Cloned (Comment) | Organism |
---|---|
functional recombinant expression in Escherichia coli | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
L181F | random mutagenesis, A site mutant | Priestia megaterium |
L181F/I263N | random mutagenesis, A site mutant | Priestia megaterium |
additional information | whole-cell-catalyzed multiple regio- and stereoselective functionalizations in cascade reactions enabled by directed evolution: in Escherichia coli, expression of the recombinant enzyme leads to four different mutant whole cells, each leading to one of the three possible stereoisomeric forms of 1,2-cyclohexanediol, namely (R,R)-5, (S,S)-5, (S,R)-5 (meso), or (R,S)-5 (also meso), in a multi-step sequence, overview. Starting from the achiral substrates cyclohexane, cyclohexanol, or cyclohexanone, cyclohexane-1,2-diols as functionalized products are shown to be accessible in three different stereoisomeric forms | Priestia megaterium |
V78I/A82F/A328F/T438I | random mutagenesis, B site mutant | Priestia megaterium |
V78I/A82I/A328N | random mutagenesis, B site mutant | Priestia megaterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-cyclohexane-1,2-diol + NAD+ | Priestia megaterium | - |
2-hydroxycyclohexan-1-one + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-cyclohexane-1,2-diol + NAD+ | - |
Priestia megaterium | 2-hydroxycyclohexan-1-one + NADH + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Priestia megaterium |