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Literature summary for 1.1.1.175 extracted from

  • Toivari, M.; Nygard, Y.; Kumpula, E.P.; Vehkomaeki, M.L.; Bencina, M.; Valkonen, M.; Maaheimo, H.; Andberg, M.; Koivula, A.; Ruohonen, L.; Penttilae, M.; Wiebe, M.G.
    Metabolic engineering of Saccharomyces cerevisiae for bioconversion of D-xylose to D-xylonate (2012), Metab. Eng., 14, 427-436.
    View publication on PubMed

Application

Application Comment Organism
synthesis expression of gene xylB in Saccharomyces cerevisiae results in production of 17 g D-xylonate/l at 0.23g/l/h from 23 g D-xylose/l. D-Xylonate accumulates intracellularly to 70 mg/g, xylitol to 18 mg/g. Cells expressing D-xylonolactone lactonase xylC from Caulobacter crescentus with xylB initially produce more extracellular D-xylonate than cells lacking xylC at both pH5.5 and pH3, and sustain higher production at pH3. Cell vitality and viability decreases during D-xylonate production at pH 3.0 Caulobacter vibrioides

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Caulobacter vibrioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
D-xylose 25┬░C, pH 9.0 Caulobacter vibrioides
0.24
-
D-xylose 25┬░C, pH 7.0 Caulobacter vibrioides
0.26
-
NAD+ 25┬░C, pH 6.8 Caulobacter vibrioides
0.31
-
NAD+ 25┬░C, pH 7.0 Caulobacter vibrioides
0.4
-
D-xylose 25┬░C, pH 6.8 Caulobacter vibrioides
0.46
-
NAD+ 25┬░C, pH 9.0 Caulobacter vibrioides
40
-
L-arabinose 25┬░C, pH 9.0 Caulobacter vibrioides
100
-
L-arabinose 25┬░C, pH 7.0 Caulobacter vibrioides
180
-
L-arabinose 25┬░C, pH 6.8 Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NAD+ Caulobacter vibrioides
-
D-xylonolactone + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides B8H1Z0 putative
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NAD+
-
Caulobacter vibrioides D-xylonolactone + NADH + H+
-
?
L-arabinose + NAD+
-
Caulobacter vibrioides L-arabinono-1,4-lactone + NADH + H+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22.3
-
L-arabinose 25┬░C, pH 6.8 Caulobacter vibrioides
22.7
-
D-xylose 25┬░C, pH 9.0 Caulobacter vibrioides
24.2
-
L-arabinose 25┬░C, pH 9.0 Caulobacter vibrioides
25.8
-
NAD+ 25┬░C, pH 6.8 Caulobacter vibrioides
26.2
-
D-xylose 25┬░C, pH 6.8 Caulobacter vibrioides
29.2
-
L-arabinose 25┬░C, pH 7.0 Caulobacter vibrioides
30.3
-
D-xylose 25┬░C, pH 7.0 Caulobacter vibrioides
31
-
NAD+ 25┬░C, pH 9.0 Caulobacter vibrioides
35.7
-
NAD+ 25┬░C, pH 7.0 Caulobacter vibrioides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Caulobacter vibrioides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.12
-
L-arabinose 25┬░C, pH 6.8 Caulobacter vibrioides
0.28
-
L-arabinose 25┬░C, pH 7.0 Caulobacter vibrioides
0.6
-
L-arabinose 25┬░C, pH 9.0 Caulobacter vibrioides
66.7
-
D-xylose 25┬░C, pH 6.8 Caulobacter vibrioides
66.7
-
NAD+ 25┬░C, pH 9.0 Caulobacter vibrioides
101.7
-
NAD+ 25┬░C, pH 6.8 Caulobacter vibrioides
115
-
NAD+ 25┬░C, pH 7.0 Caulobacter vibrioides
125
-
D-xylose 25┬░C, pH 7.0 Caulobacter vibrioides
280
-
D-xylose 25┬░C, pH 9.0 Caulobacter vibrioides