Cloned (Comment) | Organism |
---|---|
gene bceC, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Burkholderia cepacia |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged BceC, 0.001 ml of protein solution containing 5 mg/ml protein in 25 mM Tris-HCl, pH 8.3, 50 mM NaCl, 2.5 mM DTT, 0.25 mM UDP-GlcA, and 0.5 mM NAD+, is mixed with 0.001 ml of precipitant solution containing 200 mM ammonium sulfate, 100 mM sodium acetate, pH 4.5, 11% w/v PEG 4000, and 50 mM NaF, method optimiization, X-ray diffraction structure determination and analysis at 2.09 A resolution, molecular replacement | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52600 | - |
x * 52600, recombinant His6-tagged BceC, SDS-PAGE | Burkholderia cepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2 NAD+ + H2O | Burkholderia cepacia | - |
UDP-glucuronic acid + 2 NADH + 2 H+ | - |
? | |
UDP-glucose + 2 NAD+ + H2O | Burkholderia cepacia IST408 | - |
UDP-glucuronic acid + 2 NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
gene bceC | - |
Burkholderia cepacia IST408 | - |
gene bceC | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged BceC from Escherichia coli by affinity chromatography | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2 NAD+ + H2O | - |
Burkholderia cepacia | UDP-glucuronic acid + 2 NADH + 2 H+ | - |
? | |
UDP-glucose + 2 NAD+ + H2O | - |
Burkholderia cepacia IST408 | UDP-glucuronic acid + 2 NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52600, recombinant His6-tagged BceC, SDS-PAGE | Burkholderia cepacia |
Synonyms | Comment | Organism |
---|---|---|
BceC | - |
Burkholderia cepacia |
UDP-glucose dehydrogenase | - |
Burkholderia cepacia |
uridine-5'-diphosphoglucose dehydrogenase | - |
Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Burkholderia cepacia |