEYLDYDFLPE-NH2
-
-
?
3'-phosphoadenylyl sulfate + PSGL-1 protein
adenosine 3',5'-bisphosphate + PSGL-1 protein tyrosine-O-sulfate
-
i.e. ATEYEYLDYDFL
-
-
?
3'-phosphoadenylyl sulfate + QATEYEYLDYDFLPE
adenosine 3',5'-bisphosphate + QATEsYEsYLDsYDFLPE
human PSGL-1, amino acids 42-56, sulfation order: first Tyr51, second Tyr46 or 48, third Tyr46 or 48
-
-
?
3'-phosphoadenylyl sulfate + QATEYEYLDYDFLPE-NH2
adenosine 3',5'-bisphosphate + ?
P-selectin glycoprotein ligand-1, amino acids 1-15
-
-
?
3'-phosphoadenylyl sulfate + recombinant hirudin HV2
?
-
-
-
-
?
3'-phosphoadenylyl sulfate + RNase 9 protein tyrosine
adenosine 3',5'-bisphosphate + RNase 9 protein tyrosine O-sulfate
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine
adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
3'-phosphoadenylyl sulfate + root meristem growth factor 7 tyrosine
adenosine 3',5'-bisphosphate + root meristem growth factor 7 tyrosine-O-sulfate
3'-phosphoadenylyl sulfate + S1P1R tyrosine
adenosine 3',5'-bisphosphate + S1P1R tyrosine O-sulfate
-
type 1 Sphingosine 1-phosphate receptor, S1P1R. Sulfation sequence motif is D-Y19-V/G/-N-Y22-D
-
-
?
3'-phosphoadenylyl sulfate + statherin
adenosine 3',5'-bisphosphate + statherin O-sulfate
3'-phosphoadenylyl sulfate + statherin
adenosine 3',5'-bisphosphate + statherin-O-sulfate
-
a 43-residue polypeptide, which is tyrosine-rich and contains 7 tyrosine residues
-
-
?
3'-phosphoadenylyl sulfate + SYGDEYPDYLD
adenosine 3',5'-bisphosphate + SsYGDEsYPDsYLD
G protein-coupled receptor human dez, amino acids 13-23, sulfation order: first Tyr21, second Tyr18, third Tyr14
-
-
?
3'-phosphoadenylyl sulfate + TEYEYLDYDFLPETE
adenosine 3',5'-bisphosphate + TEYEYLDY(sulfated)DFLPETE
PSGL1-tide, peptide 5-FAM-TEYEYLDYDFLPETE-CONH2, from P-selectin glycoprotein ligand-1
-
-
?
3'-phosphoadenylyl sulfate + trypsinogen tyrosine
adenosine 3',5'-bisphosphate + trypsinogen tyrosine O-sulfate
sulfation of Tyr154 with Asp as important determinant. Mutation D153N, a naturally occuring polymorphisms in African population, results in a complete loss of trypsinogen sulfation, while mutation of Gly151 and Pro155 of the trypsinogen has no effect on enzyme activity. Genotyping of different populations for the substrate polymorphism, overview
-
-
?
3'-phosphoadenylyl sulfate + TSH receptor
adenosine 3',5'-bisphosphate + TSH receptor O-sulfate
3'-phosphoadenylyl sulfate + TSHR tyrosine
adenosine 3',5'-bisphosphate + TSHR tyrosine O-sulfate
-
thyroid-stimulating hormone receptor, TSHR. Sulfation sequence motif is Y385-D/E-Y
-
-
?
3'-phosphoadenylyl sulfate + TTFFDsYDYGA
adenosine 3',5'-bisphosphate + TTFFDsYDsYGA
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
-
-
?
3'-phosphoadenylyl sulfate + TTFFDYDsYGA
adenosine 3',5'-bisphosphate + TTFFDsYDsYGA
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
-
-
?
3'-phosphoadenylyl sulfate + TTFFDYDYGA
adenosine 3',5'-bisphosphate + TTFFDsYDYGA
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
-
-
?
3'-phosphoadenylyl sulfate + TTFFDYDYGA
adenosine 3',5'-bisphosphate + TTFFDYDsYGA
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
-
-
?
3'-phosphoadenylyl sulfate + TTVTDYYYPDIFSS
adenosine 3',5'-bisphosphate + TTVTDY(sulfated)YYPDIFSS
CCR8-tide, peptide 5-FAM-TTVTDYYYPDIFSS-CONH2, from C-C motif chemokine receptor 8
-
-
?
3'-phosphoadenylyl sulfate + Tyr-Tyr-Tyr
adenosine 3',5'-bisphosphate + Tyr-Tyr-Tyr-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + vitronectin peptide
adenosine 3',5'-bisphosphate + vitronectin-O-sulfate
amino acids 75-91, Y(SO3H)TVY(SO3H)DDGEEKNDATVHE
-
-
?
3'-phosphoadenylyl sulfate + VTDsYsYYPDI
adenosine 3',5'-bisphosphate + VTDsYsYsYPDI
Tyr16-sulfated G protein-coupled receptor CCR8, amino acids 12-20
-
-
?
3'-phosphoadenylyl sulfate + VTDsYYsYPDI
adenosine 3',5'-bisphosphate + VTDsYsYsYPDI
CC-chemokine receptor 8 nonapeptide, sY15CCR8
-
-
?
3'-phosphoadenylyl sulfate + VTDsYYYPDG
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
CC-chemokine receptor 8 nonapeptide, sY15sY17CCR8
-
-
?
3'-phosphoadenylyl sulfate + VTDsYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
3'-phosphoadenylyl sulfate + VTDYsYYPDI
adenosine 3',5'-bisphosphate + VTDsYsYYPDI
Tyr16-sulfated G protein-coupled receptor CCR8, amino acids 12-20
-
-
?
3'-phosphoadenylyl sulfate + VTDYYsYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
both isoenzymes sequentially sulfate the CCR8 peptide such that Tyr15 is sulfated first followed by Tyr17 sulfation
-
-
?
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYYPDI
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDYYsYPDI
a peptide modeled on the N-terminal extracellular domain of human CCR8, amino acids 12-20
-
-
?
3'-phosphoadenylyl sulfate + [PSGL-1 peptide]-L-tyrosine
adenosine 3',5'-bisphosphate + [PSGL-1 peptide]-L-tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + [Y+3 peptide]-L-tyrosine
adenosine 3',5'-bisphosphate + [Y+3 peptide]-L-tyrosine-O-sulfate
i.e. biotin-RDRRQATEYEYLDYDFLPETEPPP
-
-
?
3'-phosphoadenylyl-sulfate + ATEFEFLDYDFL
adenosine 3',5'-bisphosphate + ?
-
-
-
?
3'-phosphoadenylyl-sulfate + ATEFEYLDYDFL
adenosine 3',5'-bisphosphate + ?
-
-
-
?
3'-phosphoadenylyl-sulfate + ATEYEFLDYDFL
adenosine 3',5'-bisphosphate + ?
-
-
-
?
3'-phosphoadenylyl-sulfate + ATEYEYLDYDFL
adenosine 3',5'-bisphosphate + ?
-
-
-
?
3'-phosphoadenylyl-sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
TPST-A is involved in the cuticle formation in the nematode. TPST-A RNAi significantly decreases the dityrosine level in the worms, raising the possibility that the sulfation process may be a pre-requisite for the collagen tyrosine cross-linking
-
-
?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
3'-phosphoadenylylsulfate + alpha-tubulin
adenosine 3',5'-bisphosphate + ?
-
peptide PKV
-
-
?
3'-phosphoadenylylsulfate + C-terminal peptide fragments of complement component C4
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + C4 alpha chain
adenosine 3',5'-bisphosphate + ?
-
more efficiently sulphated by TPST1 than TPST2
-
-
?
3'-phosphoadenylylsulfate + DYIYTQDVK
adenosine 3',5'-bisphosphate + ?
3'-phosphoadenylylsulfate + DYQVSSPIYDINYYTSE
adenosine 3',5'-bisphosphate + ?
-
amino acids 2-18 derived from peptide CC-chemokine receptor 5, final product has four sulfotyrosine residues, sulfation occurs in the following order: Tyr13, Tyr14, Tyr9, Tyr2
-
-
?
3'-phosphoadenylylsulfate + EANEDFEDYEFDE
adenosine 3',5'-bisphosphate + ?
-
i.e. P395A
-
-
?
3'-phosphoadenylylsulfate + EEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
3'-phosphoadenylylsulfate + FHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
3'-phosphoadenylylsulfate + heparin cofactor II
adenosine 3',5'-bisphosphate + ?
-
more efficiently sulphated by TPST1 than TPST2
-
-
?
3'-phosphoadenylylsulfate + KKE(EPEYGE)2
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + KKE(EPEYGE)3
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + KKEEPEYGE
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + KKEEPEYGE(EPEFGE)2
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + KKSAEDYEYPSMG
adenosine 3',5'-bisphosphate + ?
3'-phosphoadenylylsulfate + KKSAEDYQYPSMG
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + KKSAENYEYPSMG
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + KKSAENYQYPSMG
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + PKG
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + preprocholecystokinin
adenosine 3',5'-bisphosphate + ?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPE
adenosine 3',5'-bisphosphate + ?
-
derived from N-terminal residues of the mature leukocyte adhesion molecule P-selectin glycoprotein ligand 1
-
-
?
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPEC
adenosine 3',5'-bisphosphate + ?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
3'-phosphoadenylylsulfate + tert-butoxycarbonylcholecystokinin octapeptide
adenosine 3',5'-bisphosphate + ?
-
BOC-CCK
-
-
?
3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
additional information
?
-
3'-phosphoadenylyl sulfate + CCR8 1-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 1-17 M1A peptide
-
substrate is a synthetic modified mouse CCR8 peptide ADYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr3 is sulfated first, followed by Tyr14, with Tyr15 last
-
-
?
3'-phosphoadenylyl sulfate + CCR8 1-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 1-17 M1A peptide
-
substrate is a synthetic modified mouse CCR8 peptide ADYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr3 is sulfated first, followed by Tyr14, with Tyr15 last
-
-
?
3'-phosphoadenylyl sulfate + CCR8 2-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 2-17 M1A peptide
-
substrate is a synthetic modified mouse CCR8 peptide DYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr14 is sulfated first, followed by Tyr3 and Tyr15
-
-
?
3'-phosphoadenylyl sulfate + CCR8 2-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 2-17 M1A peptide
-
substrate is a synthetic modified mouse CCR8 peptide DYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr14 is sulfated first, followed by Tyr3 and Tyr15
-
-
?
3'-phosphoadenylyl sulfate + CX3CR1 tyrosine
adenosine 3',5'-bisphosphate + CX3CR1 tyrosine O-sulfate
-
a cytokine receptor for CXCL10/IP-10 and CXCL11/I-TAC, contains two potential tyrosine sulfation sites within the sequence ENFSSSY27DY29GENESDS
-
-
?
3'-phosphoadenylyl sulfate + CX3CR1 tyrosine
adenosine 3',5'-bisphosphate + CX3CR1 tyrosine O-sulfate
-
a cytokine receptor for CXCL10/IP-10 and CXCL11/I-TAC, contains two potential tyrosine sulfation sites within the sequence ENFSSSY27DY29GENESDS. Determination of sulfation sites by mutational analyis, overview. CX3CR1 has one known chemokine ligand, fractalkine and two tyrosine residues in its N-terminal region, Tyr14 and Tyr22
-
-
?
3'-phosphoadenylyl sulfate + CXC-chemokine receptor 4
adenosine 3',5'-bisphosphate + ?
CXCR4 signaling is indispensable for embryonic development
-
-
?
3'-phosphoadenylyl sulfate + CXC-chemokine receptor 4
adenosine 3',5'-bisphosphate + ?
CXCR4
-
-
?
3'-phosphoadenylyl sulfate + Mfge8 protein tyrosine
adenosine 3',5'-bisphosphate + Mfge8 protein tyrosine O-sulfate
-
the long isoform of Mfge8, substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview
-
-
?
3'-phosphoadenylyl sulfate + Mfge8 protein tyrosine
adenosine 3',5'-bisphosphate + Mfge8 protein tyrosine O-sulfate
-
Mfge8 is expressed on mouse sperm, and recombinantly in Escherichia coli strain BL21(DE3) as His-tagged protein
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1
?
recombinantly expressed substrate PSGL-1, glycoprotein found on the plasma membrane of neutrophils or monocytes. TPST activity on GST-tagged PSGL-1 is not affected by the GST fusion tag
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1
?
recombinantly expressed substrate PSGL-1, glycoprotein found on the plasma membrane of neutrophils or monocytes. TPST activity on GST-tagged PSGL-1 is not affected by the GST fusion tag
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 tyrosine
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
-
sulfoTyr plays a functional role is P-selectin glycoprotein ligand-1, which is involved in leukocyte-mediated inflammation
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 tyrosine
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
-
tyrosine sulfation of the amino-terminal region, structural basis of sulfotyrosine recognition, overview
-
-
?
3'-phosphoadenylyl sulfate + peptide EAY
adenosine 3',5'-bisphosphate + peptide EAY(O-sulfate)
-
-
-
-
?
3'-phosphoadenylyl sulfate + peptide EAY
adenosine 3',5'-bisphosphate + peptide EAY(O-sulfate)
-
-
-
-
?
3'-phosphoadenylyl sulfate + peptide Hir-(57-65)
?
-
-
-
-
?
3'-phosphoadenylyl sulfate + peptide Hir-(57-65)
?
-
-
-
-
?
3'-phosphoadenylyl sulfate + peptide Y+1 tyrosine
adenosine 3',5'-bisphosphate + peptide Y+1 tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + peptide Y+1 tyrosine
adenosine 3',5'-bisphosphate + peptide Y+1 tyrosine-O-sulfate
i.e. peptide Glu-Leu-Asp-Tyr-Dap-Gly-Glu-Ala
-
-
?
3'-phosphoadenylyl sulfate + phytosulfokine-alpha tyrosine
adenosine 3',5'-bisphosphate + phytosulfokine-alpha tyrosine-O-sulfate
PSK-alpha
-
-
?
3'-phosphoadenylyl sulfate + phytosulfokine-alpha tyrosine
adenosine 3',5'-bisphosphate + phytosulfokine-alpha tyrosine-O-sulfate
PSK-alpha
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
sulfation of protein in the retina, identification of protein substrates, sulfation pattern, overview
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
sulfation of protein in the retina, identification of protein substrates, sulfation pattern, overview
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
high activity, isozyme TPST-2
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
low activity, isozyme TPST-1
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
TPST is responsible for the sulfation of a variety of secretory and membrane proteins
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
tyrosine O-sulfation is a key post-translational modification that regulates protein-protein interactions in extracellular space
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
CC-chemokine receptor 8 nonapeptide, sY15CCR8 I20G mutant
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
tyrosine sulfonation of TSHR by TPST is crucial for thyrotropin signaling, overview
-
-
?
3'-phosphoadenylyl sulfate + RNase 9 protein tyrosine
adenosine 3',5'-bisphosphate + RNase 9 protein tyrosine O-sulfate
-
substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview
-
-
?
3'-phosphoadenylyl sulfate + RNase 9 protein tyrosine
adenosine 3',5'-bisphosphate + RNase 9 protein tyrosine O-sulfate
-
RNase 9 is a catalytically inactive RNase A family member of unknown function and is expressed only in the epididymis after onset of sexual maturity
-
-
?
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine
adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro
-
-
?
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine
adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro
-
-
?
3'-phosphoadenylyl sulfate + root meristem growth factor 7 tyrosine
adenosine 3',5'-bisphosphate + root meristem growth factor 7 tyrosine-O-sulfate
RGF7
-
-
?
3'-phosphoadenylyl sulfate + root meristem growth factor 7 tyrosine
adenosine 3',5'-bisphosphate + root meristem growth factor 7 tyrosine-O-sulfate
RGF7
-
-
?
3'-phosphoadenylyl sulfate + statherin
adenosine 3',5'-bisphosphate + statherin O-sulfate
-
TPST is responsible for the sulfation of a variety of secretory and membrane proteins, e.g. of the salivary secretory protein, statherin
-
-
?
3'-phosphoadenylyl sulfate + statherin
adenosine 3',5'-bisphosphate + statherin O-sulfate
-
determination of sulfation on a tyrosyl residue, probably in the sequence E-Q-P-L-Y, residues 26-30
-
-
?
3'-phosphoadenylyl sulfate + TSH receptor
adenosine 3',5'-bisphosphate + TSH receptor O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + TSH receptor
adenosine 3',5'-bisphosphate + TSH receptor O-sulfate
-
tyrosine sulfonation of TSHR by TPST2 is crucial for thyrotropin signaling, TPST1 and TPST2 mutant H266Q are incapable of mediating the signal transduction, overview
-
-
?
3'-phosphoadenylyl sulfate + VTDsYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
-
-
-
-
ir
3'-phosphoadenylyl sulfate + VTDsYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
CC-chemokine receptor 8 nonapeptide, amino acids 12-20
-
-
?
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYYPDI
-
-
-
-
ir
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYYPDI
non CC-chemokine receptor 8
-
-
?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
-
i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate
-
-
?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
-
i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate
-
-
?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
-
i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate
-
-
?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
-
i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate
-
-
?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
-
i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate
645804, 645805, 645807, 645812, 645814, 645816, 645819, 645820, 645821, 645825, 645826, 645828 -
-
?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
specifically sulfates Tyr-residues adjacent to acidic amino acids
-
-
?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
dominant characteristic is that there are generally between 3 and 4 acidic amino acids within the +/-5 residues of the sulfotyrosine
-
-
?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
-
-
-
?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
dominant characteristic is that there are generally between 3 and 4 acidic amino acids within the +/-5 residues of the sulfotyrosine
-
-
?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
polypeptides from PC12 cells, referred to as p113, p105, p86 and p84, according to their average MW
-
?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
specifically sulfates Tyr-residues adjacent to acidic amino acids
-
?
3'-phosphoadenylylsulfate + DYIYTQDVK
adenosine 3',5'-bisphosphate + ?
-
PP-PSK-(79-87), truncated sequence of preprophytosulfokinase, poor substrate
-
-
?
3'-phosphoadenylylsulfate + DYIYTQDVK
adenosine 3',5'-bisphosphate + ?
-
PP-PSK-(79-87), truncated sequence of preprophytosulfokinase, poor substrate
-
-
?
3'-phosphoadenylylsulfate + EEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
-
PP-PSK-(74-87), truncated sequence of preprophytosulfokinase
-
-
?
3'-phosphoadenylylsulfate + EEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
-
PP-PSK-(74-87), truncated sequence of preprophytosulfokinase
-
-
?
3'-phosphoadenylylsulfate + FHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
-
PP-PSK-(76-87), truncated sequence of preprophytosulfokinase, poor substrate
-
-
?
3'-phosphoadenylylsulfate + FHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
-
PP-PSK-(76-87), truncated sequence of preprophytosulfokinase, poor substrate
-
-
?
3'-phosphoadenylylsulfate + KKSAEDYEYPSMG
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + KKSAEDYEYPSMG
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + preprocholecystokinin
adenosine 3',5'-bisphosphate + ?
-
CCK-(107-115) corresponding to the carboxy-terminal sulfation sites of the protein
-
-
?
3'-phosphoadenylylsulfate + preprocholecystokinin
adenosine 3',5'-bisphosphate + ?
-
CCK-(107-115) corresponding to the carboxy-terminal sulfation sites of the protein
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
enzyme of trans-Golgi network catalyzing post-translational sulfation of a variety of secretory and membrane proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
-
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
involved in post-translational processing of specific PC12 cell proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPEC
adenosine 3',5'-bisphosphate + ?
derived from N-terminal residues of the mature leukocyte adhesion molecule P-selectin glycoprotein ligand 1
-
-
?
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPEC
adenosine 3',5'-bisphosphate + ?
derived from N-terminal residues of the mature leukocyte adhesion molecule P-selectin glycoprotein ligand 1
-
-
?
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPEC
adenosine 3',5'-bisphosphate + ?
-
derived from N-terminal residues of the mature leukocyte adhesion molecule P-selectin glycoprotein ligand 1
-
-
?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
-
rHV2 and Hir-(57-65) corresponding to the nine C-terminal amino acids of hirudin
-
-
?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
-
HV2, previously referred to as HV2,Lys-47, or rHV-1
-
-
?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
-
sulfated at physiological sulfation site: Tyr-63
-
-
?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
-
C-terminal fragment Hir-(54-65)
-
-
?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
-
higher activity for those peptides with aspartyl residues on N-terminal side of Tyr-residue compared with glutamyl residues
-
-
?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
-
rHV2 and Hir-(57-65) corresponding to the nine C-terminal amino acids of hirudin
-
-
?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
-
sulfated at physiological sulfation site: Tyr-63
-
-
?
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
-
peptides modelled after known or putative Tyr-sulfation sites of chromogranin B, i.e. cholecystokinin precursor, peptides CCK-1 and variants, secretogranin I, peptides SgI-1 to SgI-4, vitronectin modeled after phosphorylation site of alpha-tubulin or autophosphorylation site of pp60src, pp60v-src(Tyr-416)
-
-
?
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
-
structure based on sequences surrounding the sulfated Tyr of naturally sulfated proteins
-
-
?
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
-
highly site-specific
-
-
?
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
-
structure based on sequences surrounding the sulfated Tyr of naturally sulfated proteins
-
-
?
3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
-
PP-PSK-(73-87), truncated sequence of preprophytosulfokinase
-
-
?
3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
-
PP-PSK-(73-87), truncated sequence of preprophytosulfokinase
-
-
?
3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
-
PP-PSK-(73-87), truncated sequence of preprophytosulfokinase
-
-
?
3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
-
PP-PSK-(73-87), truncated sequence of preprophytosulfokinase
-
-
?
3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
-
PP-PSK-(73-87), truncated sequence of preprophytosulfokinase
-
-
?
additional information
?
-
-
structural determinants for substrate specificity
-
-
?
additional information
?
-
localization of the potential sulfation sites of Escherichia coli proteins on a proteome microarray by using a 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase (recombinant hPAPSS-1, UniProt ID O43252)-coupled tyrosylprotein sulfotransferase (TPST) catalysis system that involves in situ PAPS generation and TPST catalysis. Among the 4256 Escherichia coli strain K12 proteins, 875 sulfated proteins are identified via antisulfotyrosine primary and Cy3-labeled antimouse secondary antibodies and are potential proteins subjected to tyrosine sulfation by the recombinant Drosophila melanogaster TPST (DmTPST) with human PAPSS-1. Method overview
-
-
-
additional information
?
-
activity with peptides encompassing tyrosine sulfation sites of chemokine receptors, CCR2, CCR3, CCR5, CCR8, cholecystokinin CCK, and complement C4, overview
-
-
?
additional information
?
-
activity with peptides encompassing tyrosine sulfation sites of chemokine receptors, CCR2, CCR3, CCR5, CCR8, cholecystokinin CCK, and complement C4, overview
-
-
?
additional information
?
-
-
activity with peptides encompassing tyrosine sulfation sites of chemokine receptors, CCR2, CCR3, CCR5, CCR8, cholecystokinin CCK, and complement C4, overview
-
-
?
additional information
?
-
-
sulfation patterns, overview
-
-
?
additional information
?
-
-
enzyme activity with CCR receptors, overview
-
-
?
additional information
?
-
a microfluidic assay is used to quantify real-time peptide sulfation by TPST1. No activity with peptide lacking the single acceptor tyrosine residue, 5-FAM-EDFEDFEFDG-CONH2
-
-
-
additional information
?
-
a microfluidic assay is used to quantify real-time peptide sulfation by TPST1. No activity with peptide lacking the single acceptor tyrosine residue, 5-FAM-EDFEDFEFDG-CONH2
-
-
-
additional information
?
-
a microfluidic assay is used to quantify real-time peptide sulfation by TPST2. No activity with peptide lacking the single acceptor tyrosine residue, 5-FAM-EDFEDFEFDG-CONH2
-
-
-
additional information
?
-
a microfluidic assay is used to quantify real-time peptide sulfation by TPST2. No activity with peptide lacking the single acceptor tyrosine residue, 5-FAM-EDFEDFEFDG-CONH2
-
-
-
additional information
?
-
development of a fluorescent peptide assay, termed TPST2/Y+1 peptide assay, for TPST1 and 2 activity, substrate screening using the LOPAC library in a high-throughput screening, overview
-
-
-
additional information
?
-
development of a fluorescent peptide assay, termed TPST2/Y+1 peptide assay, for TPST1 and 2 activity, substrate screening using the LOPAC library in a high-throughput screening, overview
-
-
-
additional information
?
-
in vitro sulfation of peptide substrates, analytical and semipreparative-scale peptide sulfation reactions
-
-
-
additional information
?
-
in vitro sulfation of peptide substrates, analytical and semipreparative-scale peptide sulfation reactions
-
-
-
additional information
?
-
in vitro sulfation of peptide substrates, analytical and semipreparative-scale peptide sulfation reactions, product analysis by NMR
-
-
-
additional information
?
-
in vitro sulfation of peptide substrates, analytical and semipreparative-scale peptide sulfation reactions, product analysis by NMR
-
-
-
additional information
?
-
synthetic fluorescent substrates, fluorescein-tagged hexasaccharide glycans, or FAM-labelled peptides derived from physiological target proteins are used in microfluidics-based mobility shift assay (MSA) to carry out real-time analysis of the enzyme kinetics of TPST 1/2
-
-
-
additional information
?
-
the structures of TPST1 complexed with two substrate peptides (C4P5Y5 derived from complement C4 and a gastrin peptide), that are catalysed by human TPST1 with significantly different efficiencies, reveal details about the binding modes found in the two complexes and into the sulfation mechanism for these substrates. The structures also contain only lumenal portion of the enzyme, without the N-terminus and the TM domain. The active site of human TPST1 recognizes the residue at the -3 position in the substrate through electrostatic and hydrophobic interactions. The enzyme shows broad substrate specificity
-
-
-
additional information
?
-
-
the structures of TPST1 complexed with two substrate peptides (C4P5Y5 derived from complement C4 and a gastrin peptide), that are catalysed by human TPST1 with significantly different efficiencies, reveal details about the binding modes found in the two complexes and into the sulfation mechanism for these substrates. The structures also contain only lumenal portion of the enzyme, without the N-terminus and the TM domain. The active site of human TPST1 recognizes the residue at the -3 position in the substrate through electrostatic and hydrophobic interactions. The enzyme shows broad substrate specificity
-
-
-
additional information
?
-
-
missense mutation of Tpst2 leads to the autosomal recessive, fetal-onset, severe thyroid hypoplasia phenotype related to TSH hyporesponsiveness in growth-retarded mice, overview
-
-
?
additional information
?
-
-
protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST1, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview
-
-
?
additional information
?
-
-
protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST2, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview
-
-
?
additional information
?
-
-
TPST-2 catalyzes post-translational protein tyrosine O-sulfation, TPST-2, but not TPST-1, deficiency causes male infertility
-
-
?
additional information
?
-
-
isozyme Tpst1 shows differences in the macromolecular substrate specificities, overview
-
-
?
additional information
?
-
-
isozyme Tpst2 shows differences in the macromolecular substrate specificities, overview
-
-
?
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3'-phosphoadenylyl sulfate + C3aR tyrosine
adenosine 3',5'-bisphosphate + C3aR tyrosine O-sulfate
-
C3a-anaphylatoxin chemotactic receptor,C3aR. Sulfation sequence motif is HNRCGY174KFGLSSSLDY184PDFY188 GDPLEQGFQDY317Y318NLGQF
-
-
?
3'-phosphoadenylyl sulfate + C5aR tyrosine
adenosine 3',5'-bisphosphate + C5aR tyrosine O-sulfate
-
C5a-anaphylatoxin chemotactic receptor. Sulfation sequence motif is TTPDY11GHY14DDKDTLD
-
-
?
3'-phosphoadenylyl sulfate + CC receptor tyrosine
adenosine 3',5'-bisphosphate + CC receptor tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + CX3CR1 tyrosine
adenosine 3',5'-bisphosphate + CX3CR1 tyrosine O-sulfate
-
a cytokine receptor for CXCL10/IP-10 and CXCL11/I-TAC, contains two potential tyrosine sulfation sites within the sequence ENFSSSY27DY29GENESDS
-
-
?
3'-phosphoadenylyl sulfate + CXC-chemokine receptor 4
adenosine 3',5'-bisphosphate + ?
CXCR4 signaling is indispensable for embryonic development
-
-
?
3'-phosphoadenylyl sulfate + FSHR tyrosine
adenosine 3',5'-bisphosphate + FSHR tyrosine O-sulfate
-
follicle-stimulating hormone receptor, FSHR. Sulfation sequence motif is F-D/E-Y335
-
-
?
3'-phosphoadenylyl sulfate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + LHR/CGR tyrosine
adenosine 3',5'-bisphosphate + LHR/CGR tyrosine O-sulfate
-
luteinising hormone receptor, LHR/chorionic gonadotropin receptor, CGR. Sulfation sequence motif is Y385-D/E-Y
-
-
?
3'-phosphoadenylyl sulfate + Mfge8 protein tyrosine
adenosine 3',5'-bisphosphate + Mfge8 protein tyrosine O-sulfate
-
the long isoform of Mfge8, substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
-
i.e. ATEYEYLDYDFL
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 tyrosine
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
-
sulfoTyr plays a functional role is P-selectin glycoprotein ligand-1, which is involved in leukocyte-mediated inflammation
-
-
?
3'-phosphoadenylyl sulfate + phytosulfokine-alpha tyrosine
adenosine 3',5'-bisphosphate + phytosulfokine-alpha tyrosine-O-sulfate
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
3'-phosphoadenylyl sulfate + PSGL-1 protein
adenosine 3',5'-bisphosphate + PSGL-1 protein tyrosine-O-sulfate
-
i.e. ATEYEYLDYDFL
-
-
?
3'-phosphoadenylyl sulfate + RNase 9 protein tyrosine
adenosine 3',5'-bisphosphate + RNase 9 protein tyrosine O-sulfate
-
substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview
-
-
?
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine
adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
3'-phosphoadenylyl sulfate + root meristem growth factor 7 tyrosine
adenosine 3',5'-bisphosphate + root meristem growth factor 7 tyrosine-O-sulfate
3'-phosphoadenylyl sulfate + S1P1R tyrosine
adenosine 3',5'-bisphosphate + S1P1R tyrosine O-sulfate
-
type 1 Sphingosine 1-phosphate receptor, S1P1R. Sulfation sequence motif is D-Y19-V/G/-N-Y22-D
-
-
?
3'-phosphoadenylyl sulfate + statherin
adenosine 3',5'-bisphosphate + statherin O-sulfate
-
TPST is responsible for the sulfation of a variety of secretory and membrane proteins, e.g. of the salivary secretory protein, statherin
-
-
?
3'-phosphoadenylyl sulfate + trypsinogen tyrosine
adenosine 3',5'-bisphosphate + trypsinogen tyrosine O-sulfate
sulfation of Tyr154 with Asp as important determinant. Mutation D153N, a naturally occuring polymorphisms in African population, results in a complete loss of trypsinogen sulfation, while mutation of Gly151 and Pro155 of the trypsinogen has no effect on enzyme activity. Genotyping of different populations for the substrate polymorphism, overview
-
-
?
3'-phosphoadenylyl sulfate + TSH receptor
adenosine 3',5'-bisphosphate + TSH receptor O-sulfate
-
tyrosine sulfonation of TSHR by TPST2 is crucial for thyrotropin signaling, TPST1 and TPST2 mutant H266Q are incapable of mediating the signal transduction, overview
-
-
?
3'-phosphoadenylyl sulfate + TSHR tyrosine
adenosine 3',5'-bisphosphate + TSHR tyrosine O-sulfate
-
thyroid-stimulating hormone receptor, TSHR. Sulfation sequence motif is Y385-D/E-Y
-
-
?
3'-phosphoadenylyl sulfate + VTDsYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
-
-
-
-
ir
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYYPDI
-
-
-
-
ir
3'-phosphoadenylyl-sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
TPST-A is involved in the cuticle formation in the nematode. TPST-A RNAi significantly decreases the dityrosine level in the worms, raising the possibility that the sulfation process may be a pre-requisite for the collagen tyrosine cross-linking
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
additional information
?
-
3'-phosphoadenylyl sulfate + phytosulfokine-alpha tyrosine
adenosine 3',5'-bisphosphate + phytosulfokine-alpha tyrosine-O-sulfate
PSK-alpha
-
-
?
3'-phosphoadenylyl sulfate + phytosulfokine-alpha tyrosine
adenosine 3',5'-bisphosphate + phytosulfokine-alpha tyrosine-O-sulfate
PSK-alpha
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
sulfation of protein in the retina, identification of protein substrates, sulfation pattern, overview
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
sulfation of protein in the retina, identification of protein substrates, sulfation pattern, overview
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
TPST is responsible for the sulfation of a variety of secretory and membrane proteins
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
tyrosine O-sulfation is a key post-translational modification that regulates protein-protein interactions in extracellular space
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
tyrosine sulfonation of TSHR by TPST is crucial for thyrotropin signaling, overview
-
-
?
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine
adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro
-
-
?
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine
adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro
-
-
?
3'-phosphoadenylyl sulfate + root meristem growth factor 7 tyrosine
adenosine 3',5'-bisphosphate + root meristem growth factor 7 tyrosine-O-sulfate
RGF7
-
-
?
3'-phosphoadenylyl sulfate + root meristem growth factor 7 tyrosine
adenosine 3',5'-bisphosphate + root meristem growth factor 7 tyrosine-O-sulfate
RGF7
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
enzyme of trans-Golgi network catalyzing post-translational sulfation of a variety of secretory and membrane proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
-
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
involved in post-translational processing of specific PC12 cell proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
post-translational modification of biologically active peptides and proteins
-
-
?
additional information
?
-
-
sulfation patterns, overview
-
-
?
additional information
?
-
-
missense mutation of Tpst2 leads to the autosomal recessive, fetal-onset, severe thyroid hypoplasia phenotype related to TSH hyporesponsiveness in growth-retarded mice, overview
-
-
?
additional information
?
-
-
protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST1, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview
-
-
?
additional information
?
-
-
protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST2, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview
-
-
?
additional information
?
-
-
TPST-2 catalyzes post-translational protein tyrosine O-sulfation, TPST-2, but not TPST-1, deficiency causes male infertility
-
-
?
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Lee, R.W.H.; Huttner, W.B.
Tyrosine-O-sulfated proteins of PC12 pheochromocytoma cells and their sulfation by a tyrosylprotein sulfotransferase
J. Biol. Chem.
258
11326-11334
1983
Rattus norvegicus
brenda
Vargas, F.; Schwartz, J.C.
Apparent identity of cerebral tyrosylsulfotransferase activities using either a cholecystokinin derivative or an acidic amino acid polymer as substrate
FEBS Lett.
211
234-238
1987
Rattus norvegicus
brenda
Rens-Domiano, S.; Roth, J.A.
Characterization of tyrosylprotein sulfotransferase from rat liver and other tissues
J. Biol. Chem.
264
899-905
1989
Oryctolagus cuniculus, Homo sapiens, Mesocricetus auratus, Mus musculus, Rattus norvegicus
brenda
Lin, W.; Larsen, K.; Hortin, G.L.; Roth, J.A.
Recognition of substrates by tyrosylprotein sulfotransferase. Determination of affinity by acidic amino acids near the target sites
J. Biol. Chem.
267
2876-2879
1992
Rattus norvegicus
brenda
Kasinathan, C.; Sundaram, P.; Slomiany, B.L.; Slomiany, A.
Inhibition of tyrosylprotein sulfotransferase by sphingosine and its reversal by acidic phospholipids
Biochemistry
32
1194-1198
1993
Rattus norvegicus
brenda
Niehrs, C.; Huttner, W.B.
Purification and characterization of tyrosylprotein sulfotransferase
EMBO J.
9
35-42
1990
Bos taurus
brenda
Niehrs, C.; Huttner, W.B.; Carvallo, D.; Degryse, E.
Conversion of recombinant hirudin to the natural form by in vitro tyrosine sulfation. Differential substrate specificities of leech and bovine tyrosylprotein sulfotransferases
J. Biol. Chem.
265
9314-9318
1990
Bos taurus, Hirudo medicinalis
brenda
Niehrs, C.; Kraft, M.; Lee, R.W.H.; Huttner, W.B.
Analysis of the substrate specificity of tyrosylprotein sulfotransferase using synthetic peptides
J. Biol. Chem.
265
8525-8532
1990
Bos taurus
brenda
Suiko, M.; Fernando, P.H.P.; Sakakibara, Y.; Nakajima, H.; Liu, M.C.; Abe, S.; Nakatsu, S.
Post-translational modification of protein by tyrosine sulfation: active sulfate PAPS is the essential substrate for this modification
Nucleic Acids Symp. Ser.
27
183-184
1992
Bos taurus
brenda
Kasinathan, C.; Sundaram, P.; Slomiany, B.L.; Slomiany, A.
Identification of tyrosylprotein sulfotransferase in rat gastric mucosa
Enzyme
46
179-187
1993
Rattus norvegicus
brenda
Niehrs, C.; Stinchcombe, J.C.; Huttner, W.B.
Two membrane-bound forms of tyrosylprotein sulfotransferase as revealed by phase partitioning in Triton X-114
Eur. J. Cell Biol.
58
35-43
1992
Bos taurus, Rattus norvegicus
brenda
Sundaram, P.; Slomiany, A.; Slomiany, B.L.; Kasinathan, C.
Tyrosylprotein sulfotransferase in rat submandibular salivary glands
Int. J. Biochem.
24
663-667
1992
Rattus norvegicus
brenda
Lin, W.H.; Roth, J.A.
Characterization of a tyrosylprotein sulfotransferase in human liver
Biochem. Pharmacol.
40
629-635
1990
Homo sapiens
brenda
Rens-Domiano, S.; Hortin, G.L.; Roth, J.A.
Sulfation of tert-butoxycarbonylcholecystokinin and other peptides by rat liver tyrosylprotein sulfotransferase
Mol. Pharmacol.
36
647-653
1989
Rattus norvegicus
brenda
Goettsch, S.; Goettsch, W.; Morawietz, H.; Bayer, P.
Shear stress mediates tyrosylprotein sulfotransferase isoform shift in human endothelial cells
Biochem. Biophys. Res. Commun.
294
541-546
2002
Homo sapiens
brenda
Hanai, H.; Nakayama, D.; Yang, H.; Matsubayashi, Y.; Hirota, Y.; Sakagami, Y.
Existence of a plant tyrosylprotein sulfotransferase: novel plant enzyme catalyzing tyrosine O-sulfation of preprophytosulfokine variants in vitro
FEBS Lett.
470
97-101
2000
Asparagus officinalis, Daucus carota, Solanum lycopersicum, Nicotiana tabacum, Oryza sativa
brenda
Kasinathan, C.; Ramaprasad, P.; William, S.; Espina, N.
Stimulation of tyrosylprotein sulfotransferase activity by ethanol: role of increased enzyme level
Alcohol
15
271-276
1998
Rattus norvegicus
brenda
Kasinathan, C.; Rizwan, M.; Slomiany, A.; Slomiany, B.L.
Effect of sofalcone on tyrosylprotein sulfotransferase
Gen. Pharmacol.
25
1017-1020
1994
Rattus norvegicus
brenda
Kasinathan, C.; Sundaram, R.; William, S.
Effect of prostaglandins on tyrosylprotein sulfotransferase activity in rat submandibular salivary glands
Gen. Pharmacol.
26
577-580
1995
Rattus norvegicus
brenda
Lin, W.H.; Marcucci, K.; Roth, J.A.
Effect of manganese on tyrosylprotein sulfotransferase activity in PC12 cells
Biochem. Pharmacol.
47
1575-1580
1994
Rattus norvegicus
brenda
Moore, K.L.
The biology and enzymology of protein tyrosine O-sulfation
J. Biol. Chem.
278
24243-24246
2003
Homo sapiens, Mammalia, no activity in yeast, no activity in prokaryotes, Drosophila melanogaster (Q9VYB7)
brenda
Ouyang, Y.; Lane, W.S.; Moore, K.L.
Tyrosylprotein sulfotransferase: purification and molecular cloning of an enzyme that catalyzes tyrosine O-sulfation, a common posttranslational modification of eukaryotic proteins
Proc. Natl. Acad. Sci. USA
95
2896-2901
1998
Homo sapiens (O60507), Homo sapiens, Mus musculus (O70281), Mus musculus, Rattus norvegicus
brenda
Ramaprasad, P.; Kasinathan, C.
In vivo induction of tyrosylprotein sulfotransferase by ethanol: role of increased enzyme synthesis
Alcohol. Clin. Exp. Res.
22
1120-1124
1998
Rattus norvegicus
brenda
Ramaprasad, P.; Kasinathan, C.
Isolation of tyrosylprotein sulfotransferase from rat liver
Gen. Pharmacol.
30
555-559
1998
Rattus norvegicus
brenda
Seibert, C.; Cadene, M.; Sanfiz, A.; Chait, B.T.; Sakmar, T.P.
Tyrosine sulfation of CCR5 N-terminal peptide by tyrosylprotein sulfotransferases 1 and 2 follows a discrete pattern and temporal sequence
Proc. Natl. Acad. Sci. USA
99
11031-11036
2002
Homo sapiens
brenda
William, S.; Ramaprasad, P.; Kasinathan, C.
Purification of tyrosylprotein sulfotransferase from rat submandibular salivary glands
Arch. Biochem. Biophys.
338
90-96
1997
Rattus norvegicus
brenda
Mishiro, E.; Liu, M.Y.; Sakakibara, Y.; Suiko, M.; Liu, M.C.
Zebrafish tyrosylprotein sulfotransferase: molecular cloning, expression, and functional characterization
Biochem. Cell Biol.
82
295-303
2004
Danio rerio (Q6WZB4), Danio rerio
brenda
Kim, T.H.; Hwang, S.B.; Jeong, P.Y.; Lee, J.; Cho, J.W.
Requirement of tyrosylprotein sulfotransferase-A for proper cuticle formation in the nematode C. elegans
FEBS Lett.
579
53-58
2005
Caenorhabditis elegans
brenda
Kasinathan, C.; Ramaprasad, P.; Sundaram, P.
Identification and characterization of tyrosylprotein sulfotransferase from human saliva
Int. J. Biol. Sci.
1
141-145
2005
Homo sapiens
brenda
Mishiro, E.; Sakakibara, Y.; Liu, M.; Suiko, M.
Differential enzymatic characteristics and tissue-specific expression of human TPST-1 and TPST-2
J. Biochem.
140
731-737
2006
Homo sapiens (O60507), Homo sapiens (O60704), Homo sapiens
brenda
Hoffhines, A.J.; Damoc, E.; Bridges, K.G.; Leary, J.A.; Moore, K.L.
Detection and purification of tyrosine-sulfated proteins using a novel anti-sulfotyrosine monoclonal antibody
J. Biol. Chem.
281
37877-37887
2006
Mus musculus
brenda
Borghei, A.; Ouyang, Y.B.; Westmuckett, A.D.; Marcello, M.R.; Landel, C.P.; Evans, J.P.; Moore, K.L.
Targeted disruption of tyrosylprotein sulfotransferase-2, an enzyme that catalyzes post-translational protein tyrosine O-sulfation, causes male infertility
J. Biol. Chem.
281
9423-9431
2006
Mus musculus
brenda
Goettsch, S.; Badea, R.A.; Mueller, J.W.; Wotzlaw, C.; Schoelermann, B.; Schulz, L.; Rabiller, M.; Bayer, P.; Hartmann-Fatu, C.
Human TPST1 transmembrane domain triggers enzyme dimerisation and localisation to the Golgi compartment
J. Mol. Biol.
361
436-449
2006
Homo sapiens
brenda
Sasaki, N.; Hosoda, Y.; Nagata, A.; Ding, M.; Cheng, J.M.; Miyamoto, T.; Okano, S.; Asano, A.; Miyoshi, I.; Agui, T.
A mutation in Tpst2 encoding tyrosylprotein sulfotransferase causes dwarfism associated with hypothyroidism
Mol. Endocrinol.
21
1713-1721
2007
Mus musculus
brenda
Kasinathan, C.; Jean, S.; Manowitz, P.
Tyrosine sulfation of arylsulfatase A and its peptide
Protein Pept. Lett.
13
357-361
2006
Homo sapiens
brenda
Seibert, C.; Veldkamp, C.T.; Peterson, F.C.; Chait, B.T.; Volkman, B.F.; Sakmar, T.P.
Sequential tyrosine sulfation of CXCR4 by tyrosylprotein sulfotransferases
Biochemistry
47
11251-11262
2008
Homo sapiens (O60507), Homo sapiens (O60704), Homo sapiens
brenda
Nishimura, M.; Naito, S.
Tissue-specific mRNA expression profiles of human carbohydrate sulfotransferase and tyrosylprotein sulfotransferase
Biol. Pharm. Bull.
30
821-825
2007
Homo sapiens, Homo sapiens (O60507)
brenda
Kasinathan, C.; Gandhi, N.; Ramaprasad, P.; Sundaram, P.; Ramasubbu, N.
Tyrosine sulfation of statherin
Int. J. Biol. Sci.
3
237-241
2007
Homo sapiens
brenda
Danan, L.M.; Yu, Z.; Hoffhines, A.J.; Moore, K.L.; Leary, J.A.
Mass spectrometric kinetic analysis of human tyrosylprotein sulfotransferase-1 and -2
J. Am. Soc. Mass Spectrom.
19
1459-1466
2008
Homo sapiens (O60507), Homo sapiens (O60704), Homo sapiens
brenda
Yu, Y.; Hoffhines, A.J.; Moore, K.L.; Leary, J.A.
Determination of the sites of tyrosine O-sulfation in peptides and proteins
Nat. Methods
4
583-588
2007
Homo sapiens (O60507), Homo sapiens (O60704)
brenda
Ronai, Z.; Witt, H.; Rickards, O.; Destro-Bisol, G.; Bradbury, A.R.; Sahin-Toth, M.
A common African polymorphism abolishes tyrosine sulfation of human anionic trypsinogen (PRSS2)
Biochem. J.
418
155-161
2009
Homo sapiens (O60704), Homo sapiens
brenda
Jen, C.H.; Moore, K.L.; Leary, J.A.
Pattern and temporal sequence of sulfation of CCR5 N-terminal peptides by tyrosylprotein sulfotransferase-2: an assessment of the effects of N-terminal residues
Biochemistry
48
5332-5338
2009
Homo sapiens
brenda
Mhidia, R.; Vallin, A.; Ollivier, N.; Blanpain, A.; Shi, G.; Christiano, R.; Johannes, L.; Melnyk, O.
Synthesis of peptide-protein conjugates using N-succinimidyl carbamate chemistry
Bioconjug. Chem.
21
219-228
2010
Homo sapiens
brenda
Kanan, Y.; Hoffhines, A.; Rauhauser, A.; Murray, A.; Al-Ubaidi, M.R.
Protein tyrosine-O-sulfation in the retina
Exp. Eye Res.
89
559-567
2009
Homo sapiens, Mus musculus
brenda
Westmuckett, A.D.; Hoffhines, A.J.; Borghei, A.; Moore, K.L.
Early postnatal pulmonary failure and primary hypothyroidism in mice with combined TPST-1 and TPST-2 deficiency
Gen. Comp. Endocrinol.
156
145-153
2008
Mus musculus
brenda
Hoffhines, A.J.; Jen, C.H.; Leary, J.A.; Moore, K.L.
Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo
J. Biol. Chem.
284
3096-3105
2009
Mus musculus
brenda
Hosoda, Y.; Sasaki, N.; Agui, T.
Female infertility in grt mice is caused by thyroid hormone deficiency, not by insufficient TPST2 activity in the reproductive organs
J. Vet. Med. Sci.
70
1043-1049
2008
Mus musculus
brenda
Stone, M.J.; Chuang, S.; Hou, X.; Shoham, M.; Zhu, J.Z.
Tyrosine sulfation: an increasingly recognised post-translational modification of secreted proteins
N. Biotechnol.
25
299-317
2009
Bos taurus, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus
brenda
Chen, B.H.; Wang, C.C.; Lu, L.Y.; Hung, K.S.; Yang, Y.S.
Fluorescence assay for protein post-translational tyrosine sulfation
Anal. Bioanal. Chem.
405
1425-1429
2013
Drosophila melanogaster
brenda
Lu, L.; Chen, B.; Wu, J.; Wang, C.; Chen, D.; Yang, Y.
Implantation of post-translational tyrosylprotein sulfation into a prokaryotic expression system
ChemBioChem
12
377-379
2011
Homo sapiens
brenda
Danan, L.M.; Yu, Z.; Ludden, P.J.; Jia, W.; Moore, K.L.; Leary, J.A.
Catalytic mechanism of Golgi-resident human tyrosylprotein sulfotransferase-2: a mass spectrometry approach
J. Am. Soc. Mass Spectrom.
21
1633-1642
2010
Homo sapiens
brenda
Kim, T.H.; Kim, D.H.; Nam, H.W.; Park, S.Y.; Shim, J.; Cho, J.W.
Tyrosylprotein sulfotransferase regulates collagen secretion in Caenorhabditis elegans
Mol. Cells
29
413-418
2010
Caenorhabditis elegans, Caenorhabditis elegans N2
brenda
Teramoto, T.; Fujikawa, Y.; Kawaguchi, Y.; Kurogi, K.; Soejima, M.; Adachi, R.; Nakanishi, Y.; Mishiro-Sato, E.; Liu, M.C.; Sakakibara, Y.; Suiko, M.; Kimura, M.; Kakuta, Y.
Crystal structure of human tyrosylprotein sulfotransferase-2 reveals the mechanism of protein tyrosine sulfation reaction
Nat. Commun.
4
1572
2013
Homo sapiens (O60704), Homo sapiens
brenda
Zhou, W.; Wei, L.; Xu, J.; Zhai, Q.; Jiang, H.; Chen, R.; Chen, Q.; Sun, J.; Chu, J.; Zhu, L.; Liu, C.M.; Li, C.
Arabidopsis Tyrosylprotein sulfotransferase acts in the auxin/PLETHORA pathway in regulating postembryonic maintenance of the root stem cell niche
Plant Cell
22
3692-3709
2010
Arabidopsis thaliana
brenda
Zhou, W.; Duckworth, B.P.; Geraghty, R.J.
Fluorescent peptide sensors for tyrosylprotein sulfotransferase activity
Anal. Biochem.
461
1-6
2014
Homo sapiens (O60507), Homo sapiens (O60704), Homo sapiens
brenda
Marforio, T.D.; Giacinto, P.; Bottoni, A.; Calvaresi, M.
Computational evidence for the catalytic mechanism of tyrosylprotein sulfotransferases: a density functional theory investigation
Biochemistry
54
4404-4410
2015
Homo sapiens (O60704), Homo sapiens
brenda
Nedumpully-Govindan, P.; Li, L.; Alexov, E.G.; Blenner, M.A.; Ding, F.
Structural and energetic determinants of tyrosylprotein sulfotransferase sulfation specificity
Bioinformatics
30
2302-2309
2014
Homo sapiens (O60507), Homo sapiens (O60704)
brenda
Hartmann-Fatu, C.; Bayer, P.
Determinants of tyrosylprotein sulfation coding and substrate specificity of tyrosylprotein sulfotransferases in metazoans
Chem. Biol. Interact.
259
17-22
2016
Homo sapiens (O60507), Homo sapiens (O60704)
brenda
Hartmann-Fatu, C.; Trusch, F.; Moll, C.N.; Michin, I.; Hassinen, A.; Kellokumpu, S.; Bayer, P.
Heterodimers of tyrosylprotein sulfotransferases suggest existence of a higher organization level of transferases in the membrane of the trans-Golgi apparatus
J. Mol. Biol.
427
1404-1412
2015
Homo sapiens
brenda
Jiang, Z.; Zhu, J.; Ma, Y.; Hong, C.; Xiao, S.; Jin, L.
Tyrosylprotein sulfotransferase 1 expression is negatively correlated with c-Met and lymph node metastasis in human lung cancer
Mol. Med. Rep.
12
5217-5222
2015
Homo sapiens (O60507), Homo sapiens
brenda
Singh, W.; Karabencheva-Christova, T.; Black, G.; Sparagano, O.; Christov, C.
Conformational flexibility influences structure-function relationships in tyrosyl protein sulfotransferase-2
RSC Adv.
6
11344-11352
2016
Homo sapiens (O60704)
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brenda
Singh, W.; Karabencheva-Christova, T.; Sparagano, O.; Black, G.; Petrov, P.; Christov, C.
Dimerization and ligand binding in tyrosylprotein sulfotransferase-2 are influenced by molecular motions
RSC Adv.
6
18542-18548
2016
Homo sapiens (O60704)
-
brenda
Wang, C.C.; Chen, B.H.; Lu, L.Y.; Hung, K.S.; Yang, Y.S.
Preparation of tyrosylprotein sulfotransferases for in vitro one-pot enzymatic synthesis of sulfated proteins/peptides
ACS Omega
3
11633-11642
2018
Homo sapiens (O60507), Homo sapiens (O60704), Homo sapiens, Drosophila melanogaster (Q9VYB7), Drosophila melanogaster
brenda
Huang, B.Y.; Chen, P.C.; Chen, B.H.; Wang, C.C.; Liu, H.F.; Chen, Y.Z.; Chen, C.S.; Yang, Y.S.
High-throughput screening of sulfated proteins by using a genome-wide proteome microarray and protein tyrosine sulfation system
Anal. Chem.
89
3278-3284
2017
Drosophila melanogaster (Q9VYB7)
brenda
Zhou, W.; Wang, Y.; Xie, J.; Geraghty, R.J.
A fluorescence-based high-throughput assay to identify inhibitors of tyrosylprotein sulfotransferase activity
Biochem. Biophys. Res. Commun.
482
1207-1212
2017
Homo sapiens (O60507), Homo sapiens (O60704)
brenda
Byrne, D.P.; Li, Y.; Ngamlert, P.; Ramakrishnan, K.; Eyers, C.E.; Wells, C.; Drewry, D.H.; Zuercher, W.J.; Berry, N.G.; Fernig, D.G.; Eyers, P.A.
New tools for evaluating protein tyrosine sulfation tyrosylprotein sulfotransferases (TPSTs) are novel targets for RAF protein kinase inhibitors
Biochem. J.
475
2435-2455
2018
Homo sapiens (O60507), Homo sapiens (O60704)
brenda
Yeoh, S.; Bayliss, R.
New tools for evaluating protein tyrosine sulfation and carbohydrate sulfation
Biochem. J.
475
3035-3037
2018
Homo sapiens (O60507)
brenda
Seibert, C.; Sanfiz, A.; Sakmar, T.P.; Veldkamp, C.T.
Preparation and analysis of N-terminal chemokine receptor sulfopeptides using tyrosylprotein sulfotransferase enzymes
Methods Enzymol.
570
357-388
2016
Homo sapiens (O60507), Homo sapiens (O60704)
brenda
Zhong, Y.; Xie, J.; Wen, S.; Wu, W.; Tan, L.; Lei, M.; Shi, H.; Zhu, J.K.
TPST is involved in fructose regulation of primary root growth in Arabidopsis thaliana
Plant Mol. Biol.
103
511-525
2020
Arabidopsis thaliana (Q3EDG5), Arabidopsis thaliana Col-0 (Q3EDG5)
brenda
Tanaka, S.; Nishiyori, T.; Kojo, H.; Otsubo, R.; Tsuruta, M.; Kurogi, K.; Liu, M.C.; Suiko, M.; Sakakibara, Y.; Kakuta, Y.
Structural basis for the broad substrate specificity of the human tyrosylprotein sulfotransferase-1
Sci. Rep.
7
8776
2017
Homo sapiens (O60507), Homo sapiens
brenda