Literature summary for 1.1.1.198 extracted from

Khine, A.; Chen, H.; Huang, K.; Ko, T.
Structural characterization of borneol dehydrogenase from Pseudomonas sp. TCU-HL1 (2020), Acta Crystallogr. Sect. F, 76, 309-313 .

Search for more literature for 1.1.1.198

Application

Application	Comment	Organism
synthesis	recombinant borneol dehydrogenase is found in inclusion bodies when expressed in Escherichia coli. Changing the medium from lysogeny broth to Terrific Broth yield a soluble form of the enzyme	Pseudomonas sp. TCU-HL1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pseudomonas sp. TCU-HL1
expression in Escherichia coli	Pseudomonas sp. TCU-HL1

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 30% (w/v) PEG 8000, 0.1 M sodium acetate pH 4.5, 0.2 M lithium sulfate	Pseudomonas sp. TCU-HL1
to 1.84 A resolution. Protein features a seven-stranded antiparallel beta-sheet flanked by three alpha-helices on each side	Pseudomonas sp. TCU-HL1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(+)-borneol + NAD+	Pseudomonas sp. TCU-HL1	-	(+)-camphor + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. TCU-HL1	A0A1B3EB36	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate saturation, phenyl Sepharose column chromatography, and Q Sepharose column chromatography	Pseudomonas sp. TCU-HL1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-borneol + NAD+	-	Pseudomonas sp. TCU-HL1	(+)-camphor + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
BDH	-	Pseudomonas sp. TCU-HL1
BDH1	-	Pseudomonas sp. TCU-HL1
borneol dehydrogenase	-	Pseudomonas sp. TCU-HL1
THL1_4180	-	Pseudomonas sp. TCU-HL1

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Pseudomonas sp. TCU-HL1

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Release 2023.1 (January 2023)