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Literature summary for 1.1.1.183 extracted from

  • Hassan, M.; Maarof, N.D.; Ali, Z.M.; Noor, N.M.; Othman, R.; Mori, N.
    Monoterpene alcohol metabolism: identification, purification, and characterization of two geraniol dehydrogenase isoenzymes from Polygonum minus leaves (2012), Biosci. Biotechnol. Biochem., 76, 1463-1470.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information adding sodium metabisulfite, ascorbic acid, dithiothreitol, and EDTA to the extraction buffer and using Tris-HCl (pH 7.5) or potassium phosphate (pH 7.5) fails to enhance enzyme activity Persicaria minor

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl isoform geraniol-DH I shows 72% residual activity and geraniol-DH II shows 75% residual activity at 1 mM Persicaria minor
cyanide isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM Persicaria minor
iodoacetamide isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM Persicaria minor
MgCl2 isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 45% residual activity at 5 mM Persicaria minor
Sodium azide isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 90% residual activity at 1 mM Persicaria minor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
NADP+ isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
0.0588
-
NADP+ isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
0.185
-
geraniol isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
0.4
-
geraniol isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
0.435
-
cinnamyl alcohol isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
0.556
-
cinnamyl alcohol isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
0.714
-
nerol isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
0.741
-
linalool isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
1.212
-
nerol isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
1.333
-
linalool isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
2.222
-
3,7-dimethyloct-6-en-1-ol isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
10
-
3,7-dimethyloct-6-en-1-ol isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE Persicaria minor
58000
-
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE Persicaria minor
60000
-
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE Persicaria minor
62000
-
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE Persicaria minor
100000
-
isoform geraniol-DH I, gel filtration Persicaria minor
120000
-
isoform geraniol-DH II, gel filtration Persicaria minor

Organism

Organism UniProt Comment Textmining
Persicaria minor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Toyopearl 650M column chromatography, phenyl Toyopearl 650 M column chromatography, and AF-Red Toyopearl 650ML column chromatography Persicaria minor

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Persicaria minor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.002
-
cell free extract, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
0.175
-
isoform geraniol-DH I, after 116.7fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor
0.261
-
isoform geraniol-DH II, after 173.8fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37┬░C Persicaria minor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-citronellol + NADP+ compared to geraniol, isoform geraniol-DH I shows 72% activity and isoform geraniol-DH II shows 63% activity with this substrate Persicaria minor ?
-
?
borneol + NADP+ compared to geraniol, isoform geraniol-DH I shows 52% activity and isoform geraniol-DH II shows 29% activity with this substrate Persicaria minor ?
-
?
carveol + NADP+ compared to geraniol, isoform geraniol-DH I shows 44% activity with this substrate Persicaria minor ?
-
?
cinnamyl alcohol + NADP+ compared to geraniol, isoform geraniol-DH I shows 60% activity and isoform geraniol-DH II shows 50% activity with this substrate Persicaria minor ?
-
?
geraniol + NADP+ 100% activity Persicaria minor geranial + NADPH + H+
-
?
linalool + NADP+ compared to geraniol, isoform geraniol-DH I shows 42% activity and isoform geraniol-DH II shows 92% activity with this substrate Persicaria minor ?
-
?
additional information tetrahydrogeraniol, tetrahydrolinalool, menthol, benzyl alcohol, and farnesol are no substrates for both isoforms. Isoform geraniol-DH II does not react with 3,7-dihydrolinalool, and isoform geraniol-DH I does not react with carveol Persicaria minor ?
-
?
nerol + NADP+ compared to geraniol, isoform geraniol-DH I shows 64% activity and isoform geraniol-DH II shows 50% activity with this substrate Persicaria minor ?
-
?
p-cumic alcohol + NADP+ compared to geraniol, isoform geraniol-DH I shows 56% activity and isoform geraniol-DH II shows 46% activity with this substrate Persicaria minor ?
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE Persicaria minor
heterodimer 1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE Persicaria minor

Synonyms

Synonyms Comment Organism
geraniol-DH I isoform Persicaria minor
geraniol-DH II isoform Persicaria minor
NADP+-dependent geraniol dehydrogenase
-
Persicaria minor

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
40
-
isoforms geraniol-DH I and geraniol-DH II Persicaria minor

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
30 70 rapid inactiviation of isoform geraniol-DH I occurs from 30-60┬░C with 60% activity at 40┬░C and 40% residual activity at 60┬░C. At 70┬░C the isoform shows less than 20% residual activity. Isoform geraniol-DH II retains over 80% activity after 10 min at 40┬░C, while from 30-50┬░C the activity decreases slightly and is lost at temperatures above 50┬░C Persicaria minor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
isoform geraniol-DH I Persicaria minor
9.5
-
isoform geraniol-DH II Persicaria minor

pH Range

pH Minimum pH Maximum Comment Organism
6 10 isoforms geraniol-DH I and geraniol-DH II show less than 40% activity below pH 6.5. Isoform geraniol-DH II shows no activity at pH 10.0, while isoform geraniol-DH I shows about 50% activity. Both isoforms are inactive at pH 6.0 and below Persicaria minor

pI Value

Organism Comment pI Value Maximum pI Value
Persicaria minor isoform geraniol-DH II, isoelectric focusing
-
3.5
Persicaria minor isoform geraniol-DH I, isoelectric focusing
-
8.8