Cloned (Comment) | Organism |
---|---|
gene TK1968, recombinant coexpression of wild-type and mutant Tk-KPR enzyme dimers carrying a His6-tag and a Strep-tag on each monomer in Escherichia coli | Thermococcus kodakarensis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant tagged enzyme mutant C84A, hanging drop vapour diffusion method, mixing of 0.001 ml of 10 mg /ml protein in 10 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, mM NADH, and 1 mM 2-oxopantoate, with 0.001 ml of reservoir solution containing 100 mM sodium acetate, pH 5.5, 10% w/v PEG 3350, 20% v/v 2-propanol, and equilibration against 0.5 ml reservoir solution, at 20°C, 1 week, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement method for modeling | Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
C84A | site-directed mutagenesis | Thermococcus kodakarensis |
W129A | site-directed mutagenesis | Thermococcus kodakarensis |
Y60A | site-directed mutagenesis | Thermococcus kodakarensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | feedback inhibition | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-pantoate + NADP+ | Thermococcus kodakarensis | - |
2-dehydropantoate + NADPH + H+ | - |
r | |
(R)-pantoate + NADP+ | Thermococcus kodakarensis ATCC BAA-918 | - |
2-dehydropantoate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JGC2 | - |
- |
Thermococcus kodakarensis ATCC BAA-918 | Q5JGC2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant Tk-KPR enzyme dimers carrying a His6-tag and a Strep-tag on each monomer from Escherichia coli by affinity chromatography | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-pantoate + NADP+ | - |
Thermococcus kodakarensis | 2-dehydropantoate + NADPH + H+ | - |
r | |
(R)-pantoate + NADP+ | - |
Thermococcus kodakarensis ATCC BAA-918 | 2-dehydropantoate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | crystal structure analysis | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
ketopantoate reductase | - |
Thermococcus kodakarensis |
KPR | - |
Thermococcus kodakarensis |
Tk-KPR | - |
Thermococcus kodakarensis |
TK1968 | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
assay at | Thermococcus kodakarensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermococcus kodakarensis |
General Information | Comment | Organism |
---|---|---|
physiological function | ketopantoate reductase catalyzes the NAD(P)H-dependent reduction of 2-oxopantoate which is a step in the biosynthesis of coenzyme A | Thermococcus kodakarensis |