Cloned (Comment) | Organism |
---|---|
into vector pET-28a and expressed in Escherichia coli BL21 (DE3) | Mus musculus |
into vector pET-28a and expressed in Escherichia coli BL21 (DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | heat treatment of AKR1B1 at 100°C for 5 min completely inactivates the prostaglandin F2alpha synthase activity, which is strictly dependent on the presence of NADPH | Homo sapiens | |
additional information | heat treatment of AKR1B3 and AKR1B7 at 100°C for 5 min completely inactivates the prostaglandin F2alpha synthase activities, which is strictly dependent on the presence of NADPH | Mus musculus | |
sorbinil | - |
Homo sapiens | |
sorbinil | significantly inhibits the prostaglandin F2alpha synthase activity of AKR1B3 in a non-competitive fashion, whereas AKR1B7 is not sensitive to the inhibitor | Mus musculus | |
Tolrestat | - |
Homo sapiens | |
Tolrestat | significantly inhibits the prostaglandin F2alpha synthase activity of AKR1B3 in a non-competitive fashion, whereas AKR1B7 is not sensitive to the inhibitor | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
prostaglandin H2 | - |
Homo sapiens | |
0.0038 | - |
prostaglandin H2 | AKR1B7 | Mus musculus | |
0.0057 | - |
NADPH | - |
Homo sapiens | |
0.0074 | - |
NADPH | AKR1B3 | Mus musculus | |
0.0092 | - |
NADPH | AKR1B7 | Mus musculus | |
0.0093 | - |
prostaglandin H2 | AKR1B3 | Mus musculus | |
0.018 | - |
prostaglandin H2 | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Mus musculus |
35000 | - |
x * 35000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P15121 | - |
- |
Homo sapiens | P42330 | - |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to apparent homogeneity, on Ni-NTA resin and by gel filtration | Mus musculus |
to apparent homogeneity, on Ni-NTA resin and by gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | AKR1B3 | Mus musculus | - |
lung | - |
Homo sapiens | - |
placenta | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prostaglandin H2 + NADPH + H+ | aldo-keto reductase has prostaglandin F2alpha synthase activity. AKR1B1 is a more efficient prostaglandin F2alpha synthase than AKR1C3 | Homo sapiens | (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | aldo-keto reductases AKR1B3 and AKR1B7 have prostaglandin F2alpha synthase activity | Mus musculus | (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Mus musculus |
? | x * 35000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
AKR1B1 | - |
Homo sapiens |
AKR1B3 | - |
Mus musculus |
AKR1B7 | - |
Mus musculus |
AKR1C3 | - |
Homo sapiens |
aldo-keto reductase | - |
Mus musculus |
aldo-keto reductase | - |
Homo sapiens |
PGFS | - |
Mus musculus |
PGFS | - |
Homo sapiens |
prostaglandin F2alpha synthase | - |
Mus musculus |
prostaglandin F2alpha synthase | - |
Homo sapiens |
vas deferens protein | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mus musculus | |
NADPH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00026 | - |
Tolrestat | AKR1B3 | Mus musculus | |
0.00089 | - |
sorbinil | AKR1B3 | Mus musculus | |
0.0036 | - |
Tolrestat | - |
Homo sapiens | |
0.0217 | - |
sorbinil | - |
Homo sapiens |