General Stability | Organism |
---|---|
incubation with 30% glycerol at 4°C for 3 h stabilizes activity | Arthrobacter allantoicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycolate | 18% inhibition at 17 mM | Arthrobacter allantoicus | |
glyoxylate | non-competitive inhibition, Ki: 83 mM | Arthrobacter allantoicus | |
L-lactate | 10% inhibition at 17 mM | Arthrobacter allantoicus | |
Zn2+ | 40% inhibition at 0.5 mM | Arthrobacter allantoicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NADP+ | - |
Arthrobacter allantoicus | |
2.16 | - |
NAD+ | - |
Arthrobacter allantoicus | |
9.1 | - |
(R,S)-ureidoglycolate | - |
Arthrobacter allantoicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-ureidoglycolate + NAD(P)+ | Arthrobacter allantoicus | strong substrate specificity, involved in the degradation of allantoin to glyoxylate in microbes and lower animals | oxalureate + NAD(P)H | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter allantoicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Arthrobacter allantoicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Arthrobacter allantoicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.6 | - |
- |
Arthrobacter allantoicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-ureidoglycolate + NAD+ | - |
Arthrobacter allantoicus | oxalureate + NADH + H+ | - |
r | |
(S)-ureidoglycolate + NAD(P)+ | strong substrate specificity, involved in the degradation of allantoin to glyoxylate in microbes and lower animals | Arthrobacter allantoicus | oxalureate + NAD(P)H | - |
ir |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
unstable above | Arthrobacter allantoicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.4 | sensitive to ionic strength | Arthrobacter allantoicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
decrease of activity below | Arthrobacter allantoicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arthrobacter allantoicus | |
NADP+ | - |
Arthrobacter allantoicus |